Car14 carbonic anhydrase 14 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Car14provided by MGI
Official Full Name: carbonic anhydrase 14provided by MGI
Primary source: MGI:MGI:1344341
See related: Ensembl:ENSMUSG00000038526 AllianceGenome:MGI:1344341
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ca14; CA-XIV
Summary: Enables carbonate dehydratase activity. Predicted to be involved in one-carbon metabolic process. Predicted to act upstream of or within carbon dioxide transport and regulation of pH. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Is expressed in several structures, including central nervous system; cranium; epiblast; eye; and urinary system. Orthologous to human CA14 (carbonic anhydrase 14). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in kidney adult (RPKM 55.3), heart adult (RPKM 42.4) and 12 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 F2.1; 3 41.48 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (95805080..95812443, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (95897768..95905117, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Carbonic anhydrase 14 protects the liver against the cytotoxicity of bile acids in a biliary bicarbonate umbrella-related manner.
Title: Carbonic anhydrase 14 protects the liver against the cytotoxicity of bile acids in a biliary bicarbonate umbrella-related manner.
Loss of luminal carbonic anhydrase XIV results in decreased biliary bicarbonate output, liver fibrosis, and cholangiocyte proliferation in mice.
Title: Loss of luminal carbonic anhydrase XIV results in decreased biliary bicarbonate output, liver fibrosis, and cholangiocyte proliferation in mice.
During inner ear development, transcripts of four cytosolic isozymes (Car1, Car2, Car3, and Car13), two membrane-bound isozymes (Car12 and Car14), and two CA-related proteins (Car8 and Car11) were expressed at higher levels than other isozymes.
Title: A systematic survey of carbonic anhydrase mRNA expression during mammalian inner ear development.
CAXIV functionally interacts with AE3, forming an extracellular membrane protein complex involved in the regulation of bicarbonate metabolism and pHi in the heart.
Title: Carbonic anhydrase XIV in the normal and hypertrophic myocardium.
Association of AE3 and CAXIV may represent a mechanism to enhance disposal of waste CO(2) and to balance pH in excitable tissues.
Title: Bicarbonate homeostasis in excitable tissues: role of AE3 Cl-/HCO3- exchanger and carbonic anhydrase XIV interaction.
CA XIV (along with carbonic anhydrase 4) plays an important role in the regulation of intracellular pH in hippocampus, via facilitation of anion exchanger AE3-mediated chloride-bicarbonate antiporter HCO3 exchange.
Title: Carbonic anhydrases CA4 and CA14 both enhance AE3-mediated Cl--HCO3- exchange in hippocampal neurons.
CA XIV is expressed in sarcoplasmic reticulum and sarcolemma of skeletal muscle.
Title: Carbonic anhydrase XIV in skeletal muscle: subcellular localization and function from wild-type and knockout mice.
The presence of CA XIV in the hepatocyte plasma membrane places it at a strategic site to control pH regulation and ion transport between the hepatocytes, sinusoids and bile canaliculi.
Title: The plasma membrane carbonic anhydrase in murine hepatocytes identified as isozyme XIV.
studies on hippocampal slices on these KO mice showed that either CA can mediate buffering after synaptic transmission in hippocampal slices in the absence of the other, but that eliminating both is as effective in blocking the buffering seen in WT mice
Title: Carbonic anhydrase IV and XIV knockout mice: roles of the respective carbonic anhydrases in buffering the extracellular space in brain.
carbonic anhydrase XIV has a role in selective inhibition of membrane-associated isozymes
Title: Expression, assay, and structure of the extracellular domain of murine carbonic anhydrase XIV: implications for selective inhibition of membrane-associated isozymes.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (3) 1 citation
Endonuclease-mediated (2)

General gene information

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Markers

Car14 (e-PCR), detects polymorphism
- UniSTS:506769

AI173904 (e-PCR)
- UniSTS:207536

AB005450 (e-PCR)
- UniSTS:163316

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables carbonate dehydratase activity	IBA Inferred from Biological aspect of Ancestor more info
enables carbonate dehydratase activity	IDA Inferred from Direct Assay more info	PubMed
enables carbonate dehydratase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables lyase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within carbon dioxide transport	IC Inferred by Curator more info	PubMed
involved_in one-carbon metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of pH	IC Inferred by Curator more info	PubMed

Component	Evidence Code	Pubs
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: carbonic anhydrase 14

Names: CA XIV; carbonate dehydratase XIV; carbonic anhydrase XIV

NP_001342679.1

EC 4.2.1.1

NP_001342680.1

EC 4.2.1.1

NP_001342681.1

EC 4.2.1.1

NP_035927.1

EC 4.2.1.1

XP_006501505.1

EC 4.2.1.1

XP_006501506.1

EC 4.2.1.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001355750.1 → NP_001342679.1 carbonic anhydrase 14 isoform 2 precursor

Status: VALIDATED

Source sequence(s)

AC092855

Conserved Domains (1) summary

cd03126
Location:29 → 278

alpha_CA_XII_XIV; Carbonic anhydrase alpha, isozymes XII and XIV. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, ...
NM_001355751.1 → NP_001342680.1 carbonic anhydrase 14 isoform 3

Status: VALIDATED

Source sequence(s)

AC092855

Conserved Domains (1) summary

cd03126
Location:20 → 269

alpha_CA_XII_XIV; Carbonic anhydrase alpha, isozymes XII and XIV. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, ...
NM_001355752.1 → NP_001342681.1 carbonic anhydrase 14 isoform 4 precursor

Status: VALIDATED

Source sequence(s)

AC092855, AK009805

UniProtKB/TrEMBL

Q8CEY0
NM_011797.3 → NP_035927.1 carbonic anhydrase 14 isoform 1 precursor

See identical proteins and their annotated locations for NP_035927.1

Status: VALIDATED

Source sequence(s)

AC092855

Consensus CDS

CCDS17625.1

UniProtKB/Swiss-Prot

Q9WVT6

Related

ENSMUSP00000036983.9, ENSMUST00000036181.15

Conserved Domains (1) summary

cd03126
Location:29 → 278

alpha_CA_XII_XIV; Carbonic anhydrase alpha, isozymes XII and XIV. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

95805080..95812443 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006501442.5 → XP_006501505.1 carbonic anhydrase 14 isoform X1

Conserved Domains (1) summary

cd03126
Location:29 → 278

alpha_CA_XII_XIV; Carbonic anhydrase alpha, isozymes XII and XIV. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, ...
XM_006501443.5 → XP_006501506.1 carbonic anhydrase 14 isoform X2

Conserved Domains (1) summary

cd03126
Location:20 → 269

alpha_CA_XII_XIV; Carbonic anhydrase alpha, isozymes XII and XIV. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, ...