Pde1b phosphodiesterase 1B, Ca2+-calmodulin dependent [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pde1bprovided by MGI
Official Full Name: phosphodiesterase 1B, Ca2+-calmodulin dependentprovided by MGI
Primary source: MGI:MGI:97523
See related: Ensembl:ENSMUSG00000022489 AllianceGenome:MGI:97523
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pde1b1
Summary: Predicted to enable 3',5'-cyclic-AMP phosphodiesterase activity; calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; and calmodulin-dependent cyclic-nucleotide phosphodiesterase activity. Acts upstream of or within several processes, including response to amphetamine; serotonin metabolic process; and visual learning. Predicted to be located in cytoplasm. Predicted to be active in neuronal cell body. Is expressed in several structures, including alimentary system; brain; genitourinary system; sensory organ; and skeleton. Orthologous to human PDE1B (phosphodiesterase 1B). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in cortex adult (RPKM 29.2), frontal lobe adult (RPKM 27.9) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 F3; 15 58.99 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (103411461..103438483)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (103503034..103530056)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

New Floxed Pde1b x CreCMV global knockout (KO) mice demonstrate depression-like behavioral resistance separate from stress-related effects.
Title: Phosphodiesterase-1b deletion confers depression-like behavioral resistance separate from stress-related effects in mice.
Pde1b knock-out mice exhibited decreased tail suspension test and forced swim test immobilization; a model for depression.
Title: Phosphodiesterase-1b (Pde1b) knockout mice are resistant to forced swim and tail suspension induced immobility and show upregulation of Pde10a.
These results prove that the kinetic properties of PDE isoforms play a major role in determining intracellular cAMP signals in response to physiological elevation of [Ca2+]i.
Title: Kinetic properties of Ca2+/calmodulin-dependent phosphodiesterase isoforms dictate intracellular cAMP dynamics in response to elevation of cytosolic Ca2+.
The present studies demonstrate increased striatal dopamine turnover in PDE1B knockout mice associated with increased baseline motor activity and an exaggerated locomotor response to dopaminergic stimulants such as methamphetamine and amphetamine.
Title: Behavioral and neurochemical characterization of mice deficient in the phosphodiesterase-1B (PDE1B) enzyme.
Data suggest a role for PDE1B in locomotor responses to psychostimulants through modulation of DARPP32-dependent pathways; however, this modulation does not necessarily impact other behaviors, such as anxiety or learning.
Title: Phosphodiesterase 1B differentially modulates the effects of methamphetamine on locomotor activity and spatial learning through DARPP32-dependent pathways: evidence from PDE1B-DARPP32 double-knockout mice.
knock-out mice exhibit locomotor hyperactivity in response to dopamine agonists and display impaired spatial learning
Title: Phosphodiesterase 1B knock-out mice exhibit exaggerated locomotor hyperactivity and DARPP-32 phosphorylation in response to dopamine agonists and display impaired spatial learning.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

L01695 (e-PCR)
- UniSTS:160512

Pde1b (e-PCR), detects polymorphism
- UniSTS:546584

RH127463 (e-PCR)
- UniSTS:210773

Pde1b (e-PCR), detects polymorphism
- UniSTS:495995

D15Mit35 (e-PCR), detects polymorphism
- UniSTS:116442

Pde1b (e-PCR), detects polymorphism
- UniSTS:507119

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 3',5'-cyclic-AMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables 3',5'-cyclic-GMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info
enables 3',5'-cyclic-nucleotide phosphodiesterase activity	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables calmodulin-activated 3',5'-cyclic-GMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin-activated 3',5'-cyclic-GMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables calmodulin-activated dual specificity 3',5'-cyclic-GMP, 3',5'-cyclic-AMP phosphodiesterase activity	IBA Inferred from Biological aspect of Ancestor more info
enables calmodulin-activated dual specificity 3',5'-cyclic-GMP, 3',5'-cyclic-AMP phosphodiesterase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables cyclic-nucleotide phosphodiesterase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphoric diester hydrolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cAMP-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to granulocyte macrophage colony-stimulating factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to macrophage colony-stimulating factor stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_positive_effect dopamine catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to amphetamine	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within response to amphetamine	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within serotonin metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within visual learning	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within visual learning	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in neuronal cell body	IBA Inferred from Biological aspect of Ancestor more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Names: 63 kDa Cam-PDE; calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B; cam-PDE 1B; phosphodiesterase 1B, Ca2+-calmodulin dependent, 63 kDa; phosphodiesterase 1B1, Ca2+-calmodulin dependent, 63 kDa

NP_001272819.1

EC 3.1.4.17

NP_001344909.1

EC 3.1.4.17

NP_032826.1

EC 3.1.4.17

XP_030104267.1

EC 3.1.4.17

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001285890.1 → NP_001272819.1 dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and contains an alternate 5' terminal exon compared to variant 1. The encoded protein (isoform 2) has a distinct N-terminus and is shorter than isoform 1.

Source sequence(s)

AC131721, AK004772, CV784321, DN173601

Consensus CDS

CCDS88861.1

UniProtKB/TrEMBL

A0A2I3BPC1, Q6PDS5

Related

ENSMUSP00000153722.2, ENSMUST00000227955.2

Conserved Domains (2) summary

pfam00233
Location:202 → 429

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

pfam08499
Location:61 → 117

PDEase_I_N; 3'5'-cyclic nucleotide phosphodiesterase N-terminal
NM_001357980.1 → NP_001344909.1 dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and contains an alternate 5' terminal exon compared to variant 1. The encoded protein (isoform 3) has a distinct N-terminus and is shorter than isoform 1.

Source sequence(s)

AC131721

UniProtKB/TrEMBL

Q6PDS5

Conserved Domains (2) summary

pfam00233
Location:202 → 427

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

pfam08499
Location:61 → 117

PDEase_I_N; 3'5'-cyclic nucleotide phosphodiesterase N-terminal
NM_008800.2 → NP_032826.1 dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform 1

See identical proteins and their annotated locations for NP_032826.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC131721, BC058531, DN173601

Consensus CDS

CCDS27904.1

UniProtKB/Swiss-Prot

O35384, Q01065

UniProtKB/TrEMBL

Q6PDS5

Related

ENSMUSP00000023132.4, ENSMUST00000023132.5

Conserved Domains (2) summary

pfam00233
Location:221 → 448

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

pfam08499
Location:80 → 136

PDEase_I_N; 3'5'-cyclic nucleotide phosphodiesterase N-terminal

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

103411461..103438483

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030248407.1 → XP_030104267.1 dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B isoform X1

UniProtKB/TrEMBL

Q6PDS5, Q9DBS6

Conserved Domains (2) summary

pfam00233
Location:181 → 408

PDEase_I; 3'5'-cyclic nucleotide phosphodiesterase

pfam08499
Location:40 → 96

PDEase_I_N; 3'5'-cyclic nucleotide phosphodiesterase N-terminal