Prkg1 protein kinase, cGMP-dependent, type I [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkg1provided by MGI
Official Full Name: protein kinase, cGMP-dependent, type Iprovided by MGI
Primary source: MGI:MGI:108174
See related: Ensembl:ENSMUSG00000052920 AllianceGenome:MGI:108174
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CGKI; Prkg1b; Gm19690; Prkgr1b
Summary: Enables cGMP binding activity and cGMP-dependent protein kinase activity. Involved in protein phosphorylation and signal transduction. Acts upstream of or within cGMP-mediated signaling; nervous system development; and relaxation of vascular associated smooth muscle. Located in Golgi apparatus and plasma membrane. Is expressed in several structures, including bone; central nervous system; genitourinary system; gut; and respiratory system. Human ortholog(s) of this gene implicated in thoracic aortic aneurysm. Orthologous to human PRKG1 (protein kinase cGMP-dependent 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in testis adult (RPKM 1.7), CNS E11.5 (RPKM 1.6) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 C1; 19 25.17 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (30541887..31742675, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (30564487..31765268, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ferulic acid ameliorates lipopolysaccharide-induced tracheal injury via cGMP/PKGII signaling pathway.
Title: Ferulic acid ameliorates lipopolysaccharide-induced tracheal injury via cGMP/PKGII signaling pathway.
Protein kinase G1 regulates bone regeneration and rescues diabetic fracture healing.
Title: Protein kinase G1 regulates bone regeneration and rescues diabetic fracture healing.
The endothelial nitric oxide synthase/cyclic guanosine monophosphate/protein kinase G pathway activates primordial follicles.
Title: The endothelial nitric oxide synthase/cyclic guanosine monophosphate/protein kinase G pathway activates primordial follicles.
These results suggest that cGKI in vascular smooth muscle cells should be targeted by therapies designed to protect brain tissue against ischemic stroke.
Title: cGMP-dependent protein kinase I in vascular smooth muscle cells improves ischemic stroke outcome in mice.
Increased oxidation of PKGIalpha to its disulfide-activated state found in pulmonary arteries from mice with hypoxic pulmonary arterial hypertension (PH) or lungs from patients. It caused by chronic hypoxia, and it limits progression of PH by pulmonary vasodilation that counters and offsets maladaptive vasoconstriction, and also disulfide PKGIalpha is protective by preventing maladaptive growth and fibrosis signaling.
Title: Oxidation of PKGIα mediates an endogenous adaptation to pulmonary hypertension.
Study shows that mice carrying gain-of-function mutation in PRKG1 recapitulate the human early-onset thoracic aortic disease, and that pathological changes in the aorta are, at least in part, caused by oxidative stress, revealing that increased basal PKG activity induces oxidative stress in the aorta, raising concern about the widespread clinical use of PKG-activating drugs.
Title: Aortic pathology from protein kinase G activation is prevented by an antioxidant vitamin B(12) analog.
the NF-kappaB/miR-155-5p/PKG1 axis may be crucial in the pathogenesis of inflammatory vascular diseases, such as atherosclerotic intimal hyperplasia and preeclamptic hypertension.
Title: TNF-α elicits phenotypic and functional alterations of vascular smooth muscle cells by miR-155-5p-dependent down-regulation of cGMP-dependent kinase 1.
Prkg1 cascade role in platelet activation and thrombosis.
Title: A shear-dependent NO-cGMP-cGKI cascade in platelets acts as an auto-regulatory brake of thrombosis.
These data thus demonstrate the existence of two differently distributed cGKI isoforms in the dorsal root ganglion, and may provide insight into the cellular and molecular mechanisms of pain.
Title: Two isoforms of cyclic GMP-dependent kinase-I exhibit distinct expression patterns in the adult mouse dorsal root ganglion.
This study demonstrates that H- ras deletion protects against AngII-induced cardiac remodeling, possibly via a mechanism in which PKG-Ibeta overexpression could play a partial role, and points to H-Ras and/or downstream proteins as potential therapeutic targets in cardiovascular disease.
Title: H- ras deletion protects against angiotensin II-induced arterial hypertension and cardiac remodeling through protein kinase G-Iβ pathway activation.

Submit: New GeneRIF Correction See all GeneRIFs (100)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Transposon induced (1)
Targeted (9) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI843588 (e-PCR)
- UniSTS:166144

Cstf2t (e-PCR), detects polymorphism
- UniSTS:478995

42.MMHAP53FRF12.seq (e-PCR)
- UniSTS:124464

37.MMHAP12FRE10.seq (e-PCR)
- UniSTS:124216

RH126423 (e-PCR)
- UniSTS:163131

NoName (e-PCR)
- UniSTS:224590

NoName (e-PCR)
- UniSTS:237921

Prkg1 (e-PCR), detects polymorphism
- UniSTS:525463

RH126283 (e-PCR)
- UniSTS:162991

39.MMHAP81FRE12.seq (e-PCR)
- UniSTS:186333

AW125416 (e-PCR)
- UniSTS:181219

NoName (e-PCR)
- UniSTS:224999

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cGMP binding	IDA Inferred from Direct Assay more info	PubMed
enables cGMP-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables cGMP-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
enables calcium channel regulator activity	ISO Inferred from Sequence Orthology more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cGMP-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in collateral sprouting	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within dendrite development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within forebrain development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within metabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of platelet aggregation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of vascular associated smooth muscle cell migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vascular associated smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein kinase A signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of GTPase activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within relaxation of vascular associated smooth muscle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cGMP-dependent protein kinase 1

Names: cGK 1; cGK1; cGMP-dependent protein kinase I

NP_001013855.1

EC 2.7.11.12

NP_001398110.1

EC 2.7.11.12

NP_035290.1

EC 2.7.11.12

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001013833.3 → NP_001013855.1 cGMP-dependent protein kinase 1 isoform alpha

See identical proteins and their annotated locations for NP_001013855.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) has an alternate 5' exon, as compared to variant 2. The resulting isoform (alpha) is shorter and has a different N-terminus, as compared to isoform beta.

Source sequence(s)

AC103405, AK036955, AK052877, AK083991, CJ258300

Consensus CDS

CCDS29745.1

UniProtKB/Swiss-Prot

P0C605, Q14DK6, Q9Z0Z0

UniProtKB/TrEMBL

Q8BND1

Related

ENSMUSP00000067576.6, ENSMUST00000065067.14

Conserved Domains (4) summary

cd12085
Location:2 → 49

DD_cGKI-alpha; Dimerization/Docking domain of Cyclic GMP-dependent Protein Kinase I alpha

smart00133
Location:621 → 664

S_TK_X; Extension to Ser/Thr-type protein kinases

cd00038
Location:222 → 326

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:366 → 626

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)
NM_001411181.1 → NP_001398110.1 cGMP-dependent protein kinase 1 isoform alpha

Status: VALIDATED

Source sequence(s)

AC102751, AC103405, AC108399, AC116507, AC119158, AC122548

UniProtKB/Swiss-Prot

P0C605, Q14DK6, Q9Z0Z0

UniProtKB/TrEMBL

Q8BND1
NM_011160.3 → NP_035290.1 cGMP-dependent protein kinase 1 isoform beta

See identical proteins and their annotated locations for NP_035290.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) encodes the longer isoform (beta).

Source sequence(s)

AC103405, AF084547, AK036955, AK052877, AK083991, CJ258300

Consensus CDS

CCDS29746.1

UniProtKB/Swiss-Prot

P0C605

UniProtKB/TrEMBL

Q8BND1

Related

ENSMUSP00000073268.5, ENSMUST00000073581.6

Conserved Domains (6) summary

PTZ00263
Location:368 → 676

PTZ00263; protein kinase A catalytic subunit; Provisional

cd12086
Location:4 → 55

DD_cGKI-beta; Dimerization/Docking domain of Cyclic GMP-dependent Protein Kinase I beta

smart00133
Location:636 → 679

S_TK_X; Extension to Ser/Thr-type protein kinases

COG0664
Location:116 → 218

Crp; cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases [Signal transduction mechanisms]

cd00038
Location:237 → 341

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd05572
Location:381 → 641

STKc_cGK; Catalytic domain of the Serine/Threonine Kinase, cGMP-dependent protein kinase (cGK or PKG)