Pak1 p21 (RAC1) activated kinase 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pak1provided by MGI
Official Full Name: p21 (RAC1) activated kinase 1provided by MGI
Primary source: MGI:MGI:1339975
See related: Ensembl:ENSMUSG00000030774 AllianceGenome:MGI:1339975
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Paka; PAK-1
Summary: Enables protein serine/threonine kinase activity. Involved in trans-synaptic signaling by endocannabinoid, modulating synaptic transmission. Acts upstream of or within several processes, including nervous system development; neurotransmitter secretion; and observational learning. Located in several cellular components, including glutamatergic synapse; growth cone; and postsynaptic density. Is active in GABA-ergic synapse. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in breast cancer; renal cell carcinoma; and schizophrenia. Orthologous to human PAK1 (p21 (RAC1) activated kinase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in cortex adult (RPKM 87.8), cerebellum adult (RPKM 86.8) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 E1; 7 53.57 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (97437748..97561588)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (97788541..97912381)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Generating a Murine PTEN Null Cell Line to Discover the Key Role of p110beta-PAK1 in Castration-Resistant Prostate Cancer Invasion.
Title: Generating a Murine PTEN Null Cell Line to Discover the Key Role of p110β-PAK1 in Castration-Resistant Prostate Cancer Invasion.
Inhibition of PAK1 generates an ameliorative effect on MPLW515L mouse model of myeloproliferative neoplasms by regulating the differentiation and survival of megakaryocytes.
Title: Inhibition of PAK1 generates an ameliorative effect on MPLW515L mouse model of myeloproliferative neoplasms by regulating the differentiation and survival of megakaryocytes.
Cardiac deficiency of P21-activated kinase 1 promotes atrial arrhythmogenesis in mice following adrenergic challenge.
Title: Cardiac deficiency of P21-activated kinase 1 promotes atrial arrhythmogenesis in mice following adrenergic challenge.
The role of P21-activated kinase (Pak1) in sinus node function.
Title: The role of P21-activated kinase (Pak1) in sinus node function.
Stimulation of Neurite Outgrowth in Cerebrocortical Neurons by Sodium Channel Activator Brevetoxin-2 Requires Both N-Methyl-D-aspartate Receptor 2B (GluN2B) and p21 Protein (Cdc42/Rac)-Activated Kinase 1 (PAK1).
Title: Stimulation of Neurite Outgrowth in Cerebrocortical Neurons by Sodium Channel Activator Brevetoxin-2 Requires Both N-Methyl-D-aspartate Receptor 2B (GluN2B) and p21 Protein (Cdc42/Rac)-Activated Kinase 1 (PAK1).
Functional Polymorphism in Pak1-3' Untranslated Region Alters Skin Tumor Susceptibility by Alternative Polyadenylation.
Title: Functional Polymorphism in Pak1-3' Untranslated Region Alters Skin Tumor Susceptibility by Alternative Polyadenylation.
PAK1 inhibition reduces tumor size and extends the lifespan of mice in a genetically engineered mouse model of Neurofibromatosis Type 2 (NF2).
Title: PAK1 inhibition reduces tumor size and extends the lifespan of mice in a genetically engineered mouse model of Neurofibromatosis Type 2 (NF2).
PAK1 Silencing Attenuated Proinflammatory Macrophage Activation and Foam Cell Formation by Increasing PPARgamma Expression.
Title: PAK1 Silencing Attenuated Proinflammatory Macrophage Activation and Foam Cell Formation by Increasing PPARγ Expression.
Disruption of the autism-related gene Pak1 causes stereocilia disorganization, hair cell loss, and deafness in mice.
Title: Disruption of the autism-related gene Pak1 causes stereocilia disorganization, hair cell loss, and deafness in mice.
A Novel PAK1-Notch1 Axis Regulates Crypt Homeostasis in Intestinal Inflammation.
Title: A Novel PAK1-Notch1 Axis Regulates Crypt Homeostasis in Intestinal Inflammation.

Submit: New GeneRIF Correction See all GeneRIFs (115)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (6) 1 citation
Endonuclease-mediated (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:494152

PMC140666P2 (e-PCR)
- UniSTS:270955

Pak1 (e-PCR), detects polymorphism
- UniSTS:498799

Pak1 (e-PCR), detects polymorphism
- UniSTS:527474

D7Mit321 (e-PCR), detects polymorphism
- UniSTS:131223

D7Dcr41 (e-PCR), detects polymorphism
- UniSTS:507800

AW045634 (e-PCR)
- UniSTS:179609

AI072234 (e-PCR)
- UniSTS:244921

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables catalytic activity	IEA Inferred from Electronic Annotation more info
enables collagen binding	ISO Inferred from Sequence Orthology more info
enables gamma-tubulin binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	TAS Traceable Author Statement more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
contributes_to small GTPase binding	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info	PubMed
involved_in actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within amygdala development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in branching morphogenesis of an epithelial tube	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell migration	ISO Inferred from Sequence Orthology more info	PubMed
involved_in cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to organic cyclic compound	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin remodeling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within dendrite development	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within dendritic spine development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of cell polarity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within exocytosis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within gamma-aminobutyric acid secretion, neurotransmission	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within glutamate secretion, neurotransmission	IGI Inferred from Genetic Interaction more info	PubMed
involved_in hepatocyte growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in localization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within metabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell growth involved in cardiac muscle cell development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell proliferation involved in contact inhibition	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuromuscular junction development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron projection morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within observational learning	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of JUN kinase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of axon extension	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of fibroblast migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intracellular estrogen receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of microtubule nucleation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of microtubule polymerization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein targeting to membrane	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of stress fiber assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular associated smooth muscle cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular associated smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein localization to cytoplasmic stress granule	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within receptor clustering	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of MAPK cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of axonogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of long-term synaptic potentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of trans-synaptic signaling by endocannabinoid, modulating synaptic transmission	EXP Inferred from Experiment more info	PubMed
involved_in regulation of trans-synaptic signaling by endocannabinoid, modulating synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of trans-synaptic signaling by endocannabinoid, modulating synaptic transmission	IEP Inferred from Expression Pattern more info	PubMed
involved_in regulation of trans-synaptic signaling by endocannabinoid, modulating synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in response to organic substance	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within transmission of nerve impulse	IGI Inferred from Genetic Interaction more info	PubMed
involved_in wound healing	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in GABA-ergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in GABA-ergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in GABA-ergic synapse	IEP Inferred from Expression Pattern more info	PubMed
is_active_in GABA-ergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in Z disc	ISO Inferred from Sequence Orthology more info
located_in actin filament	ISO Inferred from Sequence Orthology more info
located_in anchoring junction	IEA Inferred from Electronic Annotation more info
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in axon	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	TAS Traceable Author Statement more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IEP Inferred from Expression Pattern more info	PubMed
located_in growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in intercalated disc	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear membrane	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density	IEP Inferred from Expression Pattern more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
located_in ruffle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine/threonine-protein kinase PAK 1

Names: CDC42/RAC effector kinase PAK-A; P21 CDKN1A-activated kinase 1; activated protein kinase alpha; alpha-PAK; p21 protein (Cdc42/Rac)-activated kinase 1; p21-activated kinase 1; p65-PAK

NP_001344291.1

EC 2.7.11.1

NP_001344292.1

EC 2.7.11.1

NP_001344293.1

EC 2.7.11.1

NP_001344294.1

EC 2.7.11.1

NP_001390408.1

EC 2.7.11.1

NP_035165.2

EC 2.7.11.1

XP_006507499.1

EC 2.7.11.1

XP_006507500.1

EC 2.7.11.1

XP_036008662.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001357362.2 → NP_001344291.1 serine/threonine-protein kinase PAK 1 isoform 2

Status: VALIDATED

Description

Transcript Variant: Variants 2 and 6 encode the same isoform (2).

Source sequence(s)

AC120438

UniProtKB/Swiss-Prot

O88643

Conserved Domains (2) summary

cd06654
Location:256 → 551

STKc_PAK1; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 1

pfam00786
Location:74 → 128

PBD; P21-Rho-binding domain
NM_001357363.2 → NP_001344292.1 serine/threonine-protein kinase PAK 1 isoform 1

Status: VALIDATED

Description

Transcript Variant: Variants 1, 3, 4, and 5 encode the same isoform (1).

Source sequence(s)

AC120438

Consensus CDS

CCDS21464.1

UniProtKB/Swiss-Prot

O88643

UniProtKB/TrEMBL

G5E884

Conserved Domains (2) summary

cd06654
Location:248 → 543

STKc_PAK1; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 1

pfam00786
Location:74 → 132

PBD; P21-Rho-binding domain
NM_001357364.2 → NP_001344293.1 serine/threonine-protein kinase PAK 1 isoform 1

Status: VALIDATED

Description

Transcript Variant: Variants 1, 3, 4, and 5 encode the same isoform (1).

Source sequence(s)

AC120438, AC151477

Consensus CDS

CCDS21464.1

UniProtKB/Swiss-Prot

O88643

UniProtKB/TrEMBL

G5E884

Conserved Domains (2) summary

cd06654
Location:248 → 543

STKc_PAK1; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 1

pfam00786
Location:74 → 132

PBD; P21-Rho-binding domain
NM_001357365.2 → NP_001344294.1 serine/threonine-protein kinase PAK 1 isoform 1

Status: VALIDATED

Description

Transcript Variant: Variants 1, 3, 4, and 5 encode the same isoform (1).

Source sequence(s)

AC120438, AC151477

Consensus CDS

CCDS21464.1

UniProtKB/Swiss-Prot

O88643

UniProtKB/TrEMBL

G5E884

Related

ENSMUSP00000146055.2, ENSMUST00000206984.2

Conserved Domains (2) summary

cd06654
Location:248 → 543

STKc_PAK1; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 1

pfam00786
Location:74 → 132

PBD; P21-Rho-binding domain
NM_001403479.1 → NP_001390408.1 serine/threonine-protein kinase PAK 1 isoform 2

Status: VALIDATED

Description

Transcript Variant: Variants 2 and 6 encode the same isoform (2).

Source sequence(s)

AC120438
NM_011035.3 → NP_035165.2 serine/threonine-protein kinase PAK 1 isoform 1

See identical proteins and their annotated locations for NP_035165.2

Status: VALIDATED

Description

Transcript Variant: Variants 1, 3, 4, and 5 encode the same isoform (1).

Source sequence(s)

AC120438

Consensus CDS

CCDS21464.1

UniProtKB/Swiss-Prot

O88643

UniProtKB/TrEMBL

G5E884

Related

ENSMUSP00000033040.6, ENSMUST00000033040.12

Conserved Domains (2) summary

cd06654
Location:248 → 543

STKc_PAK1; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 1

pfam00786
Location:74 → 132

PBD; P21-Rho-binding domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

97437748..97561588

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006507436.5 → XP_006507499.1 serine/threonine-protein kinase PAK 1 isoform X1

See identical proteins and their annotated locations for XP_006507499.1

UniProtKB/Swiss-Prot

O88643

Conserved Domains (2) summary

cd06654
Location:256 → 551

STKc_PAK1; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 1

pfam00786
Location:74 → 128

PBD; P21-Rho-binding domain
XM_036152769.1 → XP_036008662.1 serine/threonine-protein kinase PAK 1 isoform X2

UniProtKB/Swiss-Prot

O88643

UniProtKB/TrEMBL

G5E884

Conserved Domains (2) summary

cd06654
Location:248 → 543

STKc_PAK1; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 1

pfam00786
Location:74 → 132

PBD; P21-Rho-binding domain
XM_006507437.4 → XP_006507500.1 serine/threonine-protein kinase PAK 1 isoform X1

See identical proteins and their annotated locations for XP_006507500.1

UniProtKB/Swiss-Prot

O88643

Conserved Domains (2) summary

cd06654
Location:256 → 551

STKc_PAK1; Catalytic domain of the Serine/Threonine Kinase, p21-activated kinase 1

pfam00786
Location:74 → 128

PBD; P21-Rho-binding domain