Rasgrf2 RAS protein-specific guanine nucleotide-releasing factor 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rasgrf2provided by MGI
Official Full Name: RAS protein-specific guanine nucleotide-releasing factor 2provided by MGI
Primary source: MGI:MGI:109137
See related: Ensembl:ENSMUSG00000021708 AllianceGenome:MGI:109137
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Grf2; Ras-GRF2; 6330417G04Rik
Summary: Predicted to enable guanyl-nucleotide exchange factor activity. Acts upstream of or within long-term synaptic potentiation and regulation of NMDA receptor activity. Predicted to be located in endoplasmic reticulum. Is expressed in brain; hypothalamus mantle layer; medulla oblongata basal plate mantle layer; and pons mantle layer. Orthologous to human RASGRF2 (Ras protein specific guanine nucleotide releasing factor 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in frontal lobe adult (RPKM 11.9), cortex adult (RPKM 11.9) and 14 other tissues See more
Orthologs: human all
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Genomic context

Location:: 13 C3; 13 47.43 cM

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (92028526..92268584, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (91880407..92131828, complement)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GRF2 Is Crucial for Cone Photoreceptor Viability and Ribbon Synapse Formation in the Mouse Retina.
Title: GRF2 Is Crucial for Cone Photoreceptor Viability and Ribbon Synapse Formation in the Mouse Retina.
Impaired contextual fear conditioning in RasGRF2 mutant mice is likely Ras-ERK-dependent.
Title: Impaired contextual fear conditioning in RasGRF2 mutant mice is likely Ras-ERK-dependent.
Cocaine self administration in RasGRF2 KO mice was increased relative to WT controls, whereas RasGRF1 KO and WT mice did not differ. RasGRF2 mediates cocaine self-administration via an ERK-dependent mechanism, whereas RasGRF1 has no effect on responding for cocaine.
Title: The Inhibition of RasGRF2, But Not RasGRF1, Alters Cocaine Reward in Mice.
Nuclear migration in retinal cone photoreceptors is altered in GRF2 knockout mice.
Title: RASGRF2 controls nuclear migration in postnatal retinal cone photoreceptors.
adaptations in the noradrenergic system contribute to the Rasgrf2 enhanced risk of alcoholism.
Title: Rasgrf2 controls noradrenergic involvement in the acute and subchronic effects of alcohol in the brain.
Findings indicate that GRF2 regulates both the basal level and environmentally induced increase of newborn neuron survival, as well as in the induction of a distinct form of synaptic plasticity of newborn neurons that contributes to learning and memory
Title: Ras-GRF2 mediates long-term potentiation, survival, and response to an enriched environment of newborn neurons in the hippocampus.
These data suggest that Rasgrf2 controls dopaminergic signalling and adaptations to alcohol also in other brain regions, beyond the nucleus accumbens.
Title: Rasgrf2 controls dopaminergic adaptations to alcohol in mice.
these results suggest that betaarrestin1 regulates rasgrf2 gene expression and Rac activation to affect membrane protrusion and cell migration and invasion.
Title: βArrestin1 regulates the guanine nucleotide exchange factor RasGRF2 expression and the small GTPase Rac-mediated formation of membrane protrusion and cell motility.
Ras-GRF1 and -GRF2 may act as adaptors that bind PLCgamma1 and restrict Ca2+ signalling to the vicinity of focal adhesions, indicating a new role for these GRFs that is required for IL-1 induction of the Ras-->ERK pathway and MMP-3 expression
Title: Ras-guanine-nucleotide-releasing factors 1 and 2 interact with PLCγ at focal adhesions to enable IL-1-induced Ca(2+) signalling, ERK activation and MMP-3 expression.
RASGRF2 has a role in the regulation of mesolimbic dopamine neuron activity, reward response, and alcohol use and abuse
Title: RASGRF2 regulates alcohol-induced reinforcement by influencing mesolimbic dopamine neuron activity and dopamine release.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within long-term synaptic potentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in postsynaptic modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in postsynaptic modulation of chemical synaptic transmission	IEP Inferred from Expression Pattern more info	PubMed
involved_in postsynaptic modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of NMDA receptor activity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IEP Inferred from Expression Pattern more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: ras-specific guanine nucleotide-releasing factor 2

Names: ras guanine nucleotide exchange factor 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009027.4 → NP_033053.2 ras-specific guanine nucleotide-releasing factor 2

See identical proteins and their annotated locations for NP_033053.2

Status: VALIDATED

Source sequence(s)

AC146698, AC167972, U67326

UniProtKB/Swiss-Prot

P70392, Q3TYN3, Q9QX51

Related

ENSMUSP00000116892.3, ENSMUST00000151408.9

Conserved Domains (7) summary

smart00147
Location:950 → 1186

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

smart00229
Location:852 → 924

RasGEFN; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif

smart00233
Location:480 → 588

PH; Pleckstrin homology domain

cd13261
Location:19 → 158

PH_RasGRF1_2; Ras-specific guanine nucleotide-releasing factors 1 and 2 Pleckstrin homology (PH) domain

pfam00169
Location:24 → 133

PH; PH domain

pfam00618
Location:638 → 686

RasGEF_N; RasGEF N-terminal motif

pfam00621
Location:247 → 428

RhoGEF; RhoGEF domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

92028526..92268584 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036157921.1 → XP_036013814.1 ras-specific guanine nucleotide-releasing factor 2 isoform X1

Conserved Domains (6) summary

smart00147
Location:951 → 1187

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases

smart00233
Location:480 → 588

PH; Pleckstrin homology domain

smart00325
Location:247 → 428

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd13261
Location:19 → 158

PH_RasGRF1_2; Ras-specific guanine nucleotide-releasing factors 1 and 2 Pleckstrin homology (PH) domain

pfam00618
Location:638 → 686

RasGEF_N; RasGEF N-terminal motif

cl02520
Location:853 → 925

REM; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately ...
XM_036157922.1 → XP_036013815.1 ras-specific guanine nucleotide-releasing factor 2 isoform X2

Conserved Domains (4) summary

smart00233
Location:480 → 588

PH; Pleckstrin homology domain

smart00325
Location:247 → 428

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases

cd13261
Location:19 → 158

PH_RasGRF1_2; Ras-specific guanine nucleotide-releasing factors 1 and 2 Pleckstrin homology (PH) domain

pfam00618
Location:638 → 686

RasGEF_N; RasGEF N-terminal motif