Plcd1 phospholipase C, delta 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Plcd1provided by MGI
Official Full Name: phospholipase C, delta 1provided by MGI
Primary source: MGI:MGI:97614
See related: Ensembl:ENSMUSG00000010660 AllianceGenome:MGI:97614
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables phospholipase C activity and phospholipid binding activity. Acts upstream of or within angiogenesis; labyrinthine layer blood vessel development; and regulation of cell population proliferation. Located in cytosol. Is expressed in several structures, including alimentary system; appendicular skeleton; cranium; genitourinary system; and sensory organ. Human ortholog(s) of this gene implicated in nonsyndromic congenital nail disorder 3. Orthologous to human PLCD1 (phospholipase C delta 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in bladder adult (RPKM 20.0), colon adult (RPKM 17.5) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 F3; 9 71.32 cM

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (118900595..118922570, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (119071527..119093502, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Carbon monoxide enhances calcium transients and glucose-stimulated insulin secretion from pancreatic beta-cells by activating Phospholipase C signal pathway in diabetic mice.
Title: Carbon monoxide enhances calcium transients and glucose-stimulated insulin secretion from pancreatic β-cells by activating Phospholipase C signal pathway in diabetic mice.
PLCdelta1 Plays Central Roles in the Osmotic Activation of DeltaN-TRPV1 Channels in Mouse Supraoptic Neurons and in Murine Osmoregulation.
Title: PLCδ1 Plays Central Roles in the Osmotic Activation of ΔN-TRPV1 Channels in Mouse Supraoptic Neurons and in Murine Osmoregulation.
Intracellular acidification facilitates receptor-operated TRPC4 activation through PLCdelta1 in a Ca(2+) -dependent manner.
Title: Intracellular acidification facilitates receptor-operated TRPC4 activation through PLCδ1 in a Ca(2+) -dependent manner.
PLCD1-modified mice (F0) showed progressive hair loss after birth and the genotype of CRISPR/Cas9-induced mutations in exon 2 of PLCD1 locus, suggesting the guide RNA is effective to cause mutations that lead to hair growth defect.
Title: Abnormalities of hair structure and skin histology derived from CRISPR/Cas9-based knockout of phospholipase C-delta 1 in mice.
Results show that de novo polarisation of the embryo at the 8-cell stage is directed by phospholipase C (PLC) and protein kinase C (PKC).
Title: Actomyosin polarisation through PLC-PKC triggers symmetry breaking of the mouse embryo.
PLCd1 negatively regulates lipopolysaccharide-induced production of IL-1b and Fc gamma receptor-mediated phagocytosis in macrophages.
Title: Phospholipase C δ1 in macrophages negatively regulates TLR4-induced proinflammatory cytokine production and Fcγ receptor-mediated phagocytosis.
Data show that phospholipase C delta1 (PLCD1) transfection can induce the apoptosis of CAPAN-1 and BXPC-3 pancreatic cancer cells, arrest the cell cycle in G0 /G1 phase.
Title: [Phospholipase C δ1 (PLCD1) inhibits the proliferation, invasion and migration of CAPAN-1 and BXPC-3 pancreatic cancer cells].
the Ca(2+) dependence of the interaction between PRIP-C2 and Syt1-C2A was attributed to Ca(2+) binding with Syt1-C2A, but not PRIP-C2, using a series of mutants prepared from both C2 domains.
Title: Hetero-oligomerization of C2 domains of phospholipase C-related but catalytically inactive protein and synaptotagmin-1.
Results indicate that PLCdelta1 and PLCdelta3 are required for cardiomyocyte survival and normal cardiac function.
Title: Simultaneous loss of phospholipase Cδ1 and phospholipase Cδ3 causes cardiomyocyte apoptosis and cardiomyopathy.
Expression of PLCdelta1 is increased in ALS mouse spinal cord and in neurons from ALS mice. Genetic ablation of this protein in ALS mice significantly increases survival
Title: Genetic ablation of phospholipase C delta 1 increases survival in SOD1(G93A) mice.

Submit: New GeneRIF Correction See all GeneRIFs (23)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (3) 1 citation
Endonuclease-mediated (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Plcd1 (e-PCR), detects polymorphism
- UniSTS:507153

AW212592 (e-PCR)
- UniSTS:179990

RH126779 (e-PCR)
- UniSTS:208465

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTPase activating protein binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables calcium-dependent phospholipid binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables inositol 1,4,5 trisphosphate binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol phospholipase C activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol phospholipase C activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol-4,5-bisphosphate binding	ISO Inferred from Sequence Orthology more info
enables phospholipid binding	ISO Inferred from Sequence Orthology more info
enables phosphoric diester hydrolase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within angiogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within labyrinthine layer blood vessel development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within lipid catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylinositol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylinositol-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of inositol trisphosphate biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of norepinephrine secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cytosolic calcium ion concentration	ISO Inferred from Sequence Orthology more info
involved_in release of sequestered calcium ion into cytosol	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to calcium ion	ISO Inferred from Sequence Orthology more info
involved_in response to peptide hormone	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

Names: PLC-delta 1; phosphoinositide phospholipase C-delta-1; phospholipase C delta 1 Lf

NP_001280577.1

EC 3.1.4.11

NP_062650.1

EC 3.1.4.11

XP_006512047.1

EC 3.1.4.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001293648.1 → NP_001280577.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 1

See identical proteins and their annotated locations for NP_001280577.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AC055818, AK028749, BB839358, BY383308

Consensus CDS

CCDS90679.1

UniProtKB/TrEMBL

G5DDB7

Related

ENSMUSP00000149813.2, ENSMUST00000214470.2

Conserved Domains (7) summary

cd00275
Location:654 → 781

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd08593
Location:321 → 622

PI-PLCc_delta; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-delta

PLN02952
Location:239 → 777

PLN02952; phosphoinositide phospholipase C

cd13363
Location:48 → 165

PH_PLC_delta; Phospholipase C-delta (PLC-delta) pleckstrin homology (PH) domain

pfam00169
Location:56 → 155

PH; PH domain

pfam09279
Location:239 → 321

EF-hand_like; Phosphoinositide-specific phospholipase C, efhand-like

pfam14788
Location:185 → 234

EF-hand_10; EF hand
NM_019676.3 → NP_062650.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform 2

See identical proteins and their annotated locations for NP_062650.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded protein (isoform 2) has a distinct and shorter N-terminus, compared to isoform 1.

Source sequence(s)

AK028749, BY383308

Consensus CDS

CCDS23607.1

UniProtKB/Swiss-Prot

Q8R3B1, Q9Z1B4

Related

ENSMUSP00000010804.3, ENSMUST00000010804.4

Conserved Domains (7) summary

cd00275
Location:628 → 755

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd08593
Location:295 → 596

PI-PLCc_delta; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-delta

PLN02952
Location:213 → 751

PLN02952; phosphoinositide phospholipase C

cd13363
Location:22 → 139

PH_PLC_delta; Phospholipase C-delta (PLC-delta) pleckstrin homology (PH) domain

pfam00169
Location:30 → 129

PH; PH domain

pfam09279
Location:213 → 295

EF-hand_like; Phosphoinositide-specific phospholipase C, efhand-like

pfam14788
Location:159 → 208

EF-hand_10; EF hand

RNA

NR_121571.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (3) has an alternate 5' exon and uses an alternate internal splice site, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC055818, AK028749, BY383308

Related

ENSMUST00000213464.2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

118900595..118922570 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006511984.4 → XP_006512047.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 isoform X1

Conserved Domains (4) summary

cd00275
Location:570 → 697

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd08593
Location:237 → 538

PI-PLCc_delta; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-delta

cd16217
Location:86 → 225

EFh_PI-PLCdelta1; EF-hand motif found in phosphoinositide phospholipase C delta 1 (PI-PLC-delta1)

cl17171
Location:1 → 81

PH-like; Pleckstrin homology-like domain