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    Mylk3 myosin light chain kinase 3 [ Mus musculus (house mouse) ]

    Gene ID: 213435, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Mylk3provided by MGI
    Official Full Name
    myosin light chain kinase 3provided by MGI
    Primary source
    MGI:MGI:2443063
    See related
    Ensembl:ENSMUSG00000031698 AllianceGenome:MGI:2443063
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    MLCK; D830007F02Rik
    Summary
    Enables calmodulin-dependent protein kinase activity and myosin light chain kinase activity. Involved in protein phosphorylation and sarcomere organization. Located in cytoplasm. Colocalizes with actin cytoskeleton. Is expressed in heart atrium; heart ventricle; inner ear; and liver. Orthologous to human MYLK3 (myosin light chain kinase 3). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in heart adult (RPKM 54.9), liver E14 (RPKM 12.4) and 2 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    8 C3; 8 41.61 cM
    Exon count:
    15
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 8 NC_000074.7 (86050933..86112969, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 8 NC_000074.6 (85324928..85386340, complement)

    Chromosome 8 - NC_000074.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E9120 Neighboring gene origin recognition complex, subunit 6 Neighboring gene predicted gene, 22336 Neighboring gene RIKEN cDNA 4921524J17 gene Neighboring gene STARR-positive B cell enhancer ABC_E3790 Neighboring gene STARR-positive B cell enhancer ABC_E11392 Neighboring gene predicted gene, 53446

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Restoration of Cardiac Myosin Light Chain Kinase Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin. Hitsumoto T, et al. Circulation, 2023 Jun 20. PMID 37128901, Free PMC Article
    2. Heterozygous Mylk3 Knockout Mice Partially Recapitulate Human DCM With Heterozygous MYLK3 Mutations. Tougas CL, et al. Front Physiol, 2019. PMID 31244672, Free PMC Article
    3. Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation. Song KH, et al. Sci Rep, 2017 Sep 14. PMID 28912435, Free PMC Article
    4. Heart Failure Induced by Perinatal Ablation of Cardiac Myosin Light Chain Kinase. Islam YF, et al. Front Physiol, 2016. PMID 27833563, Free PMC Article
    5. Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle. Gittings W, et al. Muscle Nerve, 2016 Aug. PMID 26802366

    See all (49) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Restoration of Cardiac Myosin Light Chain Kinase Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin.
      Title: Restoration of Cardiac Myosin Light Chain Kinase Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin.
    2. A temporal Ca(2+) desensitization of myosin light chain kinase in phasic smooth muscles induced by CaMKKbeta/PP2A pathways.
      Title: A temporal Ca(2+) desensitization of myosin light chain kinase in phasic smooth muscles induced by CaMKKβ/PP2A pathways.
    3. Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null C57BL/6J mice do not.
      Title: Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null C57BL/6J mice do not.
    4. Pharmacological study revealed that lamellipodia formation mediated by arp2/3 and contractility regulated by myosin light chain kinase (MLCK) were responsible for the intriguing turning behavior of T cells climbing the ramp-like structures.
      Title: Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation.
    5. Acanthopanax senticosus polysaccharide pretreatment may be associated with inhibition of the NF-kappaB/MLCK pathway and concomitant amelioration of LPS-induced tight junction dysfunction of intestinal epithelium in endotoxemia
      Title: Acanthopanax senticosus polysaccharides-induced intestinal tight junction injury alleviation via inhibition of NF-κB/MLCK pathway in a mouse endotoxemia model.
    6. that MLCK is competing with centrally active ROCK for a limiting pool of monomer with which to drive lamellar filament assembly
      Title: Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments.
    7. This study demonstrated that catchlike property during dynamic performance in mouse hindlimb muscles with (wild-type) and without MLCK(-/-) ) the primary mechanism for PTP (myosin phosphorylation).
      Title: Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle.
    8. Ablation of cardiac Mylk3 suggests that Mylk3 plays a pivotal role in the transition from compensated to decompensated hypertrophy via cardiac myocyte atrophy and sarcomeric disorganization.
      Title: Acute heart failure with cardiomyocyte atrophy induced in adult mice by ablation of cardiac myosin light chain kinase.
    9. Data suggest that galectin-3 in hepatocellular carcinoma cells promotes cell migration by inducing RhoA GTPase activity, activating Mlck, up-regulating phosphorylation of MLC2 (myosin light chain 2), and inducing actin rearrangement.
      Title: Galectin 3 regulates HCC cell invasion by RhoA and MLCK activation.
    10. epithelial MLCK-activated brush border fanning by IFN-gamma promotes adherence and internalization of normally noninvasive enteric bacteria
      Title: Commensal bacterial endocytosis in epithelial cells is dependent on myosin light chain kinase-activated brush border fanning by interferon-γ.
    Submit: New GeneRIF Correction See all GeneRIFs (27)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Clone Names

    • MGC169607

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    NOT enables calmodulin-dependent protein kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables calmodulin-dependent protein kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables myosin light chain kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables myosin light chain kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables myosin light chain kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in cardiac myofibril assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in cardiac myofibril assembly ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in cellular response to interleukin-1 ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of sarcomere organization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of vascular permeability involved in acute inflammatory response ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in sarcomere organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in sarcomere organization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in sarcomere organization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in sarcomerogenesis ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    colocalizes_with actin cytoskeleton IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    colocalizes_with actin cytoskeleton IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    myosin light chain kinase 3
    Names
    cardiac MLCK
    cardiac-MyBP-C-associated Ca/CaM kinase
    cardiac-specific myosin light chain kinase
    putative myosin light chain kinase 3
    NP_001284541.1
    NP_780650.2
    XP_006530888.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001297612.1 → NP_001284541.1  myosin light chain kinase 3 isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses an alternate first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.
      Source sequence(s)
      AK146683, AK165216, BB843262
      Consensus CDS
      CCDS80910.1
      UniProtKB/TrEMBL
      D3Z630
      Related
      ENSMUSP00000113389.2, ENSMUST00000122452.8
      Conserved Domains (2) summary
      smart00220
      Location:361 → 613
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd14192
      Location:353 → 613
      STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3

    2. NM_175441.5 → NP_780650.2  myosin light chain kinase 3 isoform 1

      See identical proteins and their annotated locations for NP_780650.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AC134854
      Consensus CDS
      CCDS40422.1
      UniProtKB/Swiss-Prot
      B0LY41, Q3UIZ8, Q3V3V0, Q8BWD1
      UniProtKB/TrEMBL
      B2RQL6
      Related
      ENSMUSP00000034133.8, ENSMUST00000034133.14
      Conserved Domains (2) summary
      smart00220
      Location:494 → 746
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd14192
      Location:486 → 746
      STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000074.7 Reference GRCm39 C57BL/6J

      Range
      86050933..86112969 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006530825.4 → XP_006530888.1  myosin light chain kinase 3 isoform X1

      Conserved Domains (1) summary
      cd14192
      Location:326 → 586
      STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q3UIZ8.1 GenPept UniProtKB/Swiss-Prot:Q3UIZ8

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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