Agap3 ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Agap3provided by MGI
Official Full Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 3provided by MGI
Primary source: MGI:MGI:2183446
See related: Ensembl:ENSMUSG00000023353 AllianceGenome:MGI:2183446
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Crag; Crag.; AGAP-3; Centg3; MRIP-1; cnt-g3
Summary: Enables GTPase activity and polyubiquitin modification-dependent protein binding activity. Acts upstream of or within cellular response to reactive oxygen species; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein import into nucleus. Located in cell periphery; cytoplasm; and nucleus. Orthologous to human AGAP3 (ArfGAP with GTPase domain, ankyrin repeat and PH domain 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in cerebellum adult (RPKM 21.6), cortex adult (RPKM 20.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 A3; 5 11.81 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (24657175..24707045)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (24452177..24502047)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Novelty-induced memory consolidation is accompanied by increased Agap3 transcription: a cross-species study.
Title: Novelty-induced memory consolidation is accompanied by increased Agap3 transcription: a cross-species study.
Forebrain-specific deficiency of the GTPase CRAG/Centaurin-gamma3 leads to immature dentate gyri and hyperactivity in mice.
Title: Forebrain-specific deficiency of the GTPase CRAG/Centaurin-γ3 leads to immature dentate gyri and hyperactivity in mice.
CRAG enhances the cell survival signal against the accumulation of unfolded proteins, including polyQ, through not only proteasome activation, but also the activation of c-Fos-dependent AP-1.
Title: CRMP5-associated GTPase (CRAG) protein protects neuronal cells against cytotoxicity of expanded polyglutamine protein partially via c-Fos-dependent activator protein-1 activation.
We propose that CRAG is a modulator of promyelocytic leukemia protein function and dynamics in reactive oxygen species signaling and is protectively involved in the pathogenesis of polyglutamine diseases.
Title: A novel GTPase, CRAG, mediates promyelocytic leukemia protein-associated nuclear body formation and degradation of expanded polyglutamine protein.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH128009 (e-PCR)
- UniSTS:211318

Agap3 (e-PCR), detects polymorphism
- UniSTS:527242

AV028425 (e-PCR)
- UniSTS:164277

17.MMHAP83FRF8.seq (e-PCR)
- UniSTS:123356

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within PML body organization	ISO Inferred from Sequence Orthology more info	PubMed
involved_in activation of GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cellular response to UV	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within cellular response to reactive oxygen species	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to reactive oxygen species	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within proteasome-mediated ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein import into nucleus	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in PML body	ISO Inferred from Sequence Orthology more info	PubMed
located_in cell periphery	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3

Names: CRAM-associated GTPase; MR1-interacting protein; centaurin, gamma 3; spidin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001256431.1 → NP_001243360.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks several exons and includes an alternate 3' terminal exon, compared to variant 1. It encodes isoform b which is shorter and has a distinct C-terminus, compared to isoform a.

Source sequence(s)

AC120353

UniProtKB/TrEMBL

A0A1D5RMG4

Related

ENSMUSP00000148770.2, ENSMUST00000212381.2

Conserved Domains (2) summary

cd04103
Location:127 → 284

Centaurin_gamma; Centaurin gamma (CENTG) GTPase

smart00010
Location:125 → 290

small_GTPase; Small GTPase of the Ras superfamily; ill-defined subfamily
NM_001359881.1 → NP_001346810.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1.

Source sequence(s)

AC120353

UniProtKB/Swiss-Prot

Q812F7, Q8VHH5

Conserved Domains (5) summary

cd04103
Location:127 → 284

Centaurin_gamma; Centaurin gamma (CENTG) GTPase

cd00204
Location:685 → 774

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd01250
Location:400 → 541

PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain

sd00045
Location:685 → 715

ANK; ANK repeat [structural motif]

pfam01412
Location:560 → 674

ArfGap; Putative GTPase activating protein for Arf
NM_139153.2 → NP_631892.2 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform a

See identical proteins and their annotated locations for NP_631892.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

Source sequence(s)

AC120353, AK079516, AV241066, BM943676, BM943884, CJ052480, CJ053222

Consensus CDS

CCDS19122.1

UniProtKB/Swiss-Prot

Q812F7, Q8VHH5

UniProtKB/TrEMBL

F8VQE9

Related

ENSMUSP00000024123.5, ENSMUST00000024123.9

Conserved Domains (6) summary

cd04103
Location:127 → 284

Centaurin_gamma; Centaurin gamma (CENTG) GTPase

PRK07003
Location:462 → 573

PRK07003; DNA polymerase III subunit gamma/tau

cd01250
Location:400 → 644

PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain

sd00045
Location:788 → 818

ANK; ANK repeat [structural motif]

cd08855
Location:660 → 769

ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3

pfam12796
Location:788 → 878

Ank_2; Ankyrin repeats (3 copies)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

24657175..24707045

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006535676.5 → XP_006535739.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X4

UniProtKB/TrEMBL

A0A0G2JDW1

Related

ENSMUSP00000142529.2, ENSMUST00000199856.2

Conserved Domains (1) summary

cd04103
Location:310 → 467

Centaurin_gamma; Centaurin gamma (CENTG) GTPase
XM_006535673.5 → XP_006535736.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X1

Conserved Domains (6) summary

cd04103
Location:310 → 467

Centaurin_gamma; Centaurin gamma (CENTG) GTPase

PRK07003
Location:645 → 756

PRK07003; DNA polymerase III subunit gamma/tau

cd01250
Location:583 → 827

PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain

sd00045
Location:971 → 1001

ANK; ANK repeat [structural motif]

cd08855
Location:843 → 952

ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3

pfam12796
Location:971 → 1061

Ank_2; Ankyrin repeats (3 copies)
XM_006535674.5 → XP_006535737.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X2

Conserved Domains (5) summary

cd04103
Location:310 → 467

Centaurin_gamma; Centaurin gamma (CENTG) GTPase

cd01250
Location:583 → 781

PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain

sd00045
Location:925 → 955

ANK; ANK repeat [structural motif]

cd08855
Location:797 → 906

ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3

pfam12796
Location:925 → 1015

Ank_2; Ankyrin repeats (3 copies)
XM_006535675.5 → XP_006535738.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X3

Conserved Domains (5) summary

cd04103
Location:310 → 467

Centaurin_gamma; Centaurin gamma (CENTG) GTPase

cd01250
Location:583 → 724

PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain

sd00045
Location:868 → 898

ANK; ANK repeat [structural motif]

cd08855
Location:740 → 849

ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3

pfam12796
Location:868 → 958

Ank_2; Ankyrin repeats (3 copies)
XM_006535677.4 → XP_006535740.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X5

Conserved Domains (5) summary

PRK07003
Location:117 → 228

PRK07003; DNA polymerase III subunit gamma/tau

cd01250
Location:55 → 299

PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain

sd00045
Location:443 → 473

ANK; ANK repeat [structural motif]

cd08855
Location:315 → 424

ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3

pfam12796
Location:443 → 533

Ank_2; Ankyrin repeats (3 copies)
XM_030254355.1 → XP_030110215.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform X6

UniProtKB/TrEMBL

A0A0G2JER6

Related

ENSMUSP00000142875.2, ENSMUST00000197513.2

Conserved Domains (4) summary

cd01250
Location:55 → 196

PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain

sd00045
Location:340 → 370

ANK; ANK repeat [structural motif]

cd08855
Location:212 → 321

ArfGap_AGAP3; ArfGAP with GTPase domain, ANK repeat and PH domain 3

pfam12796
Location:340 → 430

Ank_2; Ankyrin repeats (3 copies)