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    Mfng MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase [ Mus musculus (house mouse) ]

    Gene ID: 17305, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Mfngprovided by MGI
    Official Full Name
    MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferaseprovided by MGI
    Primary source
    MGI:MGI:1095404
    See related
    Ensembl:ENSMUSG00000018169 AllianceGenome:MGI:1095404
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Summary
    Predicted to enable O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity. Involved in marginal zone B cell differentiation and regulation of Notch signaling pathway. Acts upstream of or within blastocyst formation; positive regulation of Notch signaling pathway; and positive regulation of protein binding activity. Predicted to be located in Golgi apparatus and membrane. Predicted to be integral component of Golgi membrane. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; peripheral nervous system; and sensory organ. Orthologous to human MFNG (MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in subcutaneous fat pad adult (RPKM 50.0), mammary gland adult (RPKM 49.8) and 17 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    15 E1; 15 37.69 cM
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 15 NC_000081.7 (78640082..78657678, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 15 NC_000081.6 (78755882..78773516, complement)

    Chromosome 15 - NC_000081.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 1700041B01 gene Neighboring gene leucine rich repeat and fibronectin type III, extracellular 2 Neighboring gene predicted gene, 52188 Neighboring gene STARR-positive B cell enhancer ABC_E5495 Neighboring gene caspase recruitment domain family, member 10 Neighboring gene transmembrane emp24 domain trafficking protein 2 pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Structural insights into the Notch-modifying glycosyltransferase Fringe. Jinek M, et al. Nat Struct Mol Biol, 2006 Oct. PMID 16964258
    2. Eukaryotic expression, purification, crystallization and preliminary X-ray analysis of murine Manic Fringe. Jinek M, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2006 Aug 1. PMID 16880554, Free PMC Article
    3. Lunatic, Manic, and Radical Fringe Each Promote T and B Cell Development. Song Y, et al. J Immunol, 2016 Jan 1. PMID 26608918, Free PMC Article
    4. Galactose differentially modulates lunatic and manic fringe effects on Delta1-induced NOTCH signaling. Hou X, et al. J Biol Chem, 2012 Jan 2. PMID 22081605, Free PMC Article
    5. Lunatic fringe, manic fringe, and radical fringe recognize similar specificity determinants in O-fucosylated epidermal growth factor-like repeats. Rampal R, et al. J Biol Chem, 2005 Dec 23. PMID 16221665

    See all (65) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. MFNG imposes a negative correlation between Jag1 and Notch, being high Jag1 in the absence of MFNG predictive of poor prognosis.
      Title: Manic Fringe deficiency imposes Jagged1 addiction to intestinal tumor cells.
    2. These results show that Notch signaling is finely calibrated in the cochlea via Lfng and Mfng to produce precisely tuned levels of signaling that first set the boundary of the organ of Corti and later regulate hair cell development.
      Title: Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates.
    3. Fringe modifications at EGF8 and EGF12 enhanced Notch1 binding to and activation from Delta-like 1, while modifications at EGF6 and EGF36 (added by Manic and Lunatic but not Radical) inhibited Notch1 activation from Jagged1.
      Title: Deciphering the Fringe-Mediated Notch Code: Identification of Activating and Inhibiting Sites Allowing Discrimination between Ligands.
    4. each Fringe contributes to T and B cell development, and Fringe is required for optimal in vitro stimulation of T and B cells.
      Title: Lunatic, Manic, and Radical Fringe Each Promote T and B Cell Development.
    5. the presence of Gal on O-fucose glycans differentially affects DLL1-induced NOTCH signaling modulated by LFNG versus MFNG
      Title: Galactose differentially modulates lunatic and manic fringe effects on Delta1-induced NOTCH signaling.
    6. Manic fringe is not required for embryonic development.
      Title: Manic fringe is not required for embryonic development, and fringe family members do not exhibit redundant functions in the axial skeleton, limb, or hindbrain.
    7. MFng colocalized with the proendocrine transcription factor Ngn3 in the developing mouse pancreas between embryonic days 9 and 14
      Title: MFng is dispensable for mouse pancreas development and function.
    8. Lunatic Fringe (Lfng) and Manic Fringe (Mfng) cooperatively enhanced the DL1-Notch2 interaction to promote marginal zone B cell development
      Title: Lunatic and manic fringe cooperatively enhance marginal zone B cell precursor competition for delta-like 1 in splenic endothelial niches.
    9. Jag1/fringe genes (Lfng, Rfng, and Mfng) may regulate postnatal bile duct growth and remodeling, and serve as candidate modifiers of the hepatic phenotype in Alagille syndrome.
      Title: Bile duct proliferation in Jag1/fringe heterozygous mice identifies candidate modifiers of the Alagille syndrome hepatic phenotype.
    10. The structure of Manic Fringe reveals amino acid residues involved in recognition of donor substrates and catalysis, and a putative binding pocket for acceptor substrates. Mutations of several residues in this pocket impair Fringe activity in vivo.
      Title: Structural insights into the Notch-modifying glycosyltransferase Fringe.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Gene trapped (1) 
    • Targeted (6)  1 citation
    • Endonuclease-mediated (3) 

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables glycosyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within blastocyst formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in marginal zone B cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within multicellular organism development IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in pattern specification process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of Notch signaling pathway IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of Notch signaling pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of Notch signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in Golgi apparatus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in Golgi membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    beta-1,3-N-acetylglucosaminyltransferase manic fringe
    Names
    O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
    manic fringe homolog
    NP_001398307.1
    NP_032621.1
    XP_006520614.1
    XP_006520615.1
    XP_036015108.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001411378.1 → NP_001398307.1  beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform 2

      Status: VALIDATED

      Source sequence(s)
      AL591946, AL592169

    2. NM_008595.3 → NP_032621.1  beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform 1

      See identical proteins and their annotated locations for NP_032621.1

      Status: VALIDATED

      Source sequence(s)
      AL591946, AL592169
      Consensus CDS
      CCDS27622.1
      UniProtKB/Swiss-Prot
      O09008
      UniProtKB/TrEMBL
      Q3UPI0
      Related
      ENSMUSP00000018313.5, ENSMUST00000018313.6
      Conserved Domains (1) summary
      pfam02434
      Location:51 → 300
      Fringe; Fringe-like

    RNA

    1. NR_177237.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL591946, AL592169

    2. NR_177238.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL591946, AL592169

    3. NR_177239.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL591946, AL592169

    4. NR_177240.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL591946, AL592169

    5. NR_177241.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL591946, AL592169

    6. NR_177242.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL591946, AL592169

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000081.7 Reference GRCm39 C57BL/6J

      Range
      78640082..78657678 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006520551.3 → XP_006520614.1  beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2

      See identical proteins and their annotated locations for XP_006520614.1

      Conserved Domains (1) summary
      pfam02434
      Location:1 → 178
      Fringe; Fringe-like

    2. XM_036159215.1 → XP_036015108.1  beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2

      Conserved Domains (1) summary
      pfam02434
      Location:1 → 178
      Fringe; Fringe-like

    3. XM_006520552.4 → XP_006520615.1  beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2

      See identical proteins and their annotated locations for XP_006520615.1

      Conserved Domains (1) summary
      pfam02434
      Location:1 → 178
      Fringe; Fringe-like
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O09008.1 GenPept UniProtKB/Swiss-Prot:O09008

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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