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    Chek2 checkpoint kinase 2 [ Mus musculus (house mouse) ]

    Gene ID: 50883, updated on 22-Apr-2024

    Summary

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    Official Symbol
    Chek2provided by MGI
    Official Full Name
    checkpoint kinase 2provided by MGI
    Primary source
    MGI:MGI:1355321
    See related
    Ensembl:ENSMUSG00000029521 AllianceGenome:MGI:1355321
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    CHK2; Cds1; Rad53; HUCDS1
    Summary
    Enables protein kinase activity. Acts upstream of or within cellular response to gamma radiation; peptidyl-serine phosphorylation; and signal transduction by p53 class mediator. Predicted to be located in Golgi apparatus and PML body. Predicted to be active in cytoplasm and nucleus. Predicted to colocalize with chromosome, telomeric region. Is expressed in several structures, including central nervous system; genitourinary system; and liver. Human ortholog(s) of this gene implicated in several diseases, including Li-Fraumeni syndrome 2; breast cancer (multiple); colorectal cancer; osteosarcoma; and prostate cancer. Orthologous to human CHEK2 (checkpoint kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in liver E14 (RPKM 9.9), liver E14.5 (RPKM 8.7) and 23 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    5 F; 5 53.73 cM
    Exon count:
    18
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (110987668..111022006)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (110839800..110874140)

    Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_13967 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:111239028-111239211 Neighboring gene STARR-seq mESC enhancer starr_13969 Neighboring gene ES cell cis-regulatory motif 10b Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:111268841-111269029 Neighboring gene unc-51 like kinase 1 Neighboring gene STARR-seq mESC enhancer starr_13970 Neighboring gene HscB iron-sulfur cluster co-chaperone Neighboring gene tetratricopeptide repeat domain 28 Neighboring gene STARR-seq mESC enhancer starr_13974 Neighboring gene microRNA 701 Neighboring gene STARR-seq mESC enhancer starr_13978 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:111589073-111589226 Neighboring gene STARR-positive B cell enhancer mm9_chr5:111607344-111607645 Neighboring gene predicted gene, 35628

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Cell cycle kinase CHEK2 in macrophages alleviates the inflammatory response to Staphylococcus aureus-induced pneumonia. Xie F, et al. Exp Lung Res, 2022 Feb-Mar. PMID 35075953
    2. CHK2 is essential for spindle assembly and DNA repair during the first cleavage of mouse embryos. Li XH, et al. Aging (Albany NY), 2020 Jun 2. PMID 32484784, Free PMC Article
    3. Checkpoint kinase Chk2 controls renal Cyp27b1 expression, calcitriol formation, and calcium-phosphate metabolism. Fahkri H, et al. Pflugers Arch, 2015 Sep. PMID 25319519
    4. Chk2 regulates cell cycle progression during mouse oocyte maturation and early embryo development. Dai XX, et al. Mol Cells, 2014 Feb. PMID 24598997, Free PMC Article
    5. Global phosphoproteome profiling reveals unanticipated networks responsive to cisplatin treatment of embryonic stem cells. Pines A, et al. Mol Cell Biol, 2011 Dec. PMID 22006019, Free PMC Article

    See all (122) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Chk2 Modulates Bmi1-Deficiency-Induced Renal Aging and Fibrosis via Oxidative Stress, DNA Damage, and p53/TGFbeta1-Induced Epithelial-Mesenchymal Transition.
      Title: Chk2 Modulates Bmi1-Deficiency-Induced Renal Aging and Fibrosis via Oxidative Stress, DNA Damage, and p53/TGFβ1-Induced Epithelial-Mesenchymal Transition.
    2. CHEK2 deficiency increase the response to PD-1 inhibitors by affecting the tumor immune microenvironment.
      Title: CHEK2 deficiency increase the response to PD-1 inhibitors by affecting the tumor immune microenvironment.
    3. MiR-669b-5p inhibits the Alzheimer's disease development via regulation of CHEK2 in Neuro2a APPSwe/Delta9 cells.
      Title: MiR-669b-5p inhibits the Alzheimer's disease development via regulation of CHEK2 in Neuro2a APPSwe/Δ9 cells.
    4. Checkpoint kinase 1/2 inhibition potentiates anti-tumoral immune response and sensitizes gliomas to immune checkpoint blockade.
      Title: Checkpoint kinase 1/2 inhibition potentiates anti-tumoral immune response and sensitizes gliomas to immune checkpoint blockade.
    5. Cell cycle kinase CHEK2 in macrophages alleviates the inflammatory response to Staphylococcus aureus-induced pneumonia.
      Title: Cell cycle kinase CHEK2 in macrophages alleviates the inflammatory response to Staphylococcus aureus-induced pneumonia.
    6. Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation.
      Title: Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation.
    7. SIRT1 modulates cell cycle progression by regulating CHK2 acetylation-phosphorylation.
      Title: SIRT1 modulates cell cycle progression by regulating CHK2 acetylation-phosphorylation.
    8. FadA promotes DNA damage and progression of Fusobacterium nucleatum-induced colorectal cancer through up-regulation of chk2.
      Title: FadA promotes DNA damage and progression of Fusobacterium nucleatum-induced colorectal cancer through up-regulation of chk2.
    9. CHK2 is essential for spindle assembly and DNA repair during the first cleavage of mouse embryos.
      Title: CHK2 is essential for spindle assembly and DNA repair during the first cleavage of mouse embryos.
    10. this study defines a role for Chk2 in suppressing UV-induced skin carcinogenesis
      Title: Differential Roles of Rad18 and Chk2 in Genome Maintenance and Skin Carcinogenesis Following UV Exposure.
    Submit: New GeneRIF Correction See all GeneRIFs (49)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (5)  1 citation
    • Endonuclease-mediated (5) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein kinase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein serine/threonine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein serine/threonine kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA damage checkpoint signaling ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within DNA damage response ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    involved_in DNA damage response ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in G2/M transition of mitotic cell cycle ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within apoptotic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cell cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cell division IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within cellular response to gamma radiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in double-strand break repair ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in intrinsic apoptotic signaling pathway in response to DNA damage ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic DNA damage checkpoint signaling IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within mitotic G2 phase IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic intra-S DNA damage checkpoint signaling ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in mitotic spindle assembly ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of DNA damage checkpoint ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within peptidyl-serine phosphorylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of DNA-templated transcription ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of anoikis ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of protein phosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein autophosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein phosphorylation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein stabilization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of DNA-templated transcription ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of protein catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within response to gamma radiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within signal transduction in response to DNA damage IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within signal transduction in response to DNA damage ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within thymocyte apoptotic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in Golgi apparatus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in PML body ISO
    Inferred from Sequence Orthology
    more info
    		  
    colocalizes_with chromosome, telomeric region ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    serine/threonine-protein kinase Chk2
    Names
    CHK2 checkpoint homolog
    Cds1 homolog
    Rad53 homolog
    NP_001350237.1
    NP_001412508.1
    NP_001412509.1
    NP_057890.1
    XP_006535131.3
    XP_006535133.1
    XP_006535134.3
    XP_030110520.1
    XP_036021156.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001363308.1 → NP_001350237.1  serine/threonine-protein kinase Chk2 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC121934
      Conserved Domains (1) summary
      cd14084
      Location:1 → 251
      STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

    2. NM_001425579.1 → NP_001412508.1  serine/threonine-protein kinase Chk2 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC121934

    3. NM_001425580.1 → NP_001412509.1  serine/threonine-protein kinase Chk2 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AC121934
      UniProtKB/Swiss-Prot
      Q9Z265
      UniProtKB/TrEMBL
      Q543W6

    4. NM_016681.4 → NP_057890.1  serine/threonine-protein kinase Chk2 isoform 1

      See identical proteins and their annotated locations for NP_057890.1

      Status: VALIDATED

      Source sequence(s)
      AK044913, BX512104, CJ079787
      Consensus CDS
      CCDS19533.1
      UniProtKB/Swiss-Prot
      Q9Z265
      UniProtKB/TrEMBL
      Q543W6
      Related
      ENSMUSP00000066679.2, ENSMUST00000066160.3
      Conserved Domains (2) summary
      cd00060
      Location:97 → 205
      FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
      cd14084
      Location:217 → 490
      STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

    RNA

    1. NR_189322.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC121934

    2. NR_189323.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC121934

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000071.7 Reference GRCm39 C57BL/6J

      Range
      110987668..111022006
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006535071.5 → XP_006535134.3  serine/threonine-protein kinase Chk2 isoform X4

      Conserved Domains (2) summary
      cd00060
      Location:157 → 265
      FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
      cl21453
      Location:277 → 484
      PKc_like; Protein Kinases, catalytic domain

    2. XM_006535068.5 → XP_006535131.3  serine/threonine-protein kinase Chk2 isoform X2

      Conserved Domains (2) summary
      cd00060
      Location:157 → 265
      FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
      cd14084
      Location:277 → 550
      STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

    3. XM_006535070.2 → XP_006535133.1  serine/threonine-protein kinase Chk2 isoform X3

      See identical proteins and their annotated locations for XP_006535133.1

      UniProtKB/Swiss-Prot
      Q9Z265
      UniProtKB/TrEMBL
      Q543W6
      Conserved Domains (2) summary
      cd00060
      Location:97 → 205
      FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
      cd14084
      Location:217 → 490
      STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

    4. XM_036165263.1 → XP_036021156.1  serine/threonine-protein kinase Chk2 isoform X5

      Conserved Domains (2) summary
      cd00060
      Location:97 → 205
      FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...
      cl21453
      Location:217 → 424
      PKc_like; Protein Kinases, catalytic domain

    5. XM_030254660.1 → XP_030110520.1  serine/threonine-protein kinase Chk2 isoform X6

      Conserved Domains (1) summary
      cd14084
      Location:1 → 251
      STKc_Chk2; Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2

    RNA

    1. XR_004942533.1 RNA Sequence

    2. XR_003955670.2 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9Z265.1 GenPept UniProtKB/Swiss-Prot:Q9Z265

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