Tssk1 testis-specific serine kinase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Tssk1provided by MGI
Official Full Name: testis-specific serine kinase 1provided by MGI
Primary source: MGI:MGI:1347557
See related: Ensembl:ENSMUSG00000041566 AllianceGenome:MGI:1347557
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tsk1; Tssk; TSK-1; Stk22a; Tssk1b
Summary: Enables magnesium ion binding activity and protein serine/threonine kinase activity. Involved in several processes, including peptidyl-serine phosphorylation; skin development; and spermatid development. Located in motile cilium. Is expressed in embryo; seminiferous tubule; spermatid; and testis. Orthologous to several human genes including TSSK1B (testis specific serine kinase 1B). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: human all
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Genomic context

Location:: 16 A3; 16 11.09 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	16	NC_000082.7 (17712067..17713517)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	16	NC_000082.6 (17894203..17895653)

Chromosome 16 - NC_000082.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In mouse testis, PPP1CC2 can form a complex with TSSK1 mediated by the direct interaction of each with the kinase substrate protein TSKS. Interaction between PPP1CC2 and TSKS is mediated through an RVxF docking motif on the TSKS surface.
Title: PPP1CC2 can form a kinase/phosphatase complex with the testis-specific proteins TSSK1 and TSKS in the mouse testis.
HSP90 function abolished the catalytic activities of TSSK4 and -6 but did not significantly alter the specific activities of TSSK1 and -2. Inhibition of HSP90 resulted in increased TSSK ubiquitination and proteasomal degradation
Title: Heat shock protein 90 functions to stabilize and activate the testis-specific serine/threonine kinases, a family of kinases essential for male fertility.
Tssk1 and Tssk2 play an important role in the regulation of sperm function.
Title: [Cloning and expression of Tssk1 & Tssk2 in mice and the presence & localization of them in mature sperm].
Results reveal TSSK1- and TSSK2-dependent functions of a transformed chromatoid body in post-meiotic cytodifferentiation of spermatids.
Title: Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases.
Deletion of Tssk2 kinase/substrate pair is predicted to play an indispensable role during spermiogenesis
Title: TSKS concentrates in spermatid centrioles during flagellogenesis.
Results confirm both TSSK 2 and TSKS persist in sperm, define a critical role of TSKS' N-terminus in enzyme interaction, identify Ser 281 as a TSKS phosphorylation site and indicate an indispensable role for TSSK 1 and 2 in spermiogenesis.
Title: Targeted deletion of Tssk1 and 2 causes male infertility due to haploinsufficiency.
The expression patterns of Tsk1 is documented throughout spermiogenesis showing an initial association of Tsk1 with cells in meiotic metaphase.
Title: Immunohistochemical analysis of the expression of two serine-threonine kinases in the maturing mouse testis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation

General gene information

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Markers

Stk22a (e-PCR), detects polymorphism
- UniSTS:265750

UniSTS:260493 (e-PCR)
- UniSTS:260493

Tssk1 (e-PCR), detects polymorphism
- UniSTS:260493

Stk22a (e-PCR), detects polymorphism
- UniSTS:260494

U01840 (e-PCR)
- UniSTS:125640

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatid development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in acrosomal vesicle	TAS Traceable Author Statement more info	PubMed
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cilium	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in motile cilium	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: testis-specific serine/threonine-protein kinase 1

Names: TSSK-1; serine/threonine kinase 22A (spermiogenesis associated); serine/threonine-protein kinase 22A; testis-specific kinase 1; testis-specific serine/threonine kinase

NP_033461.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009435.2 → NP_033461.2 testis-specific serine/threonine-protein kinase 1

See identical proteins and their annotated locations for NP_033461.2

Status: VALIDATED

Source sequence(s)

AK006517, BC050772, U01840

Consensus CDS

CCDS28011.1

UniProtKB/Swiss-Prot

Q61241, Q80YU1

Related

ENSMUSP00000040302.3, ENSMUST00000046937.4

Conserved Domains (2) summary

smart00220
Location:12 → 272

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14165
Location:10 → 271

STKc_TSSK1_2-like; Catalytic domain of testis-specific serine/threonine kinase 1, TSSK2, and similar proteins