Pias3 protein inhibitor of activated STAT 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pias3provided by MGI
Official Full Name: protein inhibitor of activated STAT 3provided by MGI
Primary source: MGI:MGI:1913126
See related: Ensembl:ENSMUSG00000028101 AllianceGenome:MGI:1913126
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pias3l
Summary: Enables SUMO transferase activity. Acts upstream of or within several processes, including TNFSF11-mediated signaling pathway; negative regulation of macromolecule metabolic process; and negative regulation of osteoclast differentiation. Located in cytoplasm and nucleus. Is expressed in several structures, including central nervous system; early conceptus; eye; genitourinary system; and maxillary process. Orthologous to human PIAS3 (protein inhibitor of activated STAT 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in limb E14.5 (RPKM 15.6), ovary adult (RPKM 8.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 F2.1; 3 41.95 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (96603679..96613386)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (96696361..96706070)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cancer exosome-derived miR-9 and miR-181a promote the development of early-stage MDSCs via interfering with SOCS3 and PIAS3 respectively in breast cancer.
Title: Cancer exosome-derived miR-9 and miR-181a promote the development of early-stage MDSCs via interfering with SOCS3 and PIAS3 respectively in breast cancer.
PIAS3 could have therapeutic benefits in patients with Spondyloarthritis.
Title: Protein inhibitor of activated STAT3 reduces peripheral arthritis and gut inflammation and regulates the Th17/Treg cell imbalance via STAT3 signaling in a mouse model of spondyloarthritis.
PIAS3-mediated feedback loops control cell proliferation and function as robust driving forces for Colitis-associated colorectal cancer (CAC) progression.
Title: PIAS3-mediated feedback loops promote chronic colitis-associated malignant transformation.
Results show that PIAS3 levels are reduced in a mouse model of atherosclerotic lesions and that its expression decreases in conjunction with increases in interleukin-6 expression and disease severity. These findings provide evidence that results indicate that PIAS3 is a critical repressor of atherosclerosis progression.
Title: Protein Inhibitor of Activated STAT3 Suppresses Oxidized LDL-induced Cell Responses during Atherosclerosis in Apolipoprotein E-deficient Mice.
Data indicate that PIAS3 (protein inhibitor of activated STAT3) interaction modulates EKLF (erythroid Kruppel-like factor) activity in a promoter-dependent and SUMO-independent manner.
Title: Transcriptional activity of erythroid Kruppel-like factor (EKLF/KLF1) modulated by PIAS3 (protein inhibitor of activated STAT3).
PIAS3 suppresses acute graft-versus-host disease by modulating effector T and B cell subsets through inhibition of STAT3 activation.
Title: PIAS3 suppresses acute graft-versus-host disease by modulating effector T and B cell subsets through inhibition of STAT3 activation.
These data indicate that tachypacing decreased ATBF1, leading to enhanced STAT3 DNA-binding activity due to the reduced formation of a binary complex of ATBF1 and PIAS3.
Title: Down-regulation of ATBF1 activates STAT3 signaling via PIAS3 in pacing-induced HL-1 atrial myocytes.
MRL/lpr mice have significantly increased expressions of STAT3 mRNA and protein and decreased expression of mRNA PIAS3 in the kidneys compared with BALB/C mice.
Title: [Expression of STAT3 and PIAS3 in renal tissues of MRL/lpr mice].
L97A, R99N and R99Q mutations of the PINIT domain (PIAS3(85-272) ) were found to abrogate binding to STAT3, suggesting that these residues were part of a potential binding surface.
Title: The PINIT domain of PIAS3: structure-function analysis of its interaction with STAT3.
Our results indicate that Pias3-dependent SUMOylation of photoreceptor-specific transcription factors is a common mechanism that controls both rod and cone photoreceptor subtype specification, regulating distinct molecular targets in the two cell types
Title: Pias3-dependent SUMOylation controls mammalian cone photoreceptor differentiation.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SUMO ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables SUMO transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables SUMO transferase activity	ISO Inferred from Sequence Orthology more info
enables ionotropic glutamate receptor binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables potassium channel regulator activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coregulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables transmembrane transporter binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of osteoclast differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of osteoclast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein sumoylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of membrane potential	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein sumoylation	ISO Inferred from Sequence Orthology more info
involved_in protein sumoylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein sumoylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of tumor necrosis factor-mediated signaling pathway	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: E3 SUMO-protein ligase PIAS3

Names: E3 SUMO-protein transferase PIAS3; protein inhibitor of activated STAT protein 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001165949.1 → NP_001159421.1 E3 SUMO-protein ligase PIAS3 isoform 3

See identical proteins and their annotated locations for NP_001159421.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and uses an alternate translational start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is longer than isoform 1.

Source sequence(s)

BB042656, BC051252, BE989927, BY154092

Consensus CDS

CCDS51007.1

UniProtKB/Swiss-Prot

O54714, Q80WF8, Q8R598

Related

ENSMUSP00000069259.10, ENSMUST00000064900.16

Conserved Domains (3) summary

smart00513
Location:11 → 36

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

pfam02891
Location:323 → 371

zf-MIZ; MIZ/SP-RING zinc finger

pfam14324
Location:126 → 263

PINIT; PINIT domain
NM_018812.2 → NP_061282.2 E3 SUMO-protein ligase PIAS3 isoform 2

See identical proteins and their annotated locations for NP_061282.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate translational start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.

Source sequence(s)

BB042656, BC050988, BE989927, BY154092

Consensus CDS

CCDS17645.2

UniProtKB/Swiss-Prot

O54714

Related

ENSMUSP00000102692.4, ENSMUST00000107077.4

Conserved Domains (3) summary

smart00513
Location:11 → 36

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

pfam02891
Location:288 → 336

zf-MIZ; MIZ/SP-RING zinc finger

pfam14324
Location:91 → 228

PINIT; PINIT domain
NM_146135.2 → NP_666247.1 E3 SUMO-protein ligase PIAS3 isoform 1

See identical proteins and their annotated locations for NP_666247.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes isoform 1.

Source sequence(s)

AK040619, BB042656, BE989927, BY002850

Consensus CDS

CCDS38559.1

UniProtKB/Swiss-Prot

O54714

Related

ENSMUSP00000102691.4, ENSMUST00000107076.10

Conserved Domains (3) summary

smart00513
Location:2 → 27

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:315 → 362

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:119 → 263

PINIT; PINIT domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

96603679..96613386

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030252586.2 → XP_030108446.1 E3 SUMO-protein ligase PIAS3 isoform X2

Conserved Domains (3) summary

smart00513
Location:2 → 27

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:280 → 327

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:84 → 228

PINIT; PINIT domain
XM_006501393.2 → XP_006501456.1 E3 SUMO-protein ligase PIAS3 isoform X1

See identical proteins and their annotated locations for XP_006501456.1

UniProtKB/Swiss-Prot

O54714

Conserved Domains (3) summary

smart00513
Location:2 → 27

SAP; Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation

cd16790
Location:315 → 362

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins

pfam14324
Location:119 → 263

PINIT; PINIT domain
XM_036163073.1 → XP_036018966.1 E3 SUMO-protein ligase PIAS3 isoform X3

Conserved Domains (1) summary

cd16790
Location:1 → 46

SP-RING_PIAS; SP-RING finger found in protein inhibitor of activated signal transducer and activator of transcription (PIAS) proteins