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    Rad18 RAD18 E3 ubiquitin protein ligase [ Mus musculus (house mouse) ]

    Gene ID: 58186, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Rad18provided by MGI
    Official Full Name
    RAD18 E3 ubiquitin protein ligaseprovided by MGI
    Primary source
    MGI:MGI:1890476
    See related
    Ensembl:ENSMUSG00000030254 AllianceGenome:MGI:1890476
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Rad18sc; 2810024C04Rik
    Summary
    Predicted to enable several functions, including Y-form DNA binding activity; identical protein binding activity; and ubiquitin protein ligase binding activity. Acts upstream of or within several processes, including negative regulation of DNA recombination; response to UV; and spermatogenesis. Located in XY body; chromatin; and nucleus. Is expressed in adrenal medulla; dorsal root ganglion; testis; trunk mesenchyme derived from neural crest; and urinary system. Orthologous to human RAD18 (RAD18 E3 ubiquitin protein ligase). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in testis adult (RPKM 6.5), liver E14 (RPKM 4.9) and 19 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    6 E3; 6 52.42 cM
    Exon count:
    15
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (112596812..112673650, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (112619851..112696691, complement)

    Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 23002 Neighboring gene BRO1 domain and CAAX motif containing pseudogene Neighboring gene STARR-seq mESC enhancer starr_17018 Neighboring gene STARR-seq mESC enhancer starr_17019 Neighboring gene STARR-seq mESC enhancer starr_17020 Neighboring gene STARR-positive B cell enhancer ABC_E8831 Neighboring gene STARR-positive B cell enhancer ABC_E11330 Neighboring gene predicted gene, 35824 Neighboring gene predicted gene, 26799

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Somatic hypermutation of immunoglobulin genes in Rad18 knockout mice. Shimizu T, et al. DNA Repair (Amst), 2017 Feb. PMID 28082021
    2. Rad18 confers hematopoietic progenitor cell DNA damage tolerance independently of the Fanconi Anemia pathway in vivo. Yang Y, et al. Nucleic Acids Res, 2016 May 19. PMID 26883629, Free PMC Article
    3. Differential Roles of Rad18 and Chk2 in Genome Maintenance and Skin Carcinogenesis Following UV Exposure. Tanoue Y, et al. J Invest Dermatol, 2018 Dec. PMID 29859927
    4. RAD18 activates the G2/M checkpoint through DNA damage signaling to maintain genome integrity after ionizing radiation exposure. Sasatani M, et al. PLoS One, 2015. PMID 25675240, Free PMC Article
    5. Rad18 is required for long-term maintenance of spermatogenesis in mouse testes. Sun J, et al. Mech Dev, 2009 Mar-Apr. PMID 19068231

    See all (43) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
      Title: Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
    2. these results demonstrate that the tumor-suppressive role of DNA polymerase Eta in UV-treated skin is Rad18 independent
      Title: Differential Roles of Rad18 and Chk2 in Genome Maintenance and Skin Carcinogenesis Following UV Exposure.
    3. Somatic hypermutation of immunoglobulin genes (SHM) in Rad18 KO mice was similar to wild-type mice, suggesting that Rad18 is dispensable for SHM.
      Title: Somatic hypermutation of immunoglobulin genes in Rad18 knockout mice.
    4. Rad18 confers DNA damage tolerance and tumor-suppression in a physiological setting.
      Title: Rad18 confers hematopoietic progenitor cell DNA damage tolerance independently of the Fanconi Anemia pathway in vivo.
    5. REV1 promote PCNA monoubiquitylation after UV radiation through interacting with ubiquitylated RAD18.
      Title: REV1 promotes PCNA monoubiquitylation through interacting with ubiquitylated RAD18.
    6. RAD18 can function as a mediator for DNA damage response signals to activate the G2/M checkpoint in order to maintain genome integrity and cell survival after IR exposure.
      Title: RAD18 activates the G2/M checkpoint through DNA damage signaling to maintain genome integrity after ionizing radiation exposure.
    7. repairs meiotic double-strand DNA breaks and and maintains meiotic sex chromosome inactivation
      Title: Meiotic functions of RAD18.
    8. RAD18 is intrinsically required for the long-term maintenance of spermatogenesis.
      Title: Rad18 is required for long-term maintenance of spermatogenesis in mouse testes.
    9. Rad18 suppresses viral infection in a non-specific fashion, probably by targeting incoming DNA.
      Title: Effect of DNA repair protein Rad18 on viral infection.
    10. RAD18 contributes to maintenance of genomic stability through postreplication repair
      Title: Enhanced genomic instability and defective postreplication repair in RAD18 knockout mouse embryonic stem cells.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (2)  1 citation
    • Endonuclease-mediated (2) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables Y-form DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables polyubiquitin modification-dependent protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein-containing complex binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables single-stranded DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA damage response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA damage response ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    involved_in DNA damage response ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within negative regulation of DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of chromosome segregation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in postreplication repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within protein autoubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    involved_in protein monoubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within response to UV IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within spermatogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of Rad6-Rad18 complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in XY body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in centrosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of chromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in male germ cell nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nuclear body ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nuclear inclusion body ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    located_in replication fork ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in site of double-strand break ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    E3 ubiquitin-protein ligase RAD18
    Names
    RAD18 homolog
    RING-type E3 ubiquitin transferase RAD18
    mRAD18Sc
    post-replication repair protein RAD18SC
    postreplication repair protein RAD18
    NP_001161202.1
    NP_001368860.1
    NP_001368861.1
    NP_001368862.1
    NP_067360.2
    XP_006506497.1
    XP_006506500.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001167730.1 → NP_001161202.1  E3 ubiquitin-protein ligase RAD18 isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AC157095, AK028505, AK030043
      Consensus CDS
      CCDS51870.1
      UniProtKB/TrEMBL
      E9Q392, Q8CDH2
      Related
      ENSMUSP00000070619.6, ENSMUST00000068487.12
      Conserved Domains (4) summary
      smart00734
      Location:201 → 224
      ZnF_Rad18; Rad18-like CCHC zinc finger
      cd00162
      Location:25 → 63
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
      TIGR00599
      Location:13 → 310
      rad18; DNA repair protein rad18
      pfam02037
      Location:248 → 282
      SAP; SAP domain

    2. NM_001381931.1 → NP_001368860.1  E3 ubiquitin-protein ligase RAD18 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC153591, AC157095
      UniProtKB/TrEMBL
      Q8CDH2
      Related
      ENSMUSP00000108807.2, ENSMUST00000113182.8
      Conserved Domains (1) summary
      TIGR00599
      Location:13 → 363
      rad18; DNA repair protein rad18

    3. NM_001381932.1 → NP_001368861.1  E3 ubiquitin-protein ligase RAD18 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC153591, AC157095
      UniProtKB/TrEMBL
      Q8CDH2
      Conserved Domains (1) summary
      TIGR00599
      Location:13 → 310
      rad18; DNA repair protein rad18

    4. NM_001381933.1 → NP_001368862.1  E3 ubiquitin-protein ligase RAD18 isoform 5

      Status: VALIDATED

      Source sequence(s)
      AC153591, AC157095
      Conserved Domains (1) summary
      TIGR00599
      Location:58 → 231
      rad18; DNA repair protein rad18

    5. NM_021385.2 → NP_067360.2  E3 ubiquitin-protein ligase RAD18 isoform 2

      See identical proteins and their annotated locations for NP_067360.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.
      Source sequence(s)
      AC157095, AK012795
      Consensus CDS
      CCDS39589.1
      UniProtKB/Swiss-Prot
      Q9CZB8, Q9QXK2
      UniProtKB/TrEMBL
      Q8CDH2
      Related
      ENSMUSP00000076341.5, ENSMUST00000077088.11
      Conserved Domains (4) summary
      smart00734
      Location:201 → 224
      ZnF_Rad18; Rad18-like CCHC zinc finger
      cd00162
      Location:25 → 63
      RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
      TIGR00599
      Location:13 → 363
      rad18; DNA repair protein rad18
      pfam02037
      Location:248 → 282
      SAP; SAP domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000072.7 Reference GRCm39 C57BL/6J

      Range
      112596812..112673650 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006506437.5 → XP_006506500.1  E3 ubiquitin-protein ligase RAD18 isoform X2

      Related
      ENSMUSP00000108805.2, ENSMUST00000113180.8
      Conserved Domains (1) summary
      TIGR00599
      Location:13 → 310
      rad18; DNA repair protein rad18

    2. XM_006506434.5 → XP_006506497.1  E3 ubiquitin-protein ligase RAD18 isoform X1

      UniProtKB/TrEMBL
      Q8CDH2
      Conserved Domains (1) summary
      TIGR00599
      Location:13 → 310
      rad18; DNA repair protein rad18
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9QXK2.2 GenPept UniProtKB/Swiss-Prot:Q9QXK2

    Gene LinkOut

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