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    Dgke diacylglycerol kinase, epsilon [ Mus musculus (house mouse) ]

    Gene ID: 56077, updated on 11-Apr-2024

    Summary

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    Official Symbol
    Dgkeprovided by MGI
    Official Full Name
    diacylglycerol kinase, epsilonprovided by MGI
    Primary source
    MGI:MGI:1889276
    See related
    Ensembl:ENSMUSG00000000276 AllianceGenome:MGI:1889276
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    DGK; DAGK6; DGK-epsilon
    Summary
    Predicted to enable diacylglycerol kinase activity. Involved in modulation of chemical synaptic transmission and phosphatidylinositol biosynthetic process. Located in glutamatergic synapse. Human ortholog(s) of this gene implicated in nephrotic syndrome type 7. Orthologous to human DGKE (diacylglycerol kinase epsilon). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in thymus adult (RPKM 19.4), cerebellum adult (RPKM 8.7) and 19 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Dgke in Genome Data Viewer
    Location:
    11 C; 11 54.34 cM
    Exon count:
    11
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (88926005..88951644, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (89035179..89060795, complement)

    Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene coilin Neighboring gene STARR-seq mESC enhancer starr_30379 Neighboring gene STARR-seq mESC enhancer starr_30380 Neighboring gene STARR-positive B cell enhancer ABC_E9837 Neighboring gene predicted gene 11496 Neighboring gene tripartite motif-containing 25 Neighboring gene STARR-positive B cell enhancer ABC_E2363 Neighboring gene STARR-positive B cell enhancer ABC_E5263 Neighboring gene STARR-positive B cell enhancer ABC_E5264 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:88919911-88920094 Neighboring gene STARR-positive B cell enhancer ABC_E1840 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:88927428-88927537 Neighboring gene STARR-seq mESC enhancer starr_30387 Neighboring gene diacylglycerol kinase, epsilon, opposite strand Neighboring gene STARR-positive B cell enhancer ABC_E7043 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:88938900-88939236 Neighboring gene STARR-positive B cell enhancer ABC_E8442 Neighboring gene predicted gene 525 Neighboring gene CapStarr-seq enhancer MGSCv37_chr11:88956596-88956973 Neighboring gene predicted gene, 22534 Neighboring gene STARR-seq mESC enhancer starr_30389 Neighboring gene predicted gene, 38759

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Diacylglycerol kinase epsilon protects against renal ischemia/reperfusion injury in mice through Krüppel-like factor 15/klotho pathway. Wang Z, et al. Ren Fail, 2022 Dec. PMID 35616094, Free PMC Article
    2. Diacylglycerol kinase ζ promotes actin cytoskeleton remodeling and mechanical forces at the B cell immune synapse. Merino-Cortés SV, et al. Sci Signal, 2020 Apr 14. PMID 32291315
    3. Diacylglycerol kinase ε deficiency preserves glucose tolerance and modulates lipid metabolism in obese mice. Mannerås-Holm L, et al. J Lipid Res, 2017 May. PMID 28246337, Free PMC Article
    4. Diacylglycerol Kinase-ε: Properties and Biological Roles. Epand RM, et al. Front Cell Dev Biol, 2016. PMID 27803897, Free PMC Article
    5. Loss of diacylglycerol kinase epsilon in mice causes endothelial distress and impairs glomerular Cox-2 and PGE2 production. Zhu J, et al. Am J Physiol Renal Physiol, 2016 May 1. PMID 26887830, Free PMC Article

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Ablation of diacylglycerol kinase epsilon promotes whitening of brown adipose tissue under high fat diet feeding.
      Title: Ablation of diacylglycerol kinase ε promotes whitening of brown adipose tissue under high fat diet feeding.
    2. Diacylglycerol kinase-epsilon is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment.
      Title: Diacylglycerol kinase-ε is S-palmitoylated on cysteine in the cytoplasmic end of its N-terminal transmembrane fragment.
    3. An intact zinc finger motif of the C1B domain is critical for stability and activity of diacylglycerol kinase-epsilon.
      Title: An intact zinc finger motif of the C1B domain is critical for stability and activity of diacylglycerol kinase-ε.
    4. Diacylglycerol kinase epsilon protects against renal ischemia/reperfusion injury in mice through Kruppel-like factor 15/klotho pathway.
      Title: Diacylglycerol kinase epsilon protects against renal ischemia/reperfusion injury in mice through Krüppel-like factor 15/klotho pathway.
    5. Loss of diacylglycerol kinase epsilon causes thrombotic microangiopathy by impairing endothelial VEGFA signaling.
      Title: Loss of diacylglycerol kinase ε causes thrombotic microangiopathy by impairing endothelial VEGFA signaling.
    6. Diacylglycerol kinase zeta promotes actin cytoskeleton remodeling and mechanical forces at the B cell immune synapse.
      Title: Diacylglycerol kinase ζ promotes actin cytoskeleton remodeling and mechanical forces at the B cell immune synapse.
    7. Mice lacking DGKepsilon show increased beige adipogenesis in visceral white adipose tissue after long-term high fat diet in a COX-2- dependent manner.
      Title: Mice lacking DGKε show increased beige adipogenesis in visceral white adipose tissue after long-term high fat diet in a COX-2- dependent manner.
    8. DGKepsilon plays a role in glucose and energy homeostasis by modulating lipid metabolism in skeletal muscle
      Title: Diacylglycerol kinase ε deficiency preserves glucose tolerance and modulates lipid metabolism in obese mice.
    9. Our results unveil an unexpected role of Dgke in the induction of cyclooxygenase-2 and in the regulation of glomerular prostanoids synthesis under stress
      Title: Loss of diacylglycerol kinase epsilon in mice causes endothelial distress and impairs glomerular Cox-2 and PGE2 production.
    10. Inhibition of lipid signaling enzyme diacylglycerol kinase epsilon attenuates mutant huntingtin toxicity.
      Title: Inhibition of lipid signaling enzyme diacylglycerol kinase epsilon attenuates mutant huntingtin toxicity.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (3)  1 citation
    • Endonuclease-mediated (4) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP-dependent diacylglycerol kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ATP-dependent diacylglycerol kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in diacylglycerol metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within lipid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in lipid phosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in modulation of chemical synaptic transmission IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in modulation of chemical synaptic transmission IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in phosphatidic acid biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in phosphatidylinositol biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein kinase C-activating G protein-coupled receptor signaling pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in signal transduction IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in glutamatergic synapse IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in glutamatergic synapse IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    is_active_in membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    diacylglycerol kinase epsilon
    Names
    DAG kinase epsilon
    diglyceride kinase epsilon
    NP_001349774.1
    NP_062378.1
    XP_011247453.1
    XP_036012756.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001362845.1 → NP_001349774.1  diacylglycerol kinase epsilon isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL646096
      Conserved Domains (4) summary
      smart00046
      Location:218 → 346
      DAGKc; Diacylglycerol kinase catalytic domain (presumed)
      cd00029
      Location:122 → 166
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      pfam00130
      Location:58 → 107
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
      cl02440
      Location:366 → 505
      DAGK_acc; Diacylglycerol kinase accessory domain

    2. NM_019505.4 → NP_062378.1  diacylglycerol kinase epsilon isoform 1

      See identical proteins and their annotated locations for NP_062378.1

      Status: VALIDATED

      Source sequence(s)
      AK049335, AK147841, AL646096, BB635763
      Consensus CDS
      CCDS25234.1
      UniProtKB/Swiss-Prot
      Q5SU69, Q9R1C6
      Related
      ENSMUSP00000000285.3, ENSMUST00000000285.9
      Conserved Domains (5) summary
      TIGR00147
      Location:216 → 526
      TIGR00147; lipid kinase, YegS/Rv2252/BmrU family
      smart00045
      Location:366 → 521
      DAGKa; Diacylglycerol kinase accessory domain (presumed)
      smart00046
      Location:218 → 346
      DAGKc; Diacylglycerol kinase catalytic domain (presumed)
      cd00029
      Location:122 → 166
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      pfam00130
      Location:58 → 107
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

    RNA

    1. NR_156181.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AK147841, AL646096

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000077.7 Reference GRCm39 C57BL/6J

      Range
      88926005..88951644 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011249151.4 → XP_011247453.1  diacylglycerol kinase epsilon isoform X2

      UniProtKB/TrEMBL
      Q8C8R9
      Conserved Domains (4) summary
      smart00046
      Location:218 → 346
      DAGKc; Diacylglycerol kinase catalytic domain (presumed)
      cd00029
      Location:122 → 166
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      pfam00130
      Location:58 → 105
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
      cl02440
      Location:366 → 505
      DAGK_acc; Diacylglycerol kinase accessory domain

    2. XM_036156863.1 → XP_036012756.1  diacylglycerol kinase epsilon isoform X1

      Conserved Domains (4) summary
      smart00045
      Location:326 → 481
      DAGKa; Diacylglycerol kinase accessory domain (presumed)
      smart00046
      Location:206 → 306
      DAGKc; Diacylglycerol kinase catalytic domain (presumed)
      cd20801
      Location:55 → 108
      C1_DGKepsilon_typeIII_rpt1; first protein kinase C conserved region 1 (C1 domain) found in type III diacylglycerol kinase, DAG kinase epsilon, and similar proteins
      cd20853
      Location:122 → 186
      C1_DGKepsilon_typeIII_rpt2; second protein kinase C conserved region 1 (C1 domain) found in type III diacylglycerol kinase, DAG kinase epsilon, and similar proteins

    RNA

    1. XR_001780032.3 RNA Sequence

    2. XR_004936996.1 RNA Sequence

    3. XR_004936995.1 RNA Sequence

    4. XR_004936997.1 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9R1C6.1 GenPept UniProtKB/Swiss-Prot:Q9R1C6

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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