Ppp1r15a protein phosphatase 1, regulatory subunit 15A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ppp1r15aprovided by MGI
Official Full Name: protein phosphatase 1, regulatory subunit 15Aprovided by MGI
Primary source: MGI:MGI:1927072
See related: Ensembl:ENSMUSG00000040435 AllianceGenome:MGI:1927072
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Gadd34; Myd116; 9630030H21
Summary: Enables protein phosphatase binding activity and protein phosphatase regulator activity. Involved in several processes, including peptidyl-serine dephosphorylation; regulation of gene expression; and regulation of protein modification process. Acts upstream of or within endoplasmic reticulum unfolded protein response. Predicted to be located in several cellular components, including Golgi apparatus; cytosol; and mitochondrion. Predicted to be part of protein phosphatase type 1 complex. Predicted to be active in endoplasmic reticulum. Is expressed in several structures, including central nervous system; genitourinary system; gut; hemolymphoid system gland; and liver. Used to study thalassemia. Orthologous to human PPP1R15A (protein phosphatase 1 regulatory subunit 15A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in liver E14 (RPKM 79.4), liver E14.5 (RPKM 76.2) and 26 other tissues See more
Orthologs: human all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 7 B3; 7 29.34 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (45172341..45175692, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (45522917..45526268, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GADD34 suppresses eIF2alpha phosphorylation and improves cognitive function in Alzheimer's disease-model mice.
Title: GADD34 suppresses eIF2α phosphorylation and improves cognitive function in Alzheimer's disease-model mice.
GADD34 Ablation Exacerbates Retinal Degeneration in P23H RHO Mice.
Title: GADD34 Ablation Exacerbates Retinal Degeneration in P23H RHO Mice.
All-trans retinoic acid changes muscle fiber type via increasing GADD34 dependent on MAPK signal.
Title: All-trans retinoic acid changes muscle fiber type via increasing GADD34 dependent on MAPK signal.
Sensitization of the UPR by loss of PPP1R15A promotes fibrosis and senescence in IPF.
Title: Sensitization of the UPR by loss of PPP1R15A promotes fibrosis and senescence in IPF.
Knockdown of GADD34 in neonatal mutant SOD1 mice ameliorates ALS.
Title: Knockdown of GADD34 in neonatal mutant SOD1 mice ameliorates ALS.
A2aR effectively downregulated p-eIF2alpha/eIF2alpha, XBP-1, XBP-1s, BIP, and CHOP levels, whereas GADD34 was upregulated.
Title: A2a adenosine receptor agonist improves endoplasmic reticulum stress in MIN6 cell line through protein kinase A/ protein kinase B/ Cyclic adenosine monophosphate response element-binding protein/ and Growth Arrest And DNA-Damage-Inducible 34/ eukaryotic Initiation Factor 2α pathways.
Depletion of HES1 increased cell death in response to endoplasmic reticulum stress in mouse and human cells, in a manner that depended on the pro-apoptotic gene growth arrest and DNA damage-inducible protein GADD34.
Title: Hairy and enhancer of split 1 (HES1) protects cells from endoplasmic reticulum stress-induced apoptosis through repression of GADD34.
Findings highlight that the phosphatase regulator, GADD34, also functions as a kinase scaffold in response to chronic oxidative stress and recruits CK1 and oxidized TDP-43 to facilitate its phosphorylation, as seen in TDP-43 proteinopathies.
Title: Chronic oxidative stress promotes GADD34-mediated phosphorylation of the TAR DNA-binding protein TDP-43, a modification linked to neurodegeneration.
established Neuro2a cells with edited GADD34 and ATF4/GADD34 genes and found that ATF4 acts as a proapoptotic factor, but GADD34 depletion did not attenuate the expression of cleaved caspase-3 induced by tunicamycin treatment.
Title: Functional validation of ATF4 and GADD34 in Neuro2a cells by CRISPR/Cas9-mediated genome editing.
In response to endoplasmic reticulum stress, activation of PERK coordinates the integrated stress response by phosphorylating eIF2alpha, which is then quickly dephosphorylated by the GADD34 complex. Data imply dual role of the ISR in promoting and inhibiting medulloblastoma tumorigenesis.
Title: Dual role of the integrated stress response in medulloblastoma tumorigenesis.

Submit: New GeneRIF Correction See all GeneRIFs (42)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (5) 1 citation

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein phosphatase 1 binding	ISO Inferred from Sequence Orthology more info
enables protein phosphatase binding	IDA Inferred from Direct Assay more info	PubMed
enables protein phosphatase regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein phosphatase regulator activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within endoplasmic reticulum unfolded protein response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of peptidyl-threonine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of phosphoprotein phosphatase activity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein processing	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endoplasmic reticulum stress-induced eIF2 alpha dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of peptidyl-serine dephosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of peptidyl-serine dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of phosphoprotein phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of phosphoprotein phosphatase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization to endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
involved_in regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in regulation of translational initiation by eIF2 alpha dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in response to endoplasmic reticulum stress	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to endoplasmic reticulum stress	IDA Inferred from Direct Assay more info	PubMed
involved_in response to endoplasmic reticulum stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	TAS Traceable Author Statement more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
part_of protein phosphatase type 1 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of protein phosphatase type 1 complex	ISO Inferred from Sequence Orthology more info

General protein information

Go to the top of the page Help

Preferred Names: protein phosphatase 1 regulatory subunit 15A

Names: growth arrest and DNA damage-inducible protein GADD34; growth arrest and DNA-damage-inducible 34; myeloid differentiation primary response gene 116; myeloid differentiation primary response protein MyD116; protein phosphatase 1, regulatory (inhibitor) subunit 15A

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.