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    Top3a topoisomerase (DNA) III alpha [ Mus musculus (house mouse) ]

    Gene ID: 21975, updated on 5-Mar-2024

    Summary

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    Official Symbol
    Top3aprovided by MGI
    Official Full Name
    topoisomerase (DNA) III alphaprovided by MGI
    Primary source
    MGI:MGI:1197527
    See related
    Ensembl:ENSMUSG00000002814 AllianceGenome:MGI:1197527
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Summary
    Predicted to enable DNA topoisomerase type I (single strand cut, ATP-independent) activity and single-stranded DNA binding activity. Predicted to be involved in DNA topological change; chromosome separation; and mitochondrial DNA metabolic process. Predicted to be located in PML body. Predicted to be active in nucleus. Is expressed in several structures, including adrenal gland; cardiovascular system; genitourinary system; gut; and nervous system. Human ortholog(s) of this gene implicated in autosomal recessive progressive external ophthalmoplegia with mitochondrial DNA deletions 5. Orthologous to human TOP3A (DNA topoisomerase III alpha). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in testis adult (RPKM 5.5), thymus adult (RPKM 4.9) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    11 B2; 11 37.81 cM
    Exon count:
    20
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 11 NC_000077.7 (60629014..60668099, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 11 NC_000077.6 (60738209..60777365, complement)

    Chromosome 11 - NC_000077.7Genomic Context describing neighboring genes Neighboring gene microRNA 5100 Neighboring gene mitochondrial elongation factor 2 Neighboring gene STARR-seq mESC enhancer starr_29657 Neighboring gene predicted gene, 23341 Neighboring gene STARR-seq mESC enhancer starr_29659 Neighboring gene Smith-Magenis syndrome chromosome region, candidate 8 homolog (human) Neighboring gene serine hydroxymethyltransferase 1 (soluble) Neighboring gene STARR-positive B cell enhancer ABC_E6992

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Regulation of mouse DNA topoisomerase IIIalpha gene expression by YY1 and USF transcription factors. Park EJ, et al. Biochem Biophys Res Commun, 2001 May 4. PMID 11327713
    2. Isolation of a cDNA encoding mouse DNA topoisomerase III which is highly expressed at the mRNA level in the testis. Seki T, et al. Biochim Biophys Acta, 1998 Mar 9. PMID 9540825
    3. Mammalian DNA topoisomerase IIIalpha is essential in early embryogenesis. Li W, et al. Proc Natl Acad Sci U S A, 1998 Feb 3. PMID 9448276, Free PMC Article
    4. Genome instability and embryonic developmental defects in RMI1 deficient mice. Guiraldelli MF, et al. DNA Repair (Amst), 2013 Oct. PMID 23900276, Free PMC Article
    5. Role of replication protein A in double holliday junction dissolution mediated by the BLM-Topo IIIα-RMI1-RMI2 protein complex. Xue X, et al. J Biol Chem, 2013 May 17. PMID 23543748, Free PMC Article

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (3)  1 citation
    • Endonuclease-mediated (1) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC106383

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA topoisomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables single-stranded DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA recombination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA topological change IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA topological change ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in chromosome separation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in double-strand break repair via homologous recombination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in mitochondrial DNA metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in resolution of DNA recombination intermediates ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in PML body ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of RecQ family helicase-topoisomerase III complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of RecQ family helicase-topoisomerase III complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA topoisomerase 3-alpha
    Names
    DNA topoisomerase III alpha
    Top IIIa
    topoisomerase (DNA) 3 alpha
    topoisomerase 3 alpha
    NP_033436.1
    XP_036012518.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_009410.4 → NP_033436.1  DNA topoisomerase 3-alpha

      See identical proteins and their annotated locations for NP_033436.1

      Status: VALIDATED

      Source sequence(s)
      AL596215
      Consensus CDS
      CCDS24797.1
      UniProtKB/Swiss-Prot
      O70157
      UniProtKB/TrEMBL
      Q3V3P1, Q5NCT2
      Related
      ENSMUSP00000002891.5, ENSMUST00000002891.11
      Conserved Domains (6) summary
      cd03362
      Location:35 → 193
      TOPRIM_TopoIA_TopoIII; TOPRIM_TopoIA_TopoIII: The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving ...
      COG0550
      Location:35 → 635
      TopA; DNA topoisomerase IA [Replication, recombination and repair]
      COG0551
      Location:652 → 722
      YrdD; ssDNA-binding Zn-finger and Zn-ribbon domains of topoisomerase 1 [Replication, recombination and repair]
      cd00186
      Location:202 → 617
      TOP1Ac; DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA ...
      pfam01396
      Location:655 → 694
      zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger
      pfam06839
      Location:897 → 941
      zf-GRF; GRF zinc finger

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000077.7 Reference GRCm39 C57BL/6J

      Range
      60629014..60668099 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036156625.1 → XP_036012518.1  DNA topoisomerase 3-alpha isoform X1

      UniProtKB/TrEMBL
      Q3V3P1
      Conserved Domains (4) summary
      cd03362
      Location:4 → 134
      TOPRIM_TopoIA_TopoIII; The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving one strand of the DNA ...
      cd00186
      Location:143 → 558
      TOP1Ac; DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA ...
      pfam01396
      Location:596 → 635
      zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger
      pfam06839
      Location:838 → 882
      zf-GRF; GRF zinc finger
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O70157.1 GenPept UniProtKB/Swiss-Prot:O70157

    Gene LinkOut

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