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    Prim2 DNA primase, p58 subunit [ Mus musculus (house mouse) ]

    Gene ID: 19076, updated on 11-Apr-2024

    Summary

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    Official Symbol
    Prim2provided by MGI
    Official Full Name
    DNA primase, p58 subunitprovided by MGI
    Primary source
    MGI:MGI:97758
    See related
    Ensembl:ENSMUSG00000026134 AllianceGenome:MGI:97758
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Summary
    Predicted to enable 4 iron, 4 sulfur cluster binding activity and DNA/RNA hybrid binding activity. Involved in DNA replication, synthesis of RNA primer. Part of alpha DNA polymerase:primase complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Orthologous to human PRIM2 (DNA primase subunit 2). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in liver E14 (RPKM 13.3), liver E14.5 (RPKM 11.6) and 21 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    1 12.7 cM; 1 B
    Exon count:
    21
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (33492889..33708867, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (33453808..33669808, complement)

    Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene PRP3 pre-mRNA processing factor 3 homolog pseudogene Neighboring gene predicted gene, 23453 Neighboring gene ubiquitin-40S ribosomal protein S27a pseudogene Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:33635449-33635558 Neighboring gene STARR-seq mESC enhancer starr_00539 Neighboring gene STARR-positive B cell enhancer mm9_chr1:33719891-33720191 Neighboring gene RIKEN cDNA 1700001G17 gene Neighboring gene STARR-positive B cell enhancer mm9_chr1:33726515-33726816 Neighboring gene ATPase, H+ transporting, lysosomal V1 subunit G1 pseudogene Neighboring gene predicted gene, 32229

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. E2F regulates growth-dependent transcription of genes encoding both catalytic and regulatory subunits of mouse primase. Nishikawa N, et al. Genes Cells, 2001 Jan. PMID 11168597
    2. Identification of the nuclear localization signal of mouse DNA primase: nuclear transport of p46 subunit is facilitated by interaction with p54 subunit. Mizuno T, et al. J Cell Sci, 1996 Nov. PMID 8937981
    3. Mouse primase p49 subunit molecular cloning indicates conserved and divergent regions. Prussak CE, et al. J Biol Chem, 1989 Mar 25. PMID 2925677
    4. Physical mapping of the genes for three components of the mouse DNA replication complex: polymerase alpha to the X chromosome, primase p49 subunit to chromosome 10, and primase p58 subunit to chromosome 1. Adler DA, et al. Genomics, 1991 Apr. PMID 2037291
    5. Comprehensive analysis of differential gene expression profiles on D-galactosamine-induced acute mouse liver injury and regeneration. Chung H, et al. Toxicology, 2006 Oct 3. PMID 16962228

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (1) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA/RNA hybrid binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables iron-sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA replication initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication initiation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in DNA replication, synthesis of primer IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA replication, synthesis of primer IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA replication, synthesis of primer ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of DNA primase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    part_of alpha DNA polymerase:primase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of alpha DNA polymerase:primase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of alpha DNA polymerase:primase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of alpha DNA polymerase:primase complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of primosome complex IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    DNA primase large subunit
    Names
    DNA primase 58 kDa subunit
    NP_001407370.1
    NP_001407371.1
    NP_001407372.1
    NP_001407373.1
    NP_001407374.1
    NP_001407375.1
    NP_001407376.1
    NP_001407378.1
    NP_001407379.1
    NP_001407380.1
    NP_032948.1
    XP_006495856.1
    XP_006495860.1
    XP_011236757.1
    XP_030108155.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001420441.1 → NP_001407370.1  DNA primase large subunit isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435

    2. NM_001420442.1 → NP_001407371.1  DNA primase large subunit isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435

    3. NM_001420443.1 → NP_001407372.1  DNA primase large subunit isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435

    4. NM_001420444.1 → NP_001407373.1  DNA primase large subunit isoform 5

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435

    5. NM_001420445.1 → NP_001407374.1  DNA primase large subunit isoform 6

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435

    6. NM_001420446.1 → NP_001407375.1  DNA primase large subunit isoform 7

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435

    7. NM_001420447.1 → NP_001407376.1  DNA primase large subunit isoform 8

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435

    8. NM_001420449.1 → NP_001407378.1  DNA primase large subunit isoform 9

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435

    9. NM_001420450.1 → NP_001407379.1  DNA primase large subunit isoform 10

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435

    10. NM_001420451.1 → NP_001407380.1  DNA primase large subunit isoform 11

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435

    11. NM_008922.3 → NP_032948.1  DNA primase large subunit isoform 1

      See identical proteins and their annotated locations for NP_032948.1

      Status: VALIDATED

      Source sequence(s)
      AC130712, AC134435
      Consensus CDS
      CCDS14862.1
      UniProtKB/Swiss-Prot
      P33610, Q3U4Z3
      UniProtKB/TrEMBL
      Q3THK0
      Related
      ENSMUSP00000027312.10, ENSMUST00000027312.11
      Conserved Domains (1) summary
      cd07322
      Location:28 → 451
      PriL_PriS_Eukaryotic; Eukaryotic core primase: Large subunit, PriL

    RNA

    1. NR_185176.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC134435

    2. NR_185177.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC134435

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000067.7 Reference GRCm39 C57BL/6J

      Range
      33492889..33708867 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011238455.3 → XP_011236757.1  DNA primase large subunit isoform X2

      Conserved Domains (1) summary
      cd07322
      Location:16 → 236
      PriL_PriS_Eukaryotic; Eukaryotic core primase: Large subunit, PriL

    2. XM_030252295.2 → XP_030108155.1  DNA primase large subunit isoform X3

      Conserved Domains (1) summary
      cl11970
      Location:28 → 235
      PriL; Archaeal/eukaryotic core primase: Large subunit, PriL

    3. XM_006495793.5 → XP_006495856.1  DNA primase large subunit isoform X1

      Conserved Domains (1) summary
      cl11970
      Location:28 → 234
      PriL; Archaeal/eukaryotic core primase: Large subunit, PriL

    4. XM_006495797.4 → XP_006495860.1  DNA primase large subunit isoform X4

      Conserved Domains (1) summary
      cl11970
      Location:28 → 231
      PriL; Archaeal/eukaryotic core primase: Large subunit, PriL
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P33610.1 GenPept UniProtKB/Swiss-Prot:P33610

    Gene LinkOut

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