Prkacb protein kinase, cAMP dependent, catalytic, beta [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkacbprovided by MGI
Official Full Name: protein kinase, cAMP dependent, catalytic, betaprovided by MGI
Primary source: MGI:MGI:97594
See related: Ensembl:ENSMUSG00000005034 AllianceGenome:MGI:97594
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CbPKA; Pkacb; PKA C-beta
Summary: Enables ATP binding activity; cAMP-dependent protein kinase activity; and magnesium ion binding activity. Involved in protein phosphorylation. Acts upstream of or within negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning; neural tube closure; and regulation of protein processing. Located in ciliary base. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system gland; liver and biliary system; and nervous system. Orthologous to human PRKACB (protein kinase cAMP-activated catalytic subunit beta). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cortex adult (RPKM 53.9), CNS E18 (RPKM 48.1) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 H2; 3 72.28 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (146435334..146518691, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (146729579..146812946, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Protein kinase A signaling explains vasopressin-mediated regulation of membrane trafficking and gene transcription.
Title: Systems-level identification of PKA-dependent signaling in epithelial cells.
We suggest a role of the PRKACB gene splice variant Cbeta2 in regulating innate as well as adaptive immune sensitivity in vivo.
Title: Ablation of the Cβ2 subunit of PKA in immune cells leads to increased susceptibility to systemic inflammation in mice.
Gli2 and Gli3 are dephosphorylated and activated in cilia and that impaired Gli2 and Gli3 processing in Ta3 mutant is at least in part due to a decrease in Gli2 and Gli3 phosphorylation.
Title: PKA-mediated Gli2 and Gli3 phosphorylation is inhibited by Hedgehog signaling in cilia and reduced in Talpid3 mutant.
Data indicate that TRPV4 activity and TRPV4 trafficking are under discrete but synergistic control of PKC- and PKA-dependent pathways.
Title: Discrete control of TRPV4 channel function in the distal nephron by protein kinases A and C.
Studies identified three novel alternatively spliced transcript variants of the mouse protein kinase A catalytic beta subunit (Cbeta) gene designated Cbeta5, Cbeta6 and Cbeta7.
Title: Identification and expression analysis of three novel splice variants of protein kinase A catalytic β subunit gene in the mouse using combinatorial in silico and molecular biology approaches.
These data suggest that both developmental and tumor phenotypes caused by Prkar1a mutation result from excess PKA activity due to PKA-Ca.
Title: Differential role of PKA catalytic subunits in mediating phenotypes caused by knockout of the Carney complex gene Prkar1a.
Murine lymphoid tissues express a protein that is a homologue of human and bovine Cbeta2
Title: Identification, cloning and characterization of a novel 47 kDa murine PKA C subunit homologous to human and bovine Cbeta2.
PKCbetaII is a target for colon cancer chemoprevention and the PKCbeta-selective inhibitor enzastaurin may represent an effective chemopreventive agent in patients at high risk for colon cancer.
Title: Protein kinase Cbeta is an effective target for chemoprevention of colon cancer.
crystal structure of the catalytic subunit in complex with ADP, aluminum fluoride, Mg2+ ions and a substrate peptide was determined at 2.0 A resolution
Title: Crystal structure of a transition state mimic of the catalytic subunit of cAMP-dependent protein kinase.
glucose and glucagon-like polypeptide-1 caused translocation of Calpha to the nucleus and of Cbeta to the plasma membrane and the nucleus, but did not affect the distribution of Cgamma in pancreatic beta-cells
Title: Protein kinase A translocation and insulin secretion in pancreatic beta-cells: studies with adenylate cyclase toxin from Bordetella pertussis.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Prkacb (e-PCR), detects polymorphism
- UniSTS:507175

Prkacb (e-PCR), detects polymorphism
- UniSTS:144190

RH133906 (e-PCR)
- UniSTS:217189

X61434 (e-PCR)
- UniSTS:125826

Prkacb (e-PCR), detects polymorphism
- UniSTS:507858

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables cAMP-dependent protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cAMP-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	TAS Traceable Author Statement more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in adenylate cyclase-modulating G protein-coupled receptor signaling pathway	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of TORC1 signaling	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of meiotic cell cycle	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of smoothened signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within neural tube closure	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein kinase A signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of protein processing	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of cAMP-dependent protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cAMP-dependent protein kinase complex	ISO Inferred from Sequence Orthology more info
part_of cAMP-dependent protein kinase complex	TAS Traceable Author Statement more info	PubMed
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in ciliary base	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cAMP-dependent protein kinase catalytic subunit beta

Names: Cbeta2 PKA; cAMP-dependent protein kinase C beta

NP_001157670.1

EC 2.7.11.11

NP_001157671.1

EC 2.7.11.11

NP_001157672.1

EC 2.7.11.11

NP_001397387.1

EC 2.7.11.11

NP_001397388.1

EC 2.7.11.11

NP_001397389.1

EC 2.7.11.11

NP_035230.1

EC 2.7.11.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001164198.2 → NP_001157670.1 cAMP-dependent protein kinase catalytic subunit beta isoform 2

See identical proteins and their annotated locations for NP_001157670.1

Status: VALIDATED

Source sequence(s)

AC114618

Consensus CDS

CCDS51090.1

UniProtKB/Swiss-Prot

P68181

Related

ENSMUSP00000101743.2, ENSMUST00000106137.8

Conserved Domains (1) summary

cd14209
Location:29 → 318

STKc_PKA; Catalytic subunit of the Serine/Threonine Kinase, cAMP-dependent protein kinase
NM_001164199.2 → NP_001157671.1 cAMP-dependent protein kinase catalytic subunit beta isoform 3

See identical proteins and their annotated locations for NP_001157671.1

Status: VALIDATED

Source sequence(s)

AC114618

Consensus CDS

CCDS51091.1

UniProtKB/Swiss-Prot

P68181

Related

ENSMUSP00000101744.2, ENSMUST00000106138.8

Conserved Domains (1) summary

cd14209
Location:30 → 319

STKc_PKA; Catalytic subunit of the Serine/Threonine Kinase, cAMP-dependent protein kinase
NM_001164200.2 → NP_001157672.1 cAMP-dependent protein kinase catalytic subunit beta isoform 4

See identical proteins and their annotated locations for NP_001157672.1

Status: VALIDATED

Source sequence(s)

AC114618

Consensus CDS

CCDS51092.1

UniProtKB/Swiss-Prot

P68181

Related

ENSMUSP00000005164.8, ENSMUST00000005164.12

Conserved Domains (2) summary

PTZ00263
Location:79 → 398

PTZ00263; protein kinase A catalytic subunit; Provisional

cd14209
Location:89 → 378

STKc_PKA; Catalytic subunit of the Serine/Threonine Kinase, cAMP-dependent protein kinase
NM_001410458.1 → NP_001397387.1 cAMP-dependent protein kinase catalytic subunit beta isoform 5

Status: VALIDATED

Source sequence(s)

AC114618
NM_001410459.1 → NP_001397388.1 cAMP-dependent protein kinase catalytic subunit beta isoform 6

Status: VALIDATED

Source sequence(s)

AC114618, AC115889
NM_001410460.1 → NP_001397389.1 cAMP-dependent protein kinase catalytic subunit beta isoform 6

Status: VALIDATED

Source sequence(s)

AC114618, AC115889
NM_011100.5 → NP_035230.1 cAMP-dependent protein kinase catalytic subunit beta isoform 1

See identical proteins and their annotated locations for NP_035230.1

Status: VALIDATED

Source sequence(s)

AC114618, AC115889

Consensus CDS

CCDS17906.1

UniProtKB/Swiss-Prot

P68181, Q3TQH5, Q3UDD0, Q3UTH5

Related

ENSMUSP00000099573.4, ENSMUST00000102515.10

Conserved Domains (1) summary

cd14209
Location:42 → 331

STKc_PKA; Catalytic subunit of the Serine/Threonine Kinase, cAMP-dependent protein kinase