Exo1 exonuclease 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Exo1provided by MGI
Official Full Name: exonuclease 1provided by MGI
Primary source: MGI:MGI:1349427
See related: Ensembl:ENSMUSG00000039748 AllianceGenome:MGI:1349427
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Msa; 5730442G03Rik
Summary: Enables chromatin binding activity. Acts upstream of or within humoral immune response mediated by circulating immunoglobulin; isotype switching; and somatic hypermutation of immunoglobulin genes. Predicted to be located in nuclear body and plasma membrane. Predicted to be active in nucleus. Is expressed in several structures, including alimentary system; brain; genitourinary system; limb segment; and nose. Orthologous to human EXO1 (exonuclease 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in CNS E11.5 (RPKM 5.1), liver E14 (RPKM 3.6) and 11 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 H4; 1 81.9 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (175708334..175738962)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (175880768..175911396)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dynamic regulation of EXO1 promotes the progression from liver fibrosis to HCC through TGF-beta1/Smad signaling feedback loop.
Title: Dynamic regulation of EXO1 promotes the progression from liver fibrosis to HCC through TGF-β1/Smad signaling feedback loop.
Role of EXO1 nuclease activity in genome maintenance, the immune response and tumor suppression in Exo1D173A mice.
Title: Role of EXO1 nuclease activity in genome maintenance, the immune response and tumor suppression in Exo1D173A mice.
Calcium-stimulated AMPK-dependent phosphorylation of Exo1 protects stressed replication forks from aberrant resection.
Title: Ca(2+)-Stimulated AMPK-Dependent Phosphorylation of Exo1 Protects Stressed Replication Forks from Aberrant Resection.
It has been found that deletion of 53BP1 can relieve several of the repair deficiencies observed in B-cells lacking BLM and Exo1, just as it does in cells lacking BRCA1.
Title: DNA repair and cell cycle checkpoint defects in a mouse model of 'BRCAness' are partially rescued by 53BP1 deletion.
mice homozygous for a point mutation (D173A) in Exo1 that eliminates its nuclease activity but retains its native conformation, shows a level of trinucleotide expansion that is intermediate between Exo1+/+ and Exo1-/- animals.
Title: MutLγ promotes repeat expansion in a Fragile X mouse model while EXO1 is protective.
like canonical DNA mismatch-repair proteins, EXO1 can restrain aberrant homologous recombination events between diverged sequence elements in the genome.
Title: EXO1 suppresses double-strand break induced homologous recombination between diverged sequences in mammalian cells.
The results establish a key role for EXO1 in modulating the severity of hypomorphic MRE11 complex mutations.
Title: EXO1 is critical for embryogenesis and the DNA damage response in mice with a hypomorphic Nbs1 allele.
Exo1-mediated HR is dispensable for stem cell function in quiescent HSC, whereas it is essential to HSC response to DNA damage processing after cell cycle entry, and its loss is not compensated by intact NHEJ
Title: Exonuclease 1 is a critical mediator of survival during DNA double strand break repair in nonquiescent hematopoietic stem and progenitor cells.
In contrast to Exo1(null/null) mice, Exo1(E109K/E109K) knockin mice retain mismatch repair activity and display normal class switch recombination and meiosis.
Title: Mammalian Exo1 encodes both structural and catalytic functions that play distinct roles in essential biological processes.
RNAi-mediated EXO1 knockdown in mouse fibroblasts directly results in an alkylation-tolerant phenotype.
Title: Exonuclease 1 (Exo1) is required for activating response to S(N)1 DNA methylating agents.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (3) 1 citation
Endonuclease-mediated (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 5'-3' DNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-3' DNA exonuclease activity	ISO Inferred from Sequence Orthology more info
enables 5'-3' exonuclease activity	ISO Inferred from Sequence Orthology more info	PubMed
enables 5'-flap endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA 5'-3' DNA exonuclease activity	ISO Inferred from Sequence Orthology more info
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables exonuclease activity	IEA Inferred from Electronic Annotation more info
enables flap endonuclease activity	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on ester bonds	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nuclease activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA 5'-3' DNA exonuclease activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA recombination	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within DNA repair	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within humoral immune response mediated by circulating immunoglobulin	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within isotype switching	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mismatch repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in mismatch repair	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within nucleobase-containing compound metabolic process	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within somatic hypermutation of immunoglobulin genes	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nuclear body	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: exonuclease 1

Names: exonuclease I; mExo1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_012012.4 → NP_036142.2 exonuclease 1

See identical proteins and their annotated locations for NP_036142.2

Status: VALIDATED

Source sequence(s)

AC122854

Consensus CDS

CCDS15551.1

UniProtKB/Swiss-Prot

Q3TLM4, Q923A5, Q9QZ11

Related

ENSMUSP00000039376.7, ENSMUST00000039725.12

Conserved Domains (3) summary

cd09908
Location:213 → 289

H3TH_EXO1; H3TH domain of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease

cd09857
Location:1 → 201

PIN_EXO1; FEN-like PIN domains of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease and homologs

pfam05110
Location:555 → 765

AF-4; AF-4 proto-oncoprotein

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

175708334..175738962

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006496862.5 → XP_006496925.1 exonuclease 1 isoform X1

See identical proteins and their annotated locations for XP_006496925.1

UniProtKB/Swiss-Prot

Q3TLM4, Q923A5, Q9QZ11

Conserved Domains (3) summary

cd09908
Location:213 → 289

H3TH_EXO1; H3TH domain of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease

cd09857
Location:1 → 201

PIN_EXO1; FEN-like PIN domains of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease and homologs

pfam05110
Location:555 → 765

AF-4; AF-4 proto-oncoprotein
XM_030254499.2 → XP_030110359.1 exonuclease 1 isoform X1

UniProtKB/Swiss-Prot

Q3TLM4, Q923A5, Q9QZ11

Conserved Domains (3) summary

cd09908
Location:213 → 289

H3TH_EXO1; H3TH domain of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease

cd09857
Location:1 → 201

PIN_EXO1; FEN-like PIN domains of Exonuclease-1, a structure-specific, divalent-metal-ion dependent, 5' nuclease and homologs

pfam05110
Location:555 → 765

AF-4; AF-4 proto-oncoprotein