Gal3st1 galactose-3-O-sulfotransferase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gal3st1provided by MGI
Official Full Name: galactose-3-O-sulfotransferase 1provided by MGI
Primary source: MGI:MGI:1858277
See related: Ensembl:ENSMUSG00000049721 AllianceGenome:MGI:1858277
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Cst; Gcst
Summary: Enables galactosylceramide sulfotransferase activity. Involved in galactosylceramide metabolic process. Acts upstream of or within galactosylceramide biosynthetic process; myelination; and spermatogenesis. Predicted to be integral component of membrane. Is expressed in brain; future spinal cord; heart ventricle; and spinal cord cervical region. Orthologous to human GAL3ST1 (galactose-3-O-sulfotransferase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in stomach adult (RPKM 105.3), duodenum adult (RPKM 57.3) and 7 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 A1; 11 2.81 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (3933636..3949328)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (3983636..3999328)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

lipidomics analysis demonstrated anticipated sulfatide mass deficiency in CST defect mouse brain and revealed novel brain lipidome homeostasis in these mice.
Title: Alterations in mouse brain lipidome after disruption of CST gene: a lipidomics study.
Data indicate that mice with cerebroside sulfotransferases (Cst) and UDP-glucose:ceramide glucosyltransferase (Ugcg)/Cst deficiency had lower ammonium excretion.
Title: Sulfatides are required for renal adaptation to chronic metabolic acidosis.
Protein-overload mice exhibited marked kidney injuries, enhancement of hepatic oxidative stress, decreased levels of serum and hepatic sulfatides, and decreased expression of hepatic cerebroside sulfotransferase.
Title: Attenuation of kidney injuries maintains serum sulfatide levels dependent on hepatic synthetic ability: a possible involvement of oxidative stress.
CST-deficient optic nerves were used to examine migration and proliferation of oligodendrocyte precursor cells.
Title: Increased numbers of oligodendrocyte lineage cells in the optic nerves of cerebroside sulfotransferase knockout mice.
These findings define transcriptional regulation of CST (Gal3st1) expression as a critical role for BORIS in spermatogenesis.
Title: Expression of a testis-specific form of Gal3st1 (CST), a gene essential for spermatogenesis, is regulated by the CTCF paralogous gene BORIS.
The number of OLs in the CST-null mice is also increased in the spinal cord and that this elevated OL population is maintained through, at least, 7 months of age.
Title: Adult CST-null mice maintain an increased number of oligodendrocytes.
Strong evidence is provided that murine cerebroside sulfotransferase localizes to the Golgi apparatus but not to the trans-Golgi network.
Title: Cerebroside sulfotransferase forms homodimers in living cells.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (2) 1 citation

General gene information

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Markers

Gal3st1 (e-PCR), detects polymorphism
- UniSTS:508004

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables galactosylceramide sulfotransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables galactosylceramide sulfotransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables galactosylceramide sulfotransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables galactosylceramide sulfotransferase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in galactosylceramide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within galactosylceramide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in galactosylceramide metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycerolipid metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within glycolipid biosynthetic process	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in myelination	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within myelination	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sphingolipid metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in Golgi membrane	TAS Traceable Author Statement more info	PubMed
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed

General protein information

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Preferred Names: galactosylceramide sulfotransferase

Names: 3'-phosphoadenosine-5'-phosphosulfate:Galcer sulfotransferase; 3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase; Galcer sulfotransferase; cerebroside sulfotransferase

NP_001171162.1

EC 2.8.2.11

NP_001171174.1

EC 2.8.2.11

NP_001369219.1

EC 2.8.2.11

NP_001369220.1

EC 2.8.2.11

NP_058618.2

EC 2.8.2.11

XP_006514819.2

EC 2.8.2.11

XP_006514820.1

EC 2.8.2.11

XP_036012743.1

EC 2.8.2.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001177691.1 → NP_001171162.1 galactosylceramide sulfotransferase

See identical proteins and their annotated locations for NP_001171162.1

Status: VALIDATED

Description

Transcript Variant: This variant (3, also known as form E) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AK002510, AK007645, AL807395, BB804195

Consensus CDS

CCDS24373.1

UniProtKB/Swiss-Prot

Q9D8V6, Q9JHE4

Related

ENSMUSP00000105608.2, ENSMUST00000109981.2

Conserved Domains (1) summary

cl22970
Location:4 → 408

Sulfotransfer_2; Sulfotransferase family
NM_001177703.1 → NP_001171174.1 galactosylceramide sulfotransferase

See identical proteins and their annotated locations for NP_001171174.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AK007645, AL807395, BB804195, CX239940

Consensus CDS

CCDS24373.1

UniProtKB/Swiss-Prot

Q9D8V6, Q9JHE4

Related

ENSMUSP00000077815.2, ENSMUST00000078757.8

Conserved Domains (1) summary

cl22970
Location:4 → 408

Sulfotransfer_2; Sulfotransferase family
NM_001382290.1 → NP_001369219.1 galactosylceramide sulfotransferase

Status: VALIDATED

Source sequence(s)

AL807395

UniProtKB/Swiss-Prot

Q9D8V6, Q9JHE4

Conserved Domains (1) summary

cl22970
Location:4 → 408

Sulfotransfer_2; Sulfotransferase family
NM_001382291.1 → NP_001369220.1 galactosylceramide sulfotransferase

Status: VALIDATED

Source sequence(s)

AL807395

UniProtKB/Swiss-Prot

Q9D8V6, Q9JHE4

Conserved Domains (1) summary

cl22970
Location:4 → 408

Sulfotransfer_2; Sulfotransferase family
NM_016922.3 → NP_058618.2 galactosylceramide sulfotransferase

See identical proteins and their annotated locations for NP_058618.2

Status: VALIDATED

Description

Transcript Variant: This variant (1, also known as form A) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. An in-frame AUG is located 29 codons upstream of the annotated translation start site but is not being annotated as a start site since it would be inhibited by an overlapping uORF with a strong Kozak sequence.

Source sequence(s)

AK007645, AL807395, BB804195, CX239940

Consensus CDS

CCDS24373.1

UniProtKB/Swiss-Prot

Q9D8V6, Q9JHE4

Related

ENSMUSP00000058348.8, ENSMUST00000063004.14

Conserved Domains (1) summary

cl22970
Location:4 → 408

Sulfotransfer_2; Sulfotransferase family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

3933636..3949328

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036156850.1 → XP_036012743.1 galactosylceramide sulfotransferase isoform X2

Conserved Domains (1) summary

cl22970
Location:28 → 437

Sulfotransfer_2; Sulfotransferase family
XM_006514757.5 → XP_006514820.1 galactosylceramide sulfotransferase isoform X1

See identical proteins and their annotated locations for XP_006514820.1

UniProtKB/Swiss-Prot

Q9D8V6, Q9JHE4

Conserved Domains (1) summary

cl22970
Location:4 → 408

Sulfotransfer_2; Sulfotransferase family
XM_006514756.5 → XP_006514819.2 galactosylceramide sulfotransferase isoform X1

See identical proteins and their annotated locations for XP_006514819.2

UniProtKB/Swiss-Prot

Q9D8V6, Q9JHE4

Conserved Domains (1) summary

cl22970
Location:4 → 408

Sulfotransfer_2; Sulfotransferase family