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    Mfge8 milk fat globule EGF and factor V/VIII domain containing [ Mus musculus (house mouse) ]

    Gene ID: 17304, updated on 22-Apr-2024

    Summary

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    Official Symbol
    Mfge8provided by MGI
    Official Full Name
    milk fat globule EGF and factor V/VIII domain containingprovided by MGI
    Primary source
    MGI:MGI:102768
    See related
    Ensembl:ENSMUSG00000030605 AllianceGenome:MGI:102768
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    P47; MP47; Mfgm; SED1; MFG-E8; lactadherin
    Summary
    Enables integrin binding activity; phosphatidylethanolamine binding activity; and phosphatidylserine binding activity. Involved in apoptotic cell clearance. Acts upstream of or within phagocytosis, engulfment; phagocytosis, recognition; and positive regulation of phagocytosis. Located in external side of plasma membrane and extracellular space. Is extrinsic component of plasma membrane. Is expressed in several structures, including alimentary system; cap mesenchyme; central nervous system; reproductive system; and sensory organ. Human ortholog(s) of this gene implicated in temporal arteritis. Orthologous to human MFGE8 (milk fat globule EGF and factor V/VIII domain containing). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in genital fat pad adult (RPKM 1673.1), spleen adult (RPKM 373.7) and 7 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    7 D2; 7 44.9 cM
    Exon count:
    10
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (78783516..78798808, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (79133768..79149060, complement)

    Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 26633 Neighboring gene aggrecan Neighboring gene STARR-seq mESC enhancer starr_19258 Neighboring gene hyaluronan and proteoglycan link protein 3 Neighboring gene STARR-seq mESC enhancer starr_19263 Neighboring gene STARR-seq mESC enhancer starr_19264 Neighboring gene predicted gene, 39040 Neighboring gene STARR-seq mESC enhancer starr_19267 Neighboring gene STARR-positive B cell enhancer ABC_E47 Neighboring gene predicted gene, 31510

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. MFG-E8 Knockout Aggravated Nonalcoholic Steatohepatitis by Promoting the Activation of TLR4/NF-κB Signaling in Mice. Hu J, et al. Mediators Inflamm, 2022. PMID 35769208, Free PMC Article
    2. MFG-E8 Reduces Aortic Intimal Proliferation in a Murine Model of Transplant Vasculopathy. Brilland B, et al. Int J Mol Sci, 2022 Apr 7. PMID 35456911, Free PMC Article
    3. MFG-E8 promotes osteogenic transdifferentiation of smooth muscle cells and vascular calcification by regulating TGF-β1 signaling. Chiang HY, et al. Commun Biol, 2022 Apr 19. PMID 35440618, Free PMC Article
    4. Protective Effect of MFG-E8 on Necroptosis-Induced Intestinal Inflammation and Enteroendocrine Cell Function in Diabetes. Hua X, et al. Nutrients, 2022 Jan 29. PMID 35276962, Free PMC Article
    5. MFG-E8 Maintains Cellular Homeostasis by Suppressing Endoplasmic Reticulum Stress in Pancreatic Exocrine Acinar Cells. Ren Y, et al. Front Cell Dev Biol, 2021. PMID 35096831, Free PMC Article

    See all (192) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. MFG-E8 Alleviates Cognitive Impairments Induced by Chronic Cerebral Hypoperfusion by Phagocytosing Myelin Debris and Promoting Remyelination.
      Title: MFG-E8 Alleviates Cognitive Impairments Induced by Chronic Cerebral Hypoperfusion by Phagocytosing Myelin Debris and Promoting Remyelination.
    2. PTP1B mediates the inhibitory effect of MFGE8 on insulin signaling through the beta5 integrin.
      Title: PTP1B mediates the inhibitory effect of MFGE8 on insulin signaling through the β5 integrin.
    3. MFG-E8 facilitates heart repair through M1/M2 polarization after myocardial infarction by inhibiting CaMKII.
      Title: MFG-E8 facilitates heart repair through M1/M2 polarization after myocardial infarction by inhibiting CaMKII.
    4. hMSCs-derived exosome circCDK13 inhibits liver fibrosis by regulating the expression of MFGE8 through miR-17-5p/KAT2B.
      Title: hMSCs-derived exosome circCDK13 inhibits liver fibrosis by regulating the expression of MFGE8 through miR-17-5p/KAT2B.
    5. Medin co-aggregates with vascular amyloid-beta in Alzheimer's disease.
      Title: Medin co-aggregates with vascular amyloid-β in Alzheimer's disease.
    6. MFG-E8 has guiding significance for the prognosis and treatment of sepsis.
      Title: MFG-E8 has guiding significance for the prognosis and treatment of sepsis.
    7. MicroRNA-34b-5p binds enhancer of zeste 2 to inhibit milk fat globule-EGF factor 8 expression, affecting liver fibrosis.
      Title: MicroRNA-34b-5p binds enhancer of zeste 2 to inhibit milk fat globule-EGF factor 8 expression, affecting liver fibrosis.
    8. MFG-E8 Knockout Aggravated Nonalcoholic Steatohepatitis by Promoting the Activation of TLR4/NF-kappaB Signaling in Mice.
      Title: MFG-E8 Knockout Aggravated Nonalcoholic Steatohepatitis by Promoting the Activation of TLR4/NF-κB Signaling in Mice.
    9. MFG-E8 Reduces Aortic Intimal Proliferation in a Murine Model of Transplant Vasculopathy.
      Title: MFG-E8 Reduces Aortic Intimal Proliferation in a Murine Model of Transplant Vasculopathy.
    10. Increased MFG-E8 at neuromuscular junctions is an exacerbating factor for sarcopenia-associated denervation.
      Title: Increased MFG-E8 at neuromuscular junctions is an exacerbating factor for sarcopenia-associated denervation.
    Submit: New GeneRIF Correction See all GeneRIFs (116)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables extracellular matrix structural constituent RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    enables integrin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables phosphatidylserine binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within angiogenesis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in apoptotic cell clearance IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within cell adhesion IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within single fertilization IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in collagen-containing extracellular matrix HDA PubMed 
    located_in cytoplasmic vesicle IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in external side of plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in extracellular region IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in extracellular space HDA PubMed 
    located_in extracellular space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    lactadherin
    Names
    EGF/factor VIII
    milk fat globule glycoprotein
    milk fat globule-EGF factor 8 protein
    sperm surface protein SP47

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001045489.1 → NP_001038954.1  lactadherin isoform 2 precursor

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an alternate exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.
      Source sequence(s)
      AC110520, AK087555, AK148371, CD776821
      Consensus CDS
      CCDS39989.1
      UniProtKB/TrEMBL
      Q3TDU5
      Related
      ENSMUSP00000103032.4, ENSMUST00000107409.5
      Conserved Domains (2) summary
      cd00057
      Location:273 → 425
      FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain; Cell surface-attached carbohydrate-binding domain, present in eukaryotes and assumed to have horizontally transferred to eubacterial genomes.
      pfam00008
      Location:68 → 106
      EGF; EGF-like domain

    2. NM_008594.2 → NP_032620.2  lactadherin isoform 1 precursor

      See identical proteins and their annotated locations for NP_032620.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).
      Source sequence(s)
      AC110520, AK152088, CD776821
      Consensus CDS
      CCDS21379.1
      UniProtKB/Swiss-Prot
      P21956, P97800, Q3TBN5, Q3U8S9, Q9R1X9, Q9WTS3
      UniProtKB/TrEMBL
      Q3TDU5
      Related
      ENSMUSP00000032825.9, ENSMUST00000032825.14
      Conserved Domains (3) summary
      smart00231
      Location:307 → 463
      FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain
      cd00057
      Location:310 → 462
      FA58C; Coagulation factor 5/8 C-terminal domain, discoidin domain; Cell surface-attached carbohydrate-binding domain, present in eukaryotes and assumed to have horizontally transferred to eubacterial genomes.
      pfam00008
      Location:68 → 106
      EGF; EGF-like domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000073.7 Reference GRCm39 C57BL/6J

      Range
      78783516..78798808 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P21956.3 GenPept UniProtKB/Swiss-Prot:P21956

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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