Tiam2 T cell lymphoma invasion and metastasis 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tiam2provided by MGI
Official Full Name: T cell lymphoma invasion and metastasis 2provided by MGI
Primary source: MGI:MGI:1344338
See related: Ensembl:ENSMUSG00000023800 AllianceGenome:MGI:1344338
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: STEF; mKIAA2016; 3000002F19Rik
Summary: Enables GTPase activator activity and guanyl-nucleotide exchange factor activity. Predicted to be involved in activation of GTPase activity; positive regulation of axonogenesis; and small GTPase mediated signal transduction. Located in cytoplasm and membrane. Is expressed in several structures, including cartilage; central nervous system; genitourinary system; liver; and respiratory system. Orthologous to human TIAM2 (TIAM Rac1 associated GEF 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in frontal lobe adult (RPKM 8.1), CNS E18 (RPKM 7.8) and 16 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A1; 17 1.99 cM

Exon count:: 37

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (3376675..3569672)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (3326313..3519397)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TIAM2S-positive microglia enhance inflammation and neurotoxicity through soluble ICAM-1-mediated immune priming.
Title: TIAM2S-positive microglia enhance inflammation and neurotoxicity through soluble ICAM-1-mediated immune priming.
TIAM2S as a novel regulator for serotonin level enhances brain plasticity and locomotion behavior.
Title: TIAM2S as a novel regulator for serotonin level enhances brain plasticity and locomotion behavior.
Tiam2 is expressed in two isoforms, the full-length (Tiam2l) and a short transcript (Tiam2s). Tiam2s expression from the t-allele is strongly increased compared to the wild-type allele. By transgenic approaches we show that Tiam2s enhances t-haplotype transmission, while Tiam2l has the opposite effect.
Title: Two isoforms of the RAC-specific guanine nucleotide exchange factor TIAM2 act oppositely on transmission ratio distortion by the mouse t-haplotype.
specificity of PDZ domain and the molecular origin
Title: Distinct ligand specificity of the Tiam1 and Tiam2 PDZ domains.
The findings suggest a new role for STEF in Focal adhesion disassembly and cell migration through microtubules-mediated mechanisms.
Title: The Rac activator STEF (Tiam2) regulates cell migration by microtubule-mediated focal adhesion disassembly.
Crystal structures of the PH-CC-Ex regions revealed a single globular domain, PHCCEx domain, comprising a conventional PH subdomain associated with an antiparallel coiled coil of CC subdomain and a novel three-helical globular Ex subdomain.
Title: The PHCCEx domain of Tiam1/2 is a novel protein- and membrane-binding module.
Results describe the purification, crystallization and X-ray data collection of the Tiam1 and Tiam2 PH-CC-Ex regions.
Title: Crystallographic characterization of the membrane-targeting domains of the Rac-specific guanine nucleotide-exchange factors Tiam1 and Tiam2.
STEF is an activator of cortical neuronal migration.
Title: The in vivo roles of STEF/Tiam1, Rac1 and JNK in cortical neuronal migration.
Phosphorylation of STEF by Rho-kinase exerts the inhibitory effect on the function of STEF.
Title: Rho-kinase modulates the function of STEF, a Rac GEF, through its phosphorylation.
Extracellular and intracellular information is integrated by STEF to modulate the balance of Rho GTPase activities in the growth cone and control growth cone behavior.
Title: Roles of STEF/Tiam1, guanine nucleotide exchange factors for Rac1, in regulation of growth cone morphology.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

fi36g02.x1 (e-PCR)
- UniSTS:197388

D17Mit267 (e-PCR), detects polymorphism
- UniSTS:142138

AI429207 (e-PCR)
- UniSTS:160612

D17Brg11 (e-PCR), detects polymorphism
- UniSTS:259208

D17Brg18 (e-PCR), detects polymorphism
- UniSTS:259215

D17Brg19 (e-PCR), detects polymorphism
- UniSTS:259216

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in activation of GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of axonogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in small GTPase-mediated signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: rho guanine nucleotide exchange factor TIAM2

Names: RAS-related C3 botulinum substrate 1, guanine nucleotide exchange factor 1; SIF and TIAM1-like exchange factor; T-cell lymphoma invasion and metastasis 2; RAS-related C3 botulinum substrate 1, guanine nucleotide exchange factor 1; T-lymphoma invasion and metastasis-inducing protein 2; TIAM-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001122998.1 → NP_001116470.1 rho guanine nucleotide exchange factor TIAM2 isoform 1

See identical proteins and their annotated locations for NP_001116470.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) has an alternate 5' UTR exon, compared to variant 1. Variants 1 and 2 encode the same isoform (1).

Source sequence(s)

AK129474, BB084682, BC067048, BC079600

Consensus CDS

CCDS37421.1

UniProtKB/Swiss-Prot

Q3TSM6, Q3TZ33, Q6AXF2, Q6NXJ2, Q6ZPF3, Q9JLY2, Q9WVS3

UniProtKB/TrEMBL

A0A2X0SSQ6

Related

ENSMUSP00000125842.2, ENSMUST00000169838.9

Conserved Domains (6) summary

smart00455
Location:832 → 902

RBD; Raf-like Ras-binding domain

smart00325
Location:1124 → 1313

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd00992
Location:909 → 994

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd01230
Location:503 → 629

PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain

cd01255
Location:1315 → 1487

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain

pfam18385
Location:643 → 740

Tiam_CC_Ex; T-lymphoma invasion and metastasis CC-Ex domain
NM_001286757.1 → NP_001273686.1 rho guanine nucleotide exchange factor TIAM2 isoform 2

See identical proteins and their annotated locations for NP_001273686.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AK161947, BB084682

UniProtKB/Swiss-Prot

Q6ZPF3

Related

ENSMUST00000226913.2

Conserved Domains (2) summary

smart00325
Location:28 → 217

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd01255
Location:219 → 391

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
NM_001286758.1 → NP_001273687.1 rho guanine nucleotide exchange factor TIAM2 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AK158489, AK161947, BB084682

UniProtKB/Swiss-Prot

Q6ZPF3

Related

ENSMUST00000226997.2

Conserved Domains (2) summary

cd01255
Location:155 → 327

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain

pfam00621
Location:1 → 153

RhoGEF; RhoGEF domain
NM_011878.2 → NP_036008.2 rho guanine nucleotide exchange factor TIAM2 isoform 1

See identical proteins and their annotated locations for NP_036008.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) and variant 2 encode the same isoform (1).

Source sequence(s)

AC122350, AK129474, BB084682, BC079600

Consensus CDS

CCDS37421.1

UniProtKB/Swiss-Prot

Q3TSM6, Q3TZ33, Q6AXF2, Q6NXJ2, Q6ZPF3, Q9JLY2, Q9WVS3

UniProtKB/TrEMBL

A0A2X0SSQ6

Related

ENSMUSP00000072020.7, ENSMUST00000072156.7

Conserved Domains (6) summary

smart00455
Location:832 → 902

RBD; Raf-like Ras-binding domain

smart00325
Location:1124 → 1313

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd00992
Location:909 → 994

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd01230
Location:503 → 629

PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain

cd01255
Location:1315 → 1487

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain

pfam18385
Location:643 → 740

Tiam_CC_Ex; T-lymphoma invasion and metastasis CC-Ex domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

3376675..3569672

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006523225.5 → XP_006523288.1 rho guanine nucleotide exchange factor TIAM2 isoform X1

See identical proteins and their annotated locations for XP_006523288.1

UniProtKB/Swiss-Prot

Q3TSM6, Q3TZ33, Q6AXF2, Q6NXJ2, Q6ZPF3, Q9JLY2, Q9WVS3

UniProtKB/TrEMBL

A0A2X0SSQ6

Conserved Domains (6) summary

smart00455
Location:832 → 902

RBD; Raf-like Ras-binding domain

smart00325
Location:1124 → 1313

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd00992
Location:909 → 994

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd01230
Location:503 → 629

PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain

cd01255
Location:1315 → 1487

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain

pfam18385
Location:643 → 740

Tiam_CC_Ex; T-lymphoma invasion and metastasis CC-Ex domain
XM_006523227.5 → XP_006523290.1 rho guanine nucleotide exchange factor TIAM2 isoform X2

Conserved Domains (4) summary

smart00455
Location:832 → 902

RBD; Raf-like Ras-binding domain

cd00992
Location:909 → 994

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd01230
Location:503 → 629

PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain

pfam18385
Location:643 → 740

Tiam_CC_Ex; T-lymphoma invasion and metastasis CC-Ex domain
XM_006523226.1 → XP_006523289.1 rho guanine nucleotide exchange factor TIAM2 isoform X1

See identical proteins and their annotated locations for XP_006523289.1

UniProtKB/Swiss-Prot

Q3TSM6, Q3TZ33, Q6AXF2, Q6NXJ2, Q6ZPF3, Q9JLY2, Q9WVS3

UniProtKB/TrEMBL

A0A2X0SSQ6

Conserved Domains (6) summary

smart00455
Location:832 → 902

RBD; Raf-like Ras-binding domain

smart00325
Location:1124 → 1313

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd00992
Location:909 → 994

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd01230
Location:503 → 629

PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain

cd01255
Location:1315 → 1487

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain

pfam18385
Location:643 → 740

Tiam_CC_Ex; T-lymphoma invasion and metastasis CC-Ex domain
XM_030249767.2 → XP_030105627.1 rho guanine nucleotide exchange factor TIAM2 isoform X1

UniProtKB/Swiss-Prot

Q3TSM6, Q3TZ33, Q6AXF2, Q6NXJ2, Q6ZPF3, Q9JLY2, Q9WVS3

UniProtKB/TrEMBL

A0A2X0SSQ6

Conserved Domains (6) summary

smart00455
Location:832 → 902

RBD; Raf-like Ras-binding domain

smart00325
Location:1124 → 1313

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd00992
Location:909 → 994

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd01230
Location:503 → 629

PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain

cd01255
Location:1315 → 1487

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain

pfam18385
Location:643 → 740

Tiam_CC_Ex; T-lymphoma invasion and metastasis CC-Ex domain
XM_006523230.5 → XP_006523293.1 rho guanine nucleotide exchange factor TIAM2 isoform X4

Conserved Domains (2) summary

smart00325
Location:53 → 242

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd01255
Location:244 → 416

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
XM_006523231.4 → XP_006523294.1 rho guanine nucleotide exchange factor TIAM2 isoform X5

Conserved Domains (2) summary

smart00325
Location:51 → 240

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd01255
Location:242 → 414

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
XM_036160586.1 → XP_036016479.1 rho guanine nucleotide exchange factor TIAM2 isoform X6

Conserved Domains (2) summary

smart00325
Location:28 → 217

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd01255
Location:219 → 391

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
XM_006523229.5 → XP_006523292.1 rho guanine nucleotide exchange factor TIAM2 isoform X3

Conserved Domains (2) summary

smart00325
Location:124 → 313

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd01255
Location:315 → 487

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
XM_006523233.3 → XP_006523296.1 rho guanine nucleotide exchange factor TIAM2 isoform X6

See identical proteins and their annotated locations for XP_006523296.1

UniProtKB/Swiss-Prot

Q6ZPF3

Conserved Domains (2) summary

smart00325
Location:28 → 217

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd01255
Location:219 → 391

PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain