Slit2 slit guidance ligand 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Slit2provided by MGI
Official Full Name: slit guidance ligand 2provided by MGI
Primary source: MGI:MGI:1315205
See related: Ensembl:ENSMUSG00000031558 AllianceGenome:MGI:1315205
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Slil3; Drad-1; slit-2; mKIAA4141; b2b1200.1Clo; E030015M03Rik; E130320P19Rik
Summary: The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Expression: Broad expression in CNS E11.5 (RPKM 6.3), limb E14.5 (RPKM 5.2) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 B3; 5 26.05 cM

Exon count:: 39

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (48138633..48465077)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (47981291..48307735)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The chemorepellent, SLIT2, bolsters innate immunity against Staphylococcus aureus.
Title: The chemorepellent, SLIT2, bolsters innate immunity against Staphylococcus aureus.
Regulation of Axon Guidance by Slit2 and Netrin-1 Signaling in the Lacrimal Gland of Aqp5 Knockout Mice.
Title: Regulation of Axon Guidance by Slit2 and Netrin-1 Signaling in the Lacrimal Gland of Aqp5 Knockout Mice.
Coadaptation fostered by the SLIT2-ROBO1 axis facilitates liver metastasis of pancreatic ductal adenocarcinoma.
Title: Coadaptation fostered by the SLIT2-ROBO1 axis facilitates liver metastasis of pancreatic ductal adenocarcinoma.
Ductular reaction promotes intrahepatic angiogenesis through Slit2-Roundabout 1 signaling.
Title: Ductular reaction promotes intrahepatic angiogenesis through Slit2-Roundabout 1 signaling.
Slit2-Robo Signaling Promotes Glomerular Vascularization and Nephron Development.
Title: Slit2-Robo Signaling Promotes Glomerular Vascularization and Nephron Development.
The neurorepellent, Slit2, prevents macrophage lipid loading by inhibiting CD36-dependent binding and internalization of oxidized low-density lipoprotein.
Title: The neurorepellent, Slit2, prevents macrophage lipid loading by inhibiting CD36-dependent binding and internalization of oxidized low-density lipoprotein.
Slit2/Robo1 Mitigates DSS-induced Ulcerative Colitis by Activating Autophagy in Intestinal Stem Cell.
Title: Slit2/Robo1 Mitigates DSS-induced Ulcerative Colitis by Activating Autophagy in Intestinal Stem Cell.
Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.
Title: Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.
SLIT2/ROBO1-signaling inhibits macropinocytosis by opposing cortical cytoskeletal remodeling.
Title: SLIT2/ROBO1-signaling inhibits macropinocytosis by opposing cortical cytoskeletal remodeling.
Slit2 is involved in the activation of HSCs and liver fibrogenesis.
Title: Slit2 signaling contributes to cholestatic fibrosis in mice by activation of hepatic stellate cells.

Submit: New GeneRIF Correction See all GeneRIFs (78)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Chemically induced (ENU) (1)
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Slit2 (e-PCR), detects polymorphism
- UniSTS:526943

Slit2 (e-PCR), detects polymorphism
- UniSTS:507233

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTPase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables Roundabout binding	IBA Inferred from Biological aspect of Ancestor more info
enables Roundabout binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables chemorepellent activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables extracellular matrix structural constituent	RCA inferred from Reviewed Computational Analysis more info	PubMed
enables heparan sulfate proteoglycan binding	ISO Inferred from Sequence Orthology more info
enables heparin binding	IBA Inferred from Biological aspect of Ancestor more info
enables heparin binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables laminin-1 binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables proteoglycan binding	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in Roundabout signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in aortic valve morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon extension involved in axon guidance	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within axon guidance	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon guidance	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within axonogenesis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in branching morphogenesis of an epithelial tube	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in cell migration involved in sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell-cell adhesion	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to heparin	ISO Inferred from Sequence Orthology more info
involved_in chemorepulsion involved in postnatal olfactory bulb interneuron migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within chemotaxis	IEA Inferred from Electronic Annotation more info
involved_in corticospinal neuron axon guidance through spinal cord	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within dorsal/ventral axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within dorsal/ventral axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within in utero embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in induction of negative chemotaxis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within kidney development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mammary duct terminal end bud growth	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mammary gland duct morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within mammary gland duct morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within metanephros development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in motor neuron axon guidance	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in negative chemotaxis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of actin filament polymerization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of axon extension	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cell growth	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cellular response to growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of chemokine-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of endothelial cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of lamellipodium assembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of leukocyte chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of monocyte chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of mononuclear cell migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neutrophil chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of retinal ganglion cell axon guidance	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of small GTPase mediated signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smooth muscle cell chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smooth muscle cell migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vascular permeability	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within nervous system development	IEA Inferred from Electronic Annotation more info
involved_in neuron projection extension	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuron projection morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within neuron projection morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within olfactory bulb development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of synapse assembly	ISO Inferred from Sequence Orthology more info
involved_in pulmonary valve morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinal ganglion cell axon guidance	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within retinal ganglion cell axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within retinal ganglion cell axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retinal ganglion cell axon guidance	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within telencephalon cell migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ureteric bud development	ISO Inferred from Sequence Orthology more info
involved_in ventricular septum morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in axon	ISO Inferred from Sequence Orthology more info
located_in cell body	ISO Inferred from Sequence Orthology more info
located_in cell projection	ISO Inferred from Sequence Orthology more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in collagen-containing extracellular matrix	HDA more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular space	HDA more info	PubMed
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info

General protein information

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Preferred Names: slit homolog 2 protein

Names: downregulated during adipocyte differentiation-1; neurogenic extracellular slit protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001291227.2 → NP_001278156.1 slit homolog 2 protein isoform a precursor

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

Source sequence(s)

AC101004, AC113542, AC157351, AK053182, AW556809, BE944330

Consensus CDS

CCDS71586.1

UniProtKB/TrEMBL

B2RX05, G3UYX7

Related

ENSMUSP00000134263.2, ENSMUST00000174421.8

Conserved Domains (10) summary

smart00013
Location:27 → 58

LRRNT; Leucine rich repeat N-terminal domain

smart00082
Location:863 → 912

LRRCT; Leucine rich repeat C-terminal domain

smart00179
Location:1002 → 1033

EGF_CA; Calcium-binding EGF-like domain

smart00282
Location:1196 → 1329

LamG; Laminin G domain

cd00054
Location:1089 → 1125

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00116
Location:81 → 214

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00033
Location:38 → 56

LRR_RI; leucine-rich repeat [structural motif]

pfam12799
Location:328 → 368

LRR_4; Leucine Rich repeats (2 copies)

pfam13855
Location:781 → 841

LRR_8; Leucine rich repeat

cl15307
Location:434 → 464

TPKR_C2; Tyrosine-protein kinase receptor C2 Ig-like domain
NM_001291228.2 → NP_001278157.1 slit homolog 2 protein isoform b precursor

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks alternate in-frame exons in the 5' and central coding regions compared to variant 1. It encodes isoform b, which is shorter than isoform a.

Source sequence(s)

AC101004, AC113542, AC157351, AK053182, AW556809, BE944330

Consensus CDS

CCDS71588.1

UniProtKB/TrEMBL

B7ZNW9, G3UY21

Related

ENSMUSP00000133912.2, ENSMUST00000174313.8

Conserved Domains (8) summary

smart00013
Location:27 → 58

LRRNT; Leucine rich repeat N-terminal domain

smart00082
Location:855 → 904

LRRCT; Leucine rich repeat C-terminal domain

smart00282
Location:1179 → 1312

LamG; Laminin G domain

cd00054
Location:1072 → 1108

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00116
Location:81 → 214

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00033
Location:38 → 56

LRR_RI; leucine-rich repeat [structural motif]

pfam12799
Location:328 → 368

LRR_4; Leucine Rich repeats (2 copies)

pfam13855
Location:773 → 833

LRR_8; Leucine rich repeat
NM_178804.5 → NP_848919.3 slit homolog 2 protein isoform c precursor

See identical proteins and their annotated locations for NP_848919.3

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks three alternate in-frame exons compared to variant 1. The encoded isoform (c) is shorter than isoform a.

Source sequence(s)

AC101004, AK033292, AK053182, AW556809, BC079911, BE944330

Consensus CDS

CCDS19280.1

UniProtKB/Swiss-Prot

E9QKB4, Q9R1B9, Q9Z166

UniProtKB/TrEMBL

B7ZNW9

Related

ENSMUSP00000133840.3, ENSMUST00000173107.8

Conserved Domains (8) summary

smart00013
Location:27 → 58

LRRNT; Leucine rich repeat N-terminal domain

smart00082
Location:851 → 900

LRRCT; Leucine rich repeat C-terminal domain

smart00282
Location:1175 → 1308

LamG; Laminin G domain

cd00054
Location:1068 → 1104

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00116
Location:81 → 214

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00033
Location:38 → 56

LRR_RI; leucine-rich repeat [structural motif]

pfam12799
Location:324 → 364

LRR_4; Leucine Rich repeats (2 copies)

pfam13855
Location:769 → 829

LRR_8; Leucine rich repeat

RNA

NR_111900.2 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (4) lacks three internal exons and uses an alternate splice site compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC101004, AC113542, AC157351, AK053182, AW556809, BE944330

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

48138633..48465077

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006503815.4 → XP_006503878.1 slit homolog 2 protein isoform X3

UniProtKB/TrEMBL

A0A140T8T2, B7ZNW9

Related

ENSMUSP00000127615.4, ENSMUST00000170109.9

Conserved Domains (7) summary

TIGR00864
Location:836 → 1017

PCC; polycystin cation channel protein

smart00013
Location:27 → 58

LRRNT; Leucine rich repeat N-terminal domain

smart00282
Location:1187 → 1320

LamG; Laminin G domain

cd00054
Location:1080 → 1116

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

sd00033
Location:38 → 56

LRR_RI; leucine-rich repeat [structural motif]

pfam13855
Location:782 → 841

LRR_8; Leucine rich repeat

NF033189
Location:561 → 858

internalin_A; class 1 internalin InlA
XM_006503814.4 → XP_006503877.1 slit homolog 2 protein isoform X2

UniProtKB/TrEMBL

B2RX05

Conserved Domains (8) summary

TIGR00864
Location:828 → 1009

PCC; polycystin cation channel protein

smart00013
Location:27 → 58

LRRNT; Leucine rich repeat N-terminal domain

smart00282
Location:1188 → 1321

LamG; Laminin G domain

cd00054
Location:1081 → 1117

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

sd00033
Location:38 → 56

LRR_RI; leucine-rich repeat [structural motif]

pfam00008
Location:998 → 1025

EGF; EGF-like domain

pfam13855
Location:774 → 833

LRR_8; Leucine rich repeat

NF033189
Location:553 → 850

internalin_A; class 1 internalin InlA
XM_006503813.4 → XP_006503876.1 slit homolog 2 protein isoform X1

UniProtKB/TrEMBL

B2RX05

Conserved Domains (8) summary

TIGR00864
Location:832 → 1013

PCC; polycystin cation channel protein

smart00013
Location:27 → 58

LRRNT; Leucine rich repeat N-terminal domain

smart00282
Location:1192 → 1325

LamG; Laminin G domain

cd00054
Location:1085 → 1121

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

sd00033
Location:38 → 56

LRR_RI; leucine-rich repeat [structural motif]

pfam00008
Location:1002 → 1029

EGF; EGF-like domain

pfam13855
Location:778 → 837

LRR_8; Leucine rich repeat

NF033189
Location:557 → 854

internalin_A; class 1 internalin InlA
XM_006503816.4 → XP_006503879.1 slit homolog 2 protein isoform X4

UniProtKB/TrEMBL

B7ZNW9

Conserved Domains (7) summary

TIGR00864
Location:832 → 1013

PCC; polycystin cation channel protein

smart00013
Location:27 → 58

LRRNT; Leucine rich repeat N-terminal domain

smart00282
Location:1183 → 1316

LamG; Laminin G domain

cd00054
Location:1076 → 1112

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

sd00033
Location:38 → 56

LRR_RI; leucine-rich repeat [structural motif]

pfam13855
Location:778 → 837

LRR_8; Leucine rich repeat

NF033189
Location:557 → 854

internalin_A; class 1 internalin InlA