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    Med14 mediator complex subunit 14 [ Mus musculus (house mouse) ]

    Gene ID: 26896, updated on 11-Apr-2024

    Summary

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    Official Symbol
    Med14provided by MGI
    Official Full Name
    mediator complex subunit 14provided by MGI
    Primary source
    MGI:MGI:1349442
    See related
    Ensembl:ENSMUSG00000064127 AllianceGenome:MGI:1349442
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    ORF1; Crsp2; Gm641; Trap170; 9930001L01Rik
    Summary
    Predicted to enable transcription coactivator activity. Acts upstream of or within stem cell population maintenance. Part of mediator complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; integumental system; and sensory organ. Orthologous to human MED14 (mediator complex subunit 14). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in thymus adult (RPKM 13.6), whole brain E14.5 (RPKM 7.6) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    X A1.1; X 7.52 cM
    Exon count:
    33
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) X NC_000086.8 (12541607..12628813, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) X NC_000086.7 (12675368..12762594, complement)

    Chromosome X - NC_000086.8Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 1810030O07 gene Neighboring gene STARR-seq mESC enhancer starr_46759 Neighboring gene predicted pseudogene 4984 Neighboring gene predicted gene, 52422 Neighboring gene STARR-seq mESC enhancer starr_46762 Neighboring gene predicted gene 14634 Neighboring gene STARR-seq mESC enhancer starr_46765 Neighboring gene STARR-seq mESC enhancer starr_46768 Neighboring gene STARR-seq mESC enhancer starr_46771 Neighboring gene STARR-seq mESC enhancer starr_46772 Neighboring gene expressed sequence AA414768 Neighboring gene peptidylprolyl isomerase A pseudogene x_132.1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Detection and cloning of an X-linked locus associated with a NotI site that is not methylated on mouse inactivated X chromosome by the RLGS-M method. Takada S, et al. Genomics, 1999 Oct 1. PMID 10512684
    2. MED14 tethers mediator to the N-terminal domain of peroxisome proliferator-activated receptor gamma and is required for full transcriptional activity and adipogenesis. Grøntved L, et al. Mol Cell Biol, 2010 May. PMID 20194623, Free PMC Article
    3. Mediator complex proximal Tail subunit MED30 is critical for Mediator core stability and cardiomyocyte transcriptional network. Tan C, et al. PLoS Genet, 2021 Sep. PMID 34506481, Free PMC Article
    4. Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis. Ge K, et al. Nature, 2002 May 30. PMID 12037571
    5. Structural and functional organization of TRAP220, the TRAP/mediator subunit that is targeted by nuclear receptors. Malik S, et al. Mol Cell Biol, 2004 Sep. PMID 15340084, Free PMC Article

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. MED14 constitutes a novel anchoring point between Mediator and the N-terminal domain of PPARgamma that is necessary for functional PPARgamma-mediated recruitment of Mediator and transactivation of PPARgamma subtype-specific target genes.
      Title: MED14 tethers mediator to the N-terminal domain of peroxisome proliferator-activated receptor gamma and is required for full transcriptional activity and adipogenesis.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Gene trapped (1) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables transcription coactivator activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transcription coregulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription coregulator activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA polymerase II preinitiation complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of transcription elongation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of transcription initiation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within somatic stem cell population maintenance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of core mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of core mediator complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mediator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of mediator complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    mediator of RNA polymerase II transcription subunit 14
    Names
    CRSP complex subunit 2
    cofactor required for Sp1 transcriptional activation subunit 2 (150 kDa)
    cofactor required for Sp1 transcriptional activation, subunit 2
    thyroid hormone receptor-associated protein complex 170 kDa component

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001048208.1 → NP_001041673.1  mediator of RNA polymerase II transcription subunit 14 isoform a

      See identical proteins and their annotated locations for NP_001041673.1

      Status: VALIDATED

      Source sequence(s)
      BC065072, BX000537, CN724751
      Consensus CDS
      CCDS40874.1
      UniProtKB/Swiss-Prot
      A2ABV5, Q3TQU3, Q6P1H5, Q9R0T1, Q9WTN9
      Related
      ENSMUSP00000094239.5, ENSMUST00000096495.11
      Conserved Domains (2) summary
      pfam03957
      Location:1052 → 1147
      Jun; Jun-like transcription factor
      pfam08638
      Location:57 → 244
      Med14; Mediator complex subunit MED14

    2. NM_012005.3 → NP_036135.3  mediator of RNA polymerase II transcription subunit 14 isoform b

      Status: VALIDATED

      Source sequence(s)
      BC065072, BX631997, CN724751
      UniProtKB/Swiss-Prot
      A2ABV5
      Related
      ENSMUST00000124053.2
      Conserved Domains (2) summary
      pfam03957
      Location:1052 → 1147
      Jun; Jun-like transcription factor
      pfam08638
      Location:57 → 244
      Med14; Mediator complex subunit MED14

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000086.8 Reference GRCm39 C57BL/6J

      Range
      12541607..12628813 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006527629.5 → XP_006527692.1  mediator of RNA polymerase II transcription subunit 14 isoform X1

      Conserved Domains (2) summary
      pfam15822
      Location:1023 → 1157
      MISS; MAPK-interacting and spindle-stabilizing protein-like
      pfam08638
      Location:57 → 242
      Med14; Mediator complex subunit MED14

    2. XM_006527630.5 → XP_006527693.1  mediator of RNA polymerase II transcription subunit 14 isoform X2

      Conserved Domains (1) summary
      pfam08638
      Location:57 → 242
      Med14; Mediator complex subunit MED14

    RNA

    1. XR_878029.4 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    A2ABV5.1 GenPept UniProtKB/Swiss-Prot:A2ABV5

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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