Pld2 phospholipase D2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pld2provided by MGI
Official Full Name: phospholipase D2provided by MGI
Primary source: MGI:MGI:892877
See related: Ensembl:ENSMUSG00000020828 AllianceGenome:MGI:892877
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PLD1C
Summary: This gene is a member of the phospholipase D (PLD) superfamily. The encoded protein catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. Phosphatidic acid is an essential intracellular lipid second messenger for many signaling pathways and has been implicated in a variety of physiological processes including cytoskeletal organization and cell proliferation. A similar gene in human may also function as a guanine nucleotide exchange factor (GEF) for the small GTPase Rac2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Expression: Ubiquitous expression in lung adult (RPKM 7.1), subcutaneous fat pad adult (RPKM 6.6) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B3; 11 42.99 cM

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (70430318..70448936)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (70539492..70558110)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PLD2 deletion alleviates disruption of tight junctions in sepsis-induced ALI by regulating PA/STAT3 phosphorylation pathway.
Title: PLD2 deletion alleviates disruption of tight junctions in sepsis-induced ALI by regulating PA/STAT3 phosphorylation pathway.
PLD1 and PLD2 differentially regulate the balance of macrophage polarization in inflammation and tissue injury.
Title: PLD1 and PLD2 differentially regulate the balance of macrophage polarization in inflammation and tissue injury.
Phospholipase D1 and D2 Synergistically Regulate Thrombus Formation.
Title: Phospholipase D1 and D2 Synergistically Regulate Thrombus Formation.
Phospholipase D2 restores endothelial barrier function by promoting PTPN14-mediated VE-cadherin dephosphorylation.
Title: Phospholipase D2 restores endothelial barrier function by promoting PTPN14-mediated VE-cadherin dephosphorylation.
Simulated microgravity inhibits C2C12 myogenesis via phospholipase D2-induced Akt/FOXO1 regulation.
Title: Simulated microgravity inhibits C2C12 myogenesis via phospholipase D2-induced Akt/FOXO1 regulation.
An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation.
Title: An acute decrease in plasma membrane tension induces macropinocytosis via PLD2 activation.
Increased expression of both Pld1 and Pld2 occurs simultaneously with the appearance of vascular calcification in chronic kidney disease.
Title: Phospholipase D: A new mediator during high phosphate-induced vascular calcification associated with chronic kidney disease.
results indicate that Arf6 is involved in the regulation of PLD activity and PA synthesis required for efficient phagocytosis.
Title: Regulation of Phospholipase D by Arf6 during FcγR-Mediated Phagocytosis.
Pld2 deficiency is associated with increased leukocyte tissue infiltration after acute lung injury.
Title: Phospholipase D isoforms differentially regulate leukocyte responses to acute lung injury.
PLD2 is pivotal in the regulation of the integrity of epithelial tight junctions and occludin turn over, thereby implicating it in the pathogenesis of colitis.
Title: Intestinal Epithelial Cell-Specific Deletion of PLD2 Alleviates DSS-Induced Colitis by Regulating Occludin.

Submit: New GeneRIF Correction See all GeneRIFs (46)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (10) 1 citation
Endonuclease-mediated (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pld2 (e-PCR), detects polymorphism
- UniSTS:507159

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables N-acylphosphatidylethanolamine-specific phospholipase D activity	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol binding	IEA Inferred from Electronic Annotation more info
enables phospholipase D activity	IBA Inferred from Biological aspect of Ancestor more info
enables phospholipase D activity	IDA Inferred from Direct Assay more info	PubMed
enables phospholipase D activity	ISO Inferred from Sequence Orthology more info
enables phospholipase D activity	TAS Traceable Author Statement more info
enables protein kinase C binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within G protein-coupled receptor internalization	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in phosphatidic acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phospholipid catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipid catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mast cell degranulation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of receptor-mediated endocytosis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within receptor-mediated endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of vesicle-mediated transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to hydrogen peroxide	ISO Inferred from Sequence Orthology more info
involved_in response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in response to organic cyclic compound	ISO Inferred from Sequence Orthology more info
involved_in response to peptide hormone	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in brush border membrane	IDA Inferred from Direct Assay more info	PubMed
located_in caveola	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	TAS Traceable Author Statement more info
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane region	IBA Inferred from Biological aspect of Ancestor more info
located_in sarcolemma	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: phospholipase D2

Names: choline phosphatase 2; phosphatidylcholine-hydrolyzing phospholipase D2

NP_001289404.1

EC 3.1.4.4

NP_001289405.1

EC 3.1.4.4

NP_001348864.1

EC 3.1.4.4

NP_032902.1

EC 3.1.4.4

XP_011247096.1

EC 3.1.4.4

XP_011247097.1

EC 3.1.4.4

XP_011247098.1

EC 3.1.4.4

XP_011247099.1

EC 3.1.4.4

XP_030101520.1

EC 3.1.4.4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001302475.1 → NP_001289404.1 phospholipase D2 isoform 1

See identical proteins and their annotated locations for NP_001289404.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AK143930, AL592547, AW047839

Consensus CDS

CCDS78979.1

UniProtKB/TrEMBL

Q3UNY4, Q6NV49

Related

ENSMUSP00000104197.4, ENSMUST00000108557.10

Conserved Domains (4) summary

PLN02866
Location:67 → 929

PLN02866; phospholipase D

cd01254
Location:180 → 309

PH_PLD; Phospholipase D pleckstrin homology (PH) domain

cl02563
Location:62 → 192

PX_domain; The Phox Homology domain, a phosphoinositide binding module

cl15239
Location:614 → 806

PLDc_SF; Catalytic domain of phospholipase D superfamily proteins
NM_001302476.1 → NP_001289405.1 phospholipase D2 isoform 3

See identical proteins and their annotated locations for NP_001289405.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) uses an alternate 3' terminal exon which results in an early stop codon, compared to variant 1. The encoded isoform (3) has a shorter C-terminus compared to isoform 1.

Source sequence(s)

AK143930, AL592547

Consensus CDS

CCDS78980.1

UniProtKB/TrEMBL

Q80ZW1

Related

ENSMUSP00000104196.2, ENSMUST00000108556.2

Conserved Domains (3) summary

cd01254
Location:180 → 309

PH_PLD; Phospholipase D pleckstrin homology (PH) domain

pfam00169
Location:206 → 309

PH; PH domain

cl02563
Location:62 → 192

PX_domain; The Phox Homology domain, a phosphoinositide binding module
NM_001361935.1 → NP_001348864.1 phospholipase D2 isoform 4

Status: REVIEWED

Source sequence(s)

AK143930, AL592547, AW047839, BY772909

UniProtKB/TrEMBL

Q3UNY4

Conserved Domains (1) summary

PLN02866
Location:67 → 885

PLN02866; phospholipase D
NM_008876.3 → NP_032902.1 phospholipase D2 isoform 2

See identical proteins and their annotated locations for NP_032902.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

Source sequence(s)

AK143930, AL592547, AW047839

Consensus CDS

CCDS24953.1

UniProtKB/Swiss-Prot

P97813

UniProtKB/TrEMBL

Q3UNY4, Q5SXG5

Related

ENSMUSP00000018429.6, ENSMUST00000018429.12

Conserved Domains (1) summary

PLN02866
Location:67 → 918

PLN02866; phospholipase D