Mcm2 minichromosome maintenance complex component 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mcm2provided by MGI
Official Full Name: minichromosome maintenance complex component 2provided by MGI
Primary source: MGI:MGI:105380
See related: Ensembl:ENSMUSG00000002870 AllianceGenome:MGI:105380
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: BM28; CDCL1; Mcmd2; mKIAA0030
Summary: Enables DNA replication origin binding activity; enzyme binding activity; and histone binding activity. Acts upstream of or within DNA unwinding involved in DNA replication; cellular response to interleukin-4; and nucleosome assembly. Located in nucleus. Part of nuclear origin of replication recognition complex. Is expressed in several structures, including central nervous system; future brain; inner cell mass; limb; and vertebral axis musculature. Human ortholog(s) of this gene implicated in Alzheimer's disease and autosomal dominant nonsyndromic deafness 70. Orthologous to human MCM2 (minichromosome maintenance complex component 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in liver E14.5 (RPKM 77.8), liver E14 (RPKM 74.1) and 21 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 D1; 6 39.64 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (88860456..88875762, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (88883474..88898780, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The importance of nuclear RAGE-Mcm2 axis in diabetes or cancer-associated replication stress.
Title: The importance of nuclear RAGE-Mcm2 axis in diabetes or cancer-associated replication stress.
Mcm2 promotes stem cell differentiation via its ability to bind H3-H4.
Title: Mcm2 promotes stem cell differentiation via its ability to bind H3-H4.
Mcm2 hypomorph leads to acute leukemia or hematopoietic stem cell failure, dependent on genetic context.
Title: Mcm2 hypomorph leads to acute leukemia or hematopoietic stem cell failure, dependent on genetic context.
Segregation of parental histones to the leading strand increased markedly in cells with histone-binding mutations in MCM2, part of the replicative helicase, exacerbating histone posttranslational modifications sister chromatid asymmetry.
Title: MCM2 promotes symmetric inheritance of modified histones during DNA replication.
MCM2 is dynamically expressed in both proliferative and differentiated stromal cells during mouse periimplantation uterus. expression of Mcm2 is induced by progesterone action in the mouse uterine stroma. siRNA-mediated silencing of MCM2 arrests the cell cycle at G1-S transition during stromal cell proliferation. downregulation of Mcm2 could also compromise stromal cell differentiation.
Title: MCM2 mediates progesterone-induced endometrial stromal cell proliferation and differentiation in mice.
MCM2 deficiency reduces DNA replication initiation in gene-rich regions of the genome.
Title: Effect of minichromosome maintenance protein 2 deficiency on the locations of DNA replication origins.
these data demonstrate that active MCM2-7 repression is a physiologically important mechanism for RS-induced cell cycle arrest and genome maintenance on an organismal level.
Title: Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation.
Hematopoietic cells originally express higher levels MCM 2 protein and undergo more frequent apoptosis following doxorubicin-induced DNA-damage in the presence of the FLV envelope protein gp70.
Title: Minichromosome maintenance 2 bound with retroviral Gp70 is localized to cytoplasm and enhances DNA-damage-induced apoptosis.
Mcm2 deficiency results in short deletions allowing high resolution identification of genes contributing to lymphoblastic lymphoma.
Title: Mcm2 deficiency results in short deletions allowing high resolution identification of genes contributing to lymphoblastic lymphoma.
gp70 enhances cellular DNA-damage-induced signaling in association with host-specific cellular proteins including acinus and MCM2 resulting in the activation of DNA-PK to phosphorylate P53.
Title: A novel role for acinus and MCM2 as host-specific signaling enhancers of DNA-damage-induced apoptosis in association with viral protein gp70.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AA959861 (e-PCR)
- UniSTS:159540

Mcm2 (e-PCR), detects polymorphism
- UniSTS:547455

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
contributes_to 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables histone binding	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
contributes_to single-stranded DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within DNA replication initiation	TAS Traceable Author Statement more info	PubMed
involved_in DNA unwinding involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within DNA unwinding involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA unwinding involved in DNA replication	ISO Inferred from Sequence Orthology more info
involved_in apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to interleukin-4	IDA Inferred from Direct Assay more info	PubMed
involved_in cochlea development	ISO Inferred from Sequence Orthology more info
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of DNA helicase activity	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within nucleosome assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in premeiotic DNA replication	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of CMG complex	ISO Inferred from Sequence Orthology more info
part_of MCM complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MCM complex	IDA Inferred from Direct Assay more info	PubMed
part_of MCM complex	ISO Inferred from Sequence Orthology more info
part_of chromatin	ISO Inferred from Sequence Orthology more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
part_of nuclear origin of replication recognition complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: DNA replication licensing factor MCM2

Names: mini chromosome maintenance deficient 2; minichromosome maintenance deficient 2 mitotin; minichromosome maintenance protein 2 homolog; nuclear protein BM28

NP_032590.2

EC 3.6.4.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008564.2 → NP_032590.2 DNA replication licensing factor MCM2

See identical proteins and their annotated locations for NP_032590.2

Status: VALIDATED

Source sequence(s)

AK129039, BY324067, CK378639

Consensus CDS

CCDS39554.1

UniProtKB/Swiss-Prot

O08971, O89057, P97310, Q8C2R0

UniProtKB/TrEMBL

Q3UJN1

Related

ENSMUSP00000061923.7, ENSMUST00000058011.8

Conserved Domains (4) summary

smart00350
Location:291 → 803

MCM; minichromosome maintenance proteins

pfam12619
Location:72 → 182

MCM2_N; Mini-chromosome maintenance protein 2

pfam14551
Location:198 → 310

MCM_N; MCM N-terminal domain

cl21455
Location:485 → 632

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases