Mastl microtubule associated serine/threonine kinase-like [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mastlprovided by MGI
Official Full Name: microtubule associated serine/threonine kinase-likeprovided by MGI
Primary source: MGI:MGI:1914371
See related: Ensembl:ENSMUSG00000026779 AllianceGenome:MGI:1914371
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: GW; GWL; THC2; MAST-L; 2700091H24Rik
Summary: Predicted to enable protein serine/threonine kinase activity. Acts upstream of or within female meiosis II and positive regulation of ubiquitin protein ligase activity. Predicted to be located in centrosome; cleavage furrow; and nucleoplasm. Predicted to be active in nucleus. Is expressed in several structures, including early conceptus; otic capsule; secondary oocyte; thymus primordium; and tooth. Human ortholog(s) of this gene implicated in thrombocytopenia. Orthologous to human MASTL (microtubule associated serine/threonine kinase like). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E11.5 (RPKM 3.1), placenta adult (RPKM 2.7) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 A3; 2 15.23 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (23006549..23046036, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (23116539..23156024, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The MASTL/PP2A cell cycle kinase-phosphatase module restrains PI3K-Akt activity in an mTORC1-dependent manner.
Title: The MASTL/PP2A cell cycle kinase-phosphatase module restrains PI3K-Akt activity in an mTORC1-dependent manner.
The Greatwall kinase safeguards the genome integrity by affecting the kinome activity in mitosis.
Title: The Greatwall kinase safeguards the genome integrity by affecting the kinome activity in mitosis.
reveal an unexpected role of Mastl in actin cytoskeletal dynamics in postmitotic cells and suggest that the thrombocytopenia-associated mutation in MASTL is a pathogenic dominant mutation that mimics decreased PP2A activity resulting in altered phosphorylation of cytoskeletal regulatory pathways.
Title: Thrombocytopenia-associated mutations in Ser/Thr kinase MASTL deregulate actin cytoskeletal dynamics in platelets.
Findings reveal the role of RSK in mouse oocytes, showing that the RSK-MASTL pathway allows mammalian-specific prolonged meiotic exit and ensures the faithful conversion from sperm to paternal pronuclei.
Title: RSK-MASTL Pathway Delays Meiotic Exit in Mouse Zygotes to Ensure Paternal Chromosome Stability.
using in vitro dephosphorylation assays, we demonstrate that Mastl promotes persistent MPS1 phosphorylation by inhibiting PP2A/B55-mediated MPS1 dephosphorylation rather than affecting Cdk1 kinase activity. Our findings establish a key regulatory function of the Greatwall kinase/Mastl - PP2A/B55 pathway in preventing premature SAC silencing
Title: Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint.
Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity.
Title: Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II.
Data show that Mastl (Greatwall)-null cells display mitotic collapse after nuclear envelope breakdown (NEB) characterized by defective chromosome condensation and prometaphase arrest.
Title: Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (2)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C88295 (e-PCR)
- UniSTS:160853

BB453705 (e-PCR)
- UniSTS:207295

MASTL (e-PCR)
- UniSTS:506363

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in G2/M transition of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within female meiosis II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of phosphoprotein phosphatase activity	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-serine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of ubiquitin protein ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell cycle	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in cleavage furrow	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine/threonine-protein kinase greatwall

Names: microtubule-associated serine/threonine-protein kinase-like

NP_080255.3

EC 2.7.11.1

XP_017174703.1

EC 2.7.11.1

XP_030107837.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_025979.4 → NP_080255.3 serine/threonine-protein kinase greatwall

See identical proteins and their annotated locations for NP_080255.3

Status: VALIDATED

Source sequence(s)

AK030140, AL845257, CD546688, CN536399

Consensus CDS

CCDS15727.1

UniProtKB/Swiss-Prot

A2AQY2, Q3MIA9, Q5RJW0, Q6NXX9, Q8BVF3, Q8C0P0, Q9CZH9, Q9D9V0

Related

ENSMUSP00000028119.7, ENSMUST00000028119.7

Conserved Domains (3) summary

smart00220
Location:34 → 194

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05610
Location:29 → 222

STKc_MASTL; Catalytic domain of the Serine/Threonine Kinase, Microtubule-associated serine/threonine-like kinase (also called greatwall kinase)

cl21453
Location:658 → 856

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

23006549..23046036 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030251977.2 → XP_030107837.1 serine/threonine-protein kinase greatwall isoform X1

Conserved Domains (1) summary

cl21453
Location:582 → 780

PKc_like; Protein Kinases, catalytic domain
XM_017319214.2 → XP_017174703.1 serine/threonine-protein kinase greatwall isoform X2

Conserved Domains (1) summary

cl21453
Location:478 → 676

PKc_like; Protein Kinases, catalytic domain