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    Rnf128 ring finger protein 128 [ Mus musculus (house mouse) ]

    Gene ID: 66889, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Rnf128provided by MGI
    Official Full Name
    ring finger protein 128provided by MGI
    Primary source
    MGI:MGI:1914139
    See related
    Ensembl:ENSMUSG00000031438 AllianceGenome:MGI:1914139
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    GRAIL; Greul1; 1300002C13Rik
    Summary
    Enables ubiquitin protein ligase activity. Acts upstream of or within negative regulation of cytokine production. Located in Golgi apparatus; endoplasmic reticulum; and late endosome. Is expressed in several structures, including alimentary system; extraembryonic component; genitourinary system; nervous system; and sensory organ. Orthologous to human RNF128 (ring finger protein 128). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in placenta adult (RPKM 42.6), small intestine adult (RPKM 42.3) and 17 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    X F1; X 61.35 cM
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) X NC_000086.8 (138464089..138573894)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) X NC_000086.7 (139563340..139673145)

    Chromosome X - NC_000086.8Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_47993 Neighboring gene transcription elongation factor B (SIII), polypeptide 2 pseudogene Neighboring gene STARR-seq mESC enhancer starr_47994 Neighboring gene STARR-seq mESC enhancer starr_47995 Neighboring gene RPA1 related single stranded DNA binding protein, X-linked Neighboring gene STARR-seq mESC enhancer starr_47996 Neighboring gene STARR-seq mESC enhancer starr_47997 Neighboring gene glyceraldehyde-3-phosphate dehydrogenase pseudogene Neighboring gene TBC1 domain family, member 8B Neighboring gene ribosomal protein S4, X-linked pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. RNF128 regulates neutrophil infiltration and myeloperoxidase functions to prevent acute lung injury. Liu PY, et al. Cell Death Dis, 2023 Jun 21. PMID 37344492, Free PMC Article
    2. How GRAIL controls Treg function to maintain self-tolerance. Fathman CG, et al. Front Immunol, 2022. PMID 36569931, Free PMC Article
    3. Macrophage expression of E3 ubiquitin ligase Grail protects mice from lipopolysaccharide-induced hyperinflammation and organ injury. Shih CC, et al. PLoS One, 2018. PMID 30571701, Free PMC Article
    4. Grail is involved in adipocyte differentiation and diet-induced obesity. Liu P, et al. Cell Death Dis, 2018 May 1. PMID 29743578, Free PMC Article
    5. Upregulation of GRAIL is associated with impaired CD4 T cell proliferation in sepsis. Aziz M, et al. J Immunol, 2014 Mar 1. PMID 24477910, Free PMC Article

    See all (55) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. GRAIL gene knockout mice protect against aging-related and noise-induced hearing loss.
      Title: GRAIL gene knockout mice protect against aging-related and noise-induced hearing loss.
    2. RNF128 regulates neutrophil infiltration and myeloperoxidase functions to prevent acute lung injury.
      Title: RNF128 regulates neutrophil infiltration and myeloperoxidase functions to prevent acute lung injury.
    3. How GRAIL controls Treg function to maintain self-tolerance.
      Title: How GRAIL controls Treg function to maintain self-tolerance.
    4. E3 ubiquitin ligase Grail promotes hepatic steatosis through Sirt1 inhibition.
      Title: E3 ubiquitin ligase Grail promotes hepatic steatosis through Sirt1 inhibition.
    5. results indicate that Grail plays a pivotal role in adipogenesis and diet-induced obesity by regulating PPARgamma activity.
      Title: Grail is involved in adipocyte differentiation and diet-induced obesity.
    6. Grail contributes to the attenuation of hyperinflammation caused by activated macrophages and prevents organ damage in endotoxemic mice.
      Title: Macrophage expression of E3 ubiquitin ligase Grail protects mice from lipopolysaccharide-induced hyperinflammation and organ injury.
    7. loss of Grail enhances anti-tumour reactivity and functionality of CD8(+) T cells.
      Title: Absence of Grail promotes CD8(+) T cell anti-tumour activity.
    8. GRAIL expression is regulated by the IL2/mTOR/Otubain-1 axis in CD4 T cells during Trypanosoma cruzi infection.
      Title: GRAIL and Otubain-1 are Related to T Cell Hyporesponsiveness during Trypanosoma cruzi Infection.
    9. this study identified RNF128 as an E3 ligase for K63-linked ubiquitination and activation of TBK1 to RNA and DNA viruses
      Title: E3 ubiquitin ligase RNF128 promotes innate antiviral immunity through K63-linked ubiquitination of TBK1.
    10. Grail plays a critical role in controlling Th2 cell development through a negative feedback loop by targeting STAT6 for degradation.
      Title: Grail controls Th2 cell development by targeting STAT6 for degradation.
    Submit: New GeneRIF Correction See all GeneRIFs (21)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin protein ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin protein ligase activity ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within negative regulation of cytokine production IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of protein stability ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in ubiquitin-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    is_active_in Golgi apparatus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in Golgi apparatus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in endoplasmic reticulum IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in late endosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in late endosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    E3 ubiquitin-protein ligase RNF128
    Names
    RING-type E3 ubiquitin transferase RNF128
    gene related to anergy in lymphocytes protein
    goliath-related E3 ubiquitin-protein ligase 1
    NP_001241690.1
    NP_075759.3

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001254761.1 → NP_001241690.1  E3 ubiquitin-protein ligase RNF128 isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses an alternate 5' terminal exon, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct N-terminus, compared to isoform 1.
      Source sequence(s)
      AK004847, AK018582, AL691424, CK380647, DV064262
      Consensus CDS
      CCDS57776.1
      UniProtKB/TrEMBL
      A2AGL8
      Related
      ENSMUSP00000108650.2, ENSMUST00000113027.8
      Conserved Domains (2) summary
      cd02122
      Location:34 → 167
      PA_GRAIL_like; PA _GRAIL_like: Protease-associated (PA) domain GRAIL-like. This group includes PA domain containing E3 (ubiquitin ligases) similar to human GRAIL (gene related to anergy in lymphocytes) protein. Proteins in this group contain a C3H2C3 RING finger. E3 ...
      pfam13639
      Location:249 → 292
      zf-RING_2; Ring finger domain

    2. NM_023270.5 → NP_075759.3  E3 ubiquitin-protein ligase RNF128 isoform 1 precursor

      See identical proteins and their annotated locations for NP_075759.3

      Status: VALIDATED

      Source sequence(s)
      AK004847, AK018582, CK380647
      Consensus CDS
      CCDS30435.1
      UniProtKB/Swiss-Prot
      Q3UJY0, Q9CVG1, Q9D304, Q9DBN3, Q9JJF8
      Related
      ENSMUSP00000108649.2, ENSMUST00000113026.2
      Conserved Domains (2) summary
      cd02122
      Location:48 → 193
      PA_GRAIL_like; PA _GRAIL_like: Protease-associated (PA) domain GRAIL-like. This group includes PA domain containing E3 (ubiquitin ligases) similar to human GRAIL (gene related to anergy in lymphocytes) protein. Proteins in this group contain a C3H2C3 RING finger. E3 ...
      pfam13639
      Location:275 → 318
      zf-RING_2; Ring finger domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000086.8 Reference GRCm39 C57BL/6J

      Range
      138464089..138573894
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9D304.1 GenPept UniProtKB/Swiss-Prot:Q9D304

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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