Xrcc5 X-ray repair complementing defective repair in Chinese hamster cells 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Xrcc5provided by MGI
Official Full Name: X-ray repair complementing defective repair in Chinese hamster cells 5provided by MGI
Primary source: MGI:MGI:104517
See related: Ensembl:ENSMUSG00000026187 AllianceGenome:MGI:104517
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ku80; Ku86; CTC85; CTCBF
Summary: Predicted to enable several functions, including nucleic acid binding activity; protein C-terminus binding activity; and ubiquitin protein ligase binding activity. Predicted to contribute to 5'-deoxyribose-5-phosphate lyase activity and double-stranded telomeric DNA binding activity. Acts upstream of or within several processes, including cellular response to leukemia inhibitory factor; hematopoietic stem cell differentiation; and positive regulation of neurogenesis. Located in cytoplasm and nucleus. Is expressed in thymus primordium. Human ortholog(s) of this gene implicated in chronic obstructive pulmonary disease and multiple myeloma. Orthologous to human XRCC5 (X-ray repair cross complementing 5). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E11.5 (RPKM 10.7), placenta adult (RPKM 9.8) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 C3; 1 36.5 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (72346576..72434112)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (72307417..72394953)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Xrcc5/KU80 is not required for the survival or activation of prophase-arrested oocytes in primordial follicles.
Title: Xrcc5/KU80 is not required for the survival or activation of prophase-arrested oocytes in primordial follicles.
Xrcc5/Ku80 is required for the repair of DNA damage in fully grown meiotically arrested mammalian oocytes.
Title: Xrcc5/Ku80 is required for the repair of DNA damage in fully grown meiotically arrested mammalian oocytes.
DCLK1 promotes colorectal cancer stemness and aggressiveness via the XRCC5/COX2 axis.
Title: DCLK1 promotes colorectal cancer stemness and aggressiveness via the XRCC5/COX2 axis.
Characterization of Double-Strand Break Repair Protein Ku80 Location Within the Murine Retina.
Title: Characterization of Double-Strand Break Repair Protein Ku80 Location Within the Murine Retina.
Diminished or inversed dose-rate effect on clonogenic ability in Ku-deficient rodent cells.
Title: Diminished or inversed dose-rate effect on clonogenic ability in Ku-deficient rodent cells.
C-Terminal Extensions of Ku70 and Ku80 Differentially Influence DNA End Binding Properties.
Title: C-Terminal Extensions of Ku70 and Ku80 Differentially Influence DNA End Binding Properties.
RT-PCR and immunobloting data show that inhibitory effect of sodium butyrate takes place at the level of Rad51 and XRCC5 gene transcription and the content of Rad51 and Ku80 proteins.
Title: INFLUENCE OF HDAC INHIBITOR SODIUM BUTYRATE ON THE EXPRESSION OF DNA REPAIR GENES Rad51 AND XRCC5 IN mEras-Waf1+/+ AND mEras-Waf1–/–.
Ku (ku70/ku80 heterodimer)roles in nonhomologous end joining and base excision repair pathways of DNA repair are overviewed.
Title: [The role of Ku antigen in the repair of apurinic/apyrimidinic sites in DNA].
the downregulation of Ku80 and an impairment of repair activity in squamous cells, which are mediated by miR-31.
Title: K14-EGFP-miR-31 transgenic mice have high susceptibility to chemical-induced squamous cell tumorigenesis that is associating with Ku80 repression.
Inactivation of Ku80 and DNA-PKCS causes reduced lifespan and bodyweight.
Title: The progeroid phenotype of Ku80 deficiency is dominant over DNA-PKCS deficiency.

Submit: New GeneRIF Correction See all GeneRIFs (39)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Chemically induced (ENU) (1)
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC17958P1 (e-PCR)
- UniSTS:271661

RH118241 (e-PCR)
- UniSTS:160916

Xrcc5 (e-PCR), detects polymorphism
- UniSTS:144802

AI314015 (e-PCR)
- UniSTS:178411

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
contributes_to 5'-deoxyribose-5-phosphate lyase activity	ISO Inferred from Sequence Orthology more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	ISO Inferred from Sequence Orthology more info
enables ATP-dependent activity, acting on DNA	ISO Inferred from Sequence Orthology more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to DNA end binding	ISO Inferred from Sequence Orthology more info
enables DNA end binding	ISO Inferred from Sequence Orthology more info
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables RNA binding	ISO Inferred from Sequence Orthology more info
enables U3 snoRNA binding	ISO Inferred from Sequence Orthology more info
enables damaged DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to double-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
contributes_to double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
contributes_to double-stranded telomeric DNA binding	ISO Inferred from Sequence Orthology more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables telomeric DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables telomeric DNA binding	ISO Inferred from Sequence Orthology more info
enables transcription cis-regulatory region binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA repair	IEA Inferred from Electronic Annotation more info
involved_in activation of innate immune response	ISO Inferred from Sequence Orthology more info
involved_in cellular hyperosmotic salinity response	ISO Inferred from Sequence Orthology more info
involved_in cellular response to X-ray	ISO Inferred from Sequence Orthology more info
involved_in cellular response to gamma radiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within double-strand break repair	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair	ISO Inferred from Sequence Orthology more info
involved_in double-strand break repair via nonhomologous end joining	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via nonhomologous end joining	ISO Inferred from Sequence Orthology more info
involved_in double-strand break repair via nonhomologous end joining	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within hematopoietic stem cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hematopoietic stem cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within innate immune response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within leptotene	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of t-circle formation	ISO Inferred from Sequence Orthology more info
acts_upstream_of neurogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of neurogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein kinase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of telomere maintenance via telomerase	ISO Inferred from Sequence Orthology more info
involved_in recombinational repair	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within ribosome biogenesis	IEA Inferred from Electronic Annotation more info
involved_in small-subunit processome assembly	ISO Inferred from Sequence Orthology more info
involved_in telomere maintenance	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of DNA-dependent protein kinase complex	ISO Inferred from Sequence Orthology more info
part_of DNA-dependent protein kinase-DNA ligase 4 complex	ISO Inferred from Sequence Orthology more info
part_of Ku70:Ku80 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Ku70:Ku80 complex	ISO Inferred from Sequence Orthology more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
part_of nonhomologous end joining complex	ISO Inferred from Sequence Orthology more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein-DNA complex	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of ribonucleoprotein complex	ISO Inferred from Sequence Orthology more info
located_in site of DNA damage	ISO Inferred from Sequence Orthology more info
part_of small-subunit processome	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: X-ray repair cross-complementing protein 5

Names: ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; DNA repair protein XRCC5; Ku p80; ku autoantigen protein p86 homolog; nuclear factor IV

NP_001344448.1

EC 3.6.4.-

NP_001344449.1

EC 3.6.4.-

NP_033559.2

EC 3.6.4.-

XP_006495955.1

EC 3.6.4.-

XP_006495956.1

EC 3.6.4.-

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001357519.1 → NP_001344448.1 X-ray repair cross-complementing protein 5 isoform 2

Status: VALIDATED

Source sequence(s)

AC114629, AC114905

Conserved Domains (3) summary

cd00873
Location:225 → 523

KU80; Ku-core domain, Ku80 subfamily; Ku80 is a subunit of the Ku protein, which plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination. The mechanism underlying the ...

pfam03731
Location:1 → 225

Ku_N; Ku70/Ku80 N-terminal alpha/beta domain

pfam08785
Location:575 → 687

Ku_PK_bind; Ku C terminal domain like
NM_001357520.1 → NP_001344449.1 X-ray repair cross-complementing protein 5 isoform 2

Status: VALIDATED

Source sequence(s)

AC114629, AC114905

Conserved Domains (3) summary

cd00873
Location:225 → 523

KU80; Ku-core domain, Ku80 subfamily; Ku80 is a subunit of the Ku protein, which plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination. The mechanism underlying the ...

pfam03731
Location:1 → 225

Ku_N; Ku70/Ku80 N-terminal alpha/beta domain

pfam08785
Location:575 → 687

Ku_PK_bind; Ku C terminal domain like
NM_009533.2 → NP_033559.2 X-ray repair cross-complementing protein 5 isoform 1

See identical proteins and their annotated locations for NP_033559.2

Status: VALIDATED

Source sequence(s)

AK081633, AK165470, CJ220465

Consensus CDS

CCDS35608.1

UniProtKB/Swiss-Prot

P27641, Q3TE46, Q3TJT0, Q3TN82, Q80UT1, Q8C4N6, Q8K1K7, Q9R169

Related

ENSMUSP00000027379.9, ENSMUST00000027379.10

Conserved Domains (4) summary

cd00873
Location:244 → 542

KU80; Ku-core domain, Ku80 subfamily; Ku80 is a subunit of the Ku protein, which plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination. The mechanism underlying the ...

TIGR00578
Location:9 → 577

ku70; ATP-dependent DNA helicase II, 70 kDa subunit (ku70)

pfam03731
Location:9 → 244

Ku_N; Ku70/Ku80 N-terminal alpha/beta domain

pfam08785
Location:594 → 706

Ku_PK_bind; Ku C terminal domain like

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

72346576..72434112

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006495892.2 → XP_006495955.1 X-ray repair cross-complementing protein 5 isoform X1

Conserved Domains (3) summary

cd00873
Location:244 → 542

KU80; Ku-core domain, Ku80 subfamily; Ku80 is a subunit of the Ku protein, which plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination. The mechanism underlying the ...

pfam03731
Location:9 → 244

Ku_N; Ku70/Ku80 N-terminal alpha/beta domain

pfam08785
Location:594 → 722

Ku_PK_bind; Ku C terminal domain like
XM_006495893.4 → XP_006495956.1 X-ray repair cross-complementing protein 5 isoform X2

Conserved Domains (3) summary

cd00873
Location:225 → 523

KU80; Ku-core domain, Ku80 subfamily; Ku80 is a subunit of the Ku protein, which plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination. The mechanism underlying the ...

pfam03731
Location:1 → 225

Ku_N; Ku70/Ku80 N-terminal alpha/beta domain

pfam08785
Location:575 → 703

Ku_PK_bind; Ku C terminal domain like