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    Mgat3 mannoside acetylglucosaminyltransferase 3 [ Mus musculus (house mouse) ]

    Gene ID: 17309, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Mgat3provided by MGI
    Official Full Name
    mannoside acetylglucosaminyltransferase 3provided by MGI
    Primary source
    MGI:MGI:104532
    See related
    Ensembl:ENSMUSG00000042428 AllianceGenome:MGI:104532
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    GnT-III; 1110038J12Rik
    Summary
    Enables beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity. Acts upstream of or within several processes, including amyloid-beta metabolic process; negative regulation of lysosomal protein catabolic process; and positive regulation of protein localization to early endosome. Is expressed in several structures, including alimentary system; metanephros; and nervous system. Orthologous to human MGAT3 (beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in colon adult (RPKM 15.5), kidney adult (RPKM 14.4) and 22 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    15 E1; 15 37.85 cM
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 15 NC_000081.7 (80057852..80099720)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 15 NC_000081.6 (80173651..80215519)

    Chromosome 15 - NC_000081.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 31134 Neighboring gene synaptogyrin 1 Neighboring gene STARR-seq mESC enhancer starr_39220 Neighboring gene STARR-seq mESC enhancer starr_39222 Neighboring gene STARR-positive B cell enhancer ABC_E10868 Neighboring gene predicted gene, 31253 Neighboring gene STARR-seq mESC enhancer starr_39224 Neighboring gene TGF-beta activated kinase 1/MAP3K7 binding protein 1 Neighboring gene STARR-seq mESC enhancer starr_39229 Neighboring gene STARR-seq mESC enhancer starr_39231 Neighboring gene predicted gene, 46521 Neighboring gene ribosomal protein S2 pseudogene Neighboring gene mitochondrial elongation factor 1

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Bisected, complex N-glycans and galectins in mouse mammary tumor progression and human breast cancer. Miwa HE, et al. Glycobiology, 2013 Dec. PMID 24037315, Free PMC Article
    2. Loss and recovery of Mgat3 and GnT-III Mediated E-cadherin N-glycosylation is a mechanism involved in epithelial-mesenchymal-epithelial transitions. Pinho SS, et al. PLoS One, 2012. PMID 22427986, Free PMC Article
    3. Wnt/beta-catenin signaling down-regulates N-acetylglucosaminyltransferase III expression: the implications of two mutually exclusive pathways for regulation. Xu Q, et al. J Biol Chem, 2011 Feb 11. PMID 21115490, Free PMC Article
    4. The bisecting GlcNAc on N-glycans inhibits growth factor signaling and retards mammary tumor progression. Song Y, et al. Cancer Res, 2010 Apr 15. PMID 20395209, Free PMC Article
    5. Cell-cell interaction-dependent regulation of N-acetylglucosaminyltransferase III and the bisected N-glycans in GE11 epithelial cells. Involvement of E-cadherin-mediated cell adhesion. Iijima J, et al. J Biol Chem, 2006 May 12. PMID 16537539

    See all (39) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. MiR-23a transcriptional activated by Runx2 increases metastatic potential of mouse hepatoma cell via directly targeting Mgat3
      Title: MiR-23a transcriptional activated by Runx2 increases metastatic potential of mouse hepatoma cell via directly targeting Mgat3.
    2. The MGAT3 and bisected complex N-glycans retard mouse mammary tumor progression; identification of key galectins that promote mammary tumor progression in mice is not straightforward because the galectin genes are expressed; and high levels of MGAT3.
      Title: Bisected, complex N-glycans and galectins in mouse mammary tumor progression and human breast cancer.
    3. data suggest that Fut8(-/-) mice adapted to oxidative stress, both ex vivo and in vivo, by inducing various genes including GnT-III, which may compensate for the loss of core fucose functions
      Title: The absence of core fucose up-regulates GnT-III and Wnt target genes: a possible mechanism for an adaptive response in terms of glycan function.
    4. Mgat3 glycogene expression and GnT-III-mediated glycosylation, specifically on E-cadherin, is a novel and major component of the Epithelial-Mesenchymal-Transition and the reverted process, Mesenchymal-Epithelial-Transition mechanism.
      Title: Loss and recovery of Mgat3 and GnT-III Mediated E-cadherin N-glycosylation is a mechanism involved in epithelial-mesenchymal-epithelial transitions.
    5. Roles of N-acetylglucosaminyltransferase III in epithelial-to-mesenchymal transition induced by transforming growth factor beta1 (TGF-beta1) in epithelial cell lines
      Title: Roles of N-acetylglucosaminyltransferase III in epithelial-to-mesenchymal transition induced by transforming growth factor β1 (TGF-β1) in epithelial cell lines.
    6. GnT-III expression may be precisely regulated by the interplay of E-cadherin-catenin complex-mediated cell-cell adhesion and Wnt/beta-catenin signaling
      Title: Wnt/beta-catenin signaling down-regulates N-acetylglucosaminyltransferase III expression: the implications of two mutually exclusive pathways for regulation.
    7. upregulation of GnT-III in AD brains may represent an adaptive response to protect them from additional beta-amyloid production
      Title: Protective effect of N-glycan bisecting GlcNAc residues on beta-amyloid production in Alzheimer's disease.
    8. beta1,4-N-acetylglucosaminyltransferase III inhibits the function of alpha5beta1 integrin
      Title: Introduction of bisecting GlcNAc into integrin alpha5beta1 reduces ligand binding and down-regulates cell adhesion and cell migration.
    9. mice homozygous for the Mgat3T37 mutation that express truncated GlcNAc-TIII from two mutant alleles suffer neurological and other consequences
      Title: Truncated, inactive N-acetylglucosaminyltransferase III (GlcNAc-TIII) induces neurological and other traits absent in mice that lack GlcNAc-TIII.
    10. An E-cadherin-dependent pathway plays a critical role in regulation of GnT-III expression; changes in GnT-III expression presumably contribute to intracellular signaling transduction during tissue development and homeostasis.
      Title: Cell-cell interaction-dependent regulation of N-acetylglucosaminyltransferase III and the bisected N-glycans in GE11 epithelial cells. Involvement of E-cadherin-mediated cell adhesion.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (2)  1 citation
    • Endonuclease-mediated (1) 

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables glycosyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables glycosyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in N-acetylglucosamine metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within N-acetylglucosamine metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within amyloid-beta metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within cellular response to oxidative stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within cognition IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of lysosomal protein catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of lysosomal protein catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of protein localization to early endosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein N-linked glycosylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein localization ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of protein localization to early endosome IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of cell migration ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
    Names
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
    glcNAc-T III
    NP_034925.2
    XP_006520616.1
    XP_006520617.1
    XP_006520618.1
    XP_006520619.1
    XP_006520620.1
    XP_011243787.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_010795.4 → NP_034925.2  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

      See identical proteins and their annotated locations for NP_034925.2

      Status: VALIDATED

      Source sequence(s)
      BC053040, CJ174488
      Consensus CDS
      CCDS27660.1
      UniProtKB/Swiss-Prot
      P70386, Q10470
      UniProtKB/TrEMBL
      Q5RKT9
      Related
      ENSMUSP00000043077.7, ENSMUST00000044970.7
      Conserved Domains (1) summary
      pfam04724
      Location:205 → 502
      Glyco_transf_17; Glycosyltransferase family 17

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000081.7 Reference GRCm39 C57BL/6J

      Range
      80057852..80099720
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011245485.2 → XP_011243787.1  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X2

      See identical proteins and their annotated locations for XP_011243787.1

      UniProtKB/Swiss-Prot
      P70386, Q10470
      UniProtKB/TrEMBL
      Q5RKT9
      Conserved Domains (1) summary
      pfam04724
      Location:205 → 502
      Glyco_transf_17; Glycosyltransferase family 17

    2. XM_006520556.5 → XP_006520619.1  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X2

      See identical proteins and their annotated locations for XP_006520619.1

      UniProtKB/Swiss-Prot
      P70386, Q10470
      UniProtKB/TrEMBL
      Q5RKT9
      Conserved Domains (1) summary
      pfam04724
      Location:205 → 502
      Glyco_transf_17; Glycosyltransferase family 17

    3. XM_006520555.2 → XP_006520618.1  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X2

      See identical proteins and their annotated locations for XP_006520618.1

      UniProtKB/Swiss-Prot
      P70386, Q10470
      UniProtKB/TrEMBL
      Q5RKT9
      Conserved Domains (1) summary
      pfam04724
      Location:205 → 502
      Glyco_transf_17; Glycosyltransferase family 17

    4. XM_006520553.2 → XP_006520616.1  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1

      UniProtKB/Swiss-Prot
      P70386, Q10470
      Conserved Domains (1) summary
      pfam04724
      Location:213 → 510
      Glyco_transf_17; Glycosyltransferase family 17

    5. XM_006520557.5 → XP_006520620.1  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X2

      See identical proteins and their annotated locations for XP_006520620.1

      UniProtKB/Swiss-Prot
      P70386, Q10470
      UniProtKB/TrEMBL
      Q5RKT9
      Conserved Domains (1) summary
      pfam04724
      Location:205 → 502
      Glyco_transf_17; Glycosyltransferase family 17

    6. XM_006520554.5 → XP_006520617.1  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X2

      See identical proteins and their annotated locations for XP_006520617.1

      UniProtKB/Swiss-Prot
      P70386, Q10470
      UniProtKB/TrEMBL
      Q5RKT9
      Conserved Domains (1) summary
      pfam04724
      Location:205 → 502
      Glyco_transf_17; Glycosyltransferase family 17

    RNA

    1. XR_004938806.1 RNA Sequence

    2. XR_004938807.1 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q10470.2 GenPept UniProtKB/Swiss-Prot:Q10470

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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