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    Usp2 ubiquitin specific peptidase 2 [ Mus musculus (house mouse) ]

    Gene ID: 53376, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Usp2provided by MGI
    Official Full Name
    ubiquitin specific peptidase 2provided by MGI
    Primary source
    MGI:MGI:1858178
    See related
    Ensembl:ENSMUSG00000032010 AllianceGenome:MGI:1858178
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Ubp41; B930035K21Rik
    Summary
    Enables identical protein binding activity. Involved in several processes, including circadian regulation of gene expression; entrainment of circadian clock by photoperiod; and locomotor rhythm. Located in several cellular components, including cell cortex; nucleus; and perinuclear region of cytoplasm. Is expressed in several structures, including alimentary system; central nervous system; eye; integumental system; and reproductive system. Orthologous to human USP2 (ubiquitin specific peptidase 2). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in testis adult (RPKM 55.3), heart adult (RPKM 6.4) and 5 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    9 A5.1; 9 24.53 cM
    Exon count:
    21
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 9 NC_000075.7 (43978318..44006924)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 9 NC_000075.6 (44067021..44095627)

    Chromosome 9 - NC_000075.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 39323 Neighboring gene CapStarr-seq enhancer MGSCv37_chr9:43828190-43828343 Neighboring gene CapStarr-seq enhancer MGSCv37_chr9:43851446-43851555 Neighboring gene CapStarr-seq enhancer MGSCv37_chr9:43855184-43855482 Neighboring gene thymus cell antigen 1, theta Neighboring gene STARR-positive B cell enhancer ABC_E1383 Neighboring gene predicted gene, 39325 Neighboring gene predicted gene, 38444 Neighboring gene C1q and tumor necrosis factor related protein 5 Neighboring gene STARR-seq mESC enhancer starr_23954 Neighboring gene membrane frizzled-related protein Neighboring gene ring finger protein 26

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Macrophage ubiquitin-specific protease 2 contributes to motility, hyperactivation, capacitation, and in vitro fertilization activity of mouse sperm. Hashimoto M, et al. Cell Mol Life Sci, 2021 Mar. PMID 33104844
    2. Inhibition of ubiquitin-specific protease 2 causes accumulation of reactive oxygen species, mitochondria dysfunction, and intracellular ATP decrement in C2C12 myoblasts. Hashimoto M, et al. Physiol Rep, 2019 Jul. PMID 31353872, Free PMC Article
    3. 3,3'-Diindolylmethane suppresses high-fat diet-induced obesity through inhibiting adipogenesis of pre-adipocytes by targeting USP2 activity. Yang H, et al. Mol Nutr Food Res, 2017 Oct. PMID 28586165
    4. Mice carrying ubiquitin-specific protease 2 (Usp2) gene inactivation maintain normal sodium balance and blood pressure. Pouly D, et al. Am J Physiol Renal Physiol, 2013 Jul 1. PMID 23552861
    5. Mice lacking the USP2 deubiquitinating enzyme have severe male subfertility associated with defects in fertilization and sperm motility. Bedard N, et al. Biol Reprod, 2011 Sep. PMID 21543767, Free PMC Article

    See all (48) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. USP2 inhibition prevents infection with ACE2-dependent coronaviruses in vitro and is protective against SARS-CoV-2 in mice.
      Title: USP2 inhibition prevents infection with ACE2-dependent coronaviruses in vitro and is protective against SARS-CoV-2 in mice.
    2. USP2 promotes cell proliferation and metastasis in choroidal melanoma via stabilizing Snail.
      Title: USP2 promotes cell proliferation and metastasis in choroidal melanoma via stabilizing Snail.
    3. Ubiquitin-Specific Protease 2 in the Ventromedial Hypothalamus Modifies Blood Glucose Levels by Controlling Sympathetic Nervous Activation.
      Title: Ubiquitin-Specific Protease 2 in the Ventromedial Hypothalamus Modifies Blood Glucose Levels by Controlling Sympathetic Nervous Activation.
    4. Behavioral phenotyping of mice lacking the deubiquitinase USP2.
      Title: Behavioral phenotyping of mice lacking the deubiquitinase USP2.
    5. Macrophage ubiquitin-specific protease 2 contributes to motility, hyperactivation, capacitation, and in vitro fertilization activity of mouse sperm.
      Title: Macrophage ubiquitin-specific protease 2 contributes to motility, hyperactivation, capacitation, and in vitro fertilization activity of mouse sperm.
    6. USP2 knockdown causes accumulation of reactive oxygen species, mitochondria dysfunction, and intracellular ATP decrement in C2C12 myoblasts.
      Title: Inhibition of ubiquitin-specific protease 2 causes accumulation of reactive oxygen species, mitochondria dysfunction, and intracellular ATP decrement in C2C12 myoblasts.
    7. These findings have characterized an essential role of USP2a as a potential target for treatment of metastatic cancers.
      Title: USP2a Supports Metastasis by Tuning TGF-β Signaling.
    8. 3,3'-Diindolylmethane directly inhibits USP2 deubiquitinase activity in 3T3-L1 pre-adipocytes.
      Title: 3,3'-Diindolylmethane suppresses high-fat diet-induced obesity through inhibiting adipogenesis of pre-adipocytes by targeting USP2 activity.
    9. Usp2 is required for the PTH1-34-induced proliferation of osteoblasts
      Title: PTH-Induced Osteoblast Proliferation Requires Upregulation of the Ubiquitin-Specific Peptidase 2 (Usp2) Expression.
    10. Usp2 is a clock output effector related to bodily Ca2+ homeostasis, a feature that is conserved across evolution.
      Title: USP2-45 Is a Circadian Clock Output Effector Regulating Calcium Absorption at the Post-Translational Level.
    Submit: New GeneRIF Correction See all GeneRIFs (19)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (7)  1 citation
    • Endonuclease-mediated (4) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables cyclin binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables cysteine-type deubiquitinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables cysteine-type peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin protein ligase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within cell cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in circadian behavior IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in circadian regulation of gene expression IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in entrainment of circadian clock by photoperiod IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in locomotor rhythm IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within muscle organ development IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of calcium ion transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of skeletal muscle tissue development ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of transcription by RNA polymerase II ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of mitotic cell cycle ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of skeletal muscle tissue development ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein deubiquitination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein deubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein stabilization ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within rhythmic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in centrosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in perinuclear region of cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    ubiquitin carboxyl-terminal hydrolase 2
    Names
    41 kDa ubiquitin-specific protease
    deubiquitinating enzyme 2
    ubiquitin specific protease 2
    ubiquitin thioesterase 2
    ubiquitin thiolesterase 2
    ubiquitin-specific-processing protease 2
    NP_001394747.1
    NP_001394748.1
    NP_001394749.1
    NP_001394750.1
    NP_001394751.1
    NP_001394752.1
    NP_001394753.1
    NP_001394754.1
    NP_001394755.1
    NP_001394756.1
    NP_058088.2
    NP_932759.1
    NP_932760.2
    XP_006510549.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001407818.1 → NP_001394747.1  ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-69

      Status: VALIDATED

      Source sequence(s)
      AC148328
      UniProtKB/Swiss-Prot
      O88623, Q6X4T9, Q6X4U1, Q8VD74, Q9JJ87
      Related
      ENSMUSP00000135018.2, ENSMUST00000177054.8

    2. NM_001407819.1 → NP_001394748.1  ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-69

      Status: VALIDATED

      Source sequence(s)
      AC148328
      UniProtKB/Swiss-Prot
      O88623, Q6X4T9, Q6X4U1, Q8VD74, Q9JJ87

    3. NM_001407820.1 → NP_001394749.1  ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-69

      Status: VALIDATED

      Source sequence(s)
      AC148328
      UniProtKB/Swiss-Prot
      O88623, Q6X4T9, Q6X4U1, Q8VD74, Q9JJ87

    4. NM_001407821.1 → NP_001394750.1  ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-69

      Status: VALIDATED

      Source sequence(s)
      AC148328
      UniProtKB/Swiss-Prot
      O88623, Q6X4T9, Q6X4U1, Q8VD74, Q9JJ87

    5. NM_001407822.1 → NP_001394751.1  ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-69

      Status: VALIDATED

      Source sequence(s)
      AC148328
      UniProtKB/Swiss-Prot
      O88623, Q6X4T9, Q6X4U1, Q8VD74, Q9JJ87

    6. NM_001407823.1 → NP_001394752.1  ubiquitin carboxyl-terminal hydrolase 2 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC148328
      Related
      ENSMUSP00000135482.2, ENSMUST00000176416.8

    7. NM_001407824.1 → NP_001394753.1  ubiquitin carboxyl-terminal hydrolase 2 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC148328

    8. NM_001407825.1 → NP_001394754.1  ubiquitin carboxyl-terminal hydrolase 2 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC148328

    9. NM_001407826.1 → NP_001394755.1  ubiquitin carboxyl-terminal hydrolase 2 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC148328

    10. NM_001407827.1 → NP_001394756.1  ubiquitin carboxyl-terminal hydrolase 2 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC148328

    11. NM_016808.2 → NP_058088.2  ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-69

      See identical proteins and their annotated locations for NP_058088.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 3. Variants 1 and 3 encode the same protein (isoform Usp2-69).
      Source sequence(s)
      AC148328, AY255640, BE991773, BY097860
      Consensus CDS
      CCDS23094.1
      UniProtKB/Swiss-Prot
      O88623, Q6X4T9, Q6X4U1, Q8VD74, Q9JJ87
      Related
      ENSMUSP00000110479.3, ENSMUST00000114830.9
      Conserved Domains (1) summary
      pfam00443
      Location:281 → 610
      UCH; Ubiquitin carboxyl-terminal hydrolase

    12. NM_198091.2 → NP_932759.1  ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-45

      See identical proteins and their annotated locations for NP_932759.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 3. The resulting protein (isoform Usp2-45) has a distinct N-terminus and is shorter compared to isoform Usp2-69.
      Source sequence(s)
      AC148328, AY255637, BE991773, BY237727
      Consensus CDS
      CCDS23095.1
      UniProtKB/Swiss-Prot
      O88623
      Related
      ENSMUSP00000070264.8, ENSMUST00000065461.9
      Conserved Domains (1) summary
      pfam00443
      Location:58 → 387
      UCH; Ubiquitin carboxyl-terminal hydrolase

    13. NM_198092.2 → NP_932760.2  ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-69

      See identical proteins and their annotated locations for NP_932760.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) represents the longest transcript and encodes the longer protein (isoform Usp2-69). Variants 1 and 3 encode the same protein.
      Source sequence(s)
      AC148328, AY255638, BB617020, BE991773
      Consensus CDS
      CCDS23094.1
      UniProtKB/Swiss-Prot
      O88623, Q6X4T9, Q6X4U1, Q8VD74, Q9JJ87
      Related
      ENSMUSP00000034508.8, ENSMUST00000034508.14
      Conserved Domains (1) summary
      pfam00443
      Location:281 → 610
      UCH; Ubiquitin carboxyl-terminal hydrolase

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000075.7 Reference GRCm39 C57BL/6J

      Range
      43978318..44006924
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006510486.5 → XP_006510549.1  ubiquitin carboxyl-terminal hydrolase 2 isoform X3

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O88623.3 GenPept UniProtKB/Swiss-Prot:O88623

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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