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    Mcm3 minichromosome maintenance complex component 3 [ Mus musculus (house mouse) ]

    Gene ID: 17215, updated on 5-Mar-2024

    Summary

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    Official Symbol
    Mcm3provided by MGI
    Official Full Name
    minichromosome maintenance complex component 3provided by MGI
    Primary source
    MGI:MGI:101845
    See related
    Ensembl:ENSMUSG00000041859 AllianceGenome:MGI:101845
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    P1; Mcmd; p1.m; P1-MCM3
    Summary
    Predicted to enable single-stranded DNA binding activity. Predicted to contribute to single-stranded DNA helicase activity. Predicted to be involved in DNA strand elongation involved in DNA replication; double-strand break repair via break-induced replication; and mitotic DNA replication initiation. Located in cytoplasm and nucleus. Is expressed in several structures, including brain; limb; myocardium layer; neural ectoderm; and retina. Orthologous to human MCM3 (minichromosome maintenance complex component 3). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in liver E14 (RPKM 48.6), liver E14.5 (RPKM 44.8) and 18 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Mcm3 in Genome Data Viewer
    Location:
    1 A4; 1 6.5 cM
    Exon count:
    17
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (20873188..20890536, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (20802966..20820312, complement)

    Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene interleukin 17A Neighboring gene STARR-seq mESC enhancer starr_00390 Neighboring gene interleukin 17F Neighboring gene STARR-positive B cell enhancer ABC_E1536 Neighboring gene chaperonin containing Tcp1, subunit 7 (eta) pseudogene Neighboring gene predicted gene, 24723

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. The murine replication protein P1 is differentially expressed during spermatogenesis. Starborg M, et al. Eur J Cell Biol, 1995 Oct. PMID 8575467
    2. DNA polymerase alpha associated protein P1, a murine homolog of yeast MCM3, changes its intranuclear distribution during the DNA synthetic period. Kimura H, et al. EMBO J, 1994 Sep 15. PMID 7925275, Free PMC Article
    3. Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex. Lin DI, et al. Proc Natl Acad Sci U S A, 2008 Jun 10. PMID 18524952, Free PMC Article
    4. Mouse pre-replicative complex proteins colocalise and interact with the centrosome. Stuermer A, et al. Eur J Cell Biol, 2007 Jan. PMID 17157410
    5. A murine replication protein accumulates temporarily in the heterochromatic regions of nuclei prior to initiation of DNA replication. Starborg M, et al. J Cell Sci, 1995 Mar. PMID 7622621

    See all (71) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Study characterize the interaction of Keap1, a central antioxidant response regulator in Metazoa, with the replicative helicase subunit protein MCM3. Analysis suggests that structural determinants of the interaction of Keap1 with its critical downstream target - Nrf2 master transactivator of oxidative stress response genes - may have evolved in evolution to mimic the conserved helix-2-insert motif of MCM3.
      Title: Keap1-MCM3 interaction is a potential coordinator of molecular machineries of antioxidant response and genomic DNA replication in metazoa.
    2. Mcm3-deficient erythroblasts display aberrant DNA replication patterns and fail to complete maturation, causing lethal anemia.
      Title: Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality.
    3. These data reveal that CDK-dependent MCM3 phosphorylation contributes to the regulated formation of the MCM2-7 complex.
      Title: Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex.
    4. Depletion of Orc2 and Mcm3 by siRNA leads to an inhibition of cell proliferation, an altered cell cycle distribution as well as to multinucleated cells with insufficiently organised microtubules.
      Title: Mouse pre-replicative complex proteins colocalise and interact with the centrosome.
    5. interaction with GANP DNA-primase is associated with a novel phosphatase component G5PR
      Title: MCM3-binding GANP DNA-primase is associated with a novel phosphatase component G5PR.
    6. analysis of carboxyl-terminal MCM3 phosphorylation sites and their motifs
      Title: Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables helicase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables single-stranded DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    contributes_to single-stranded DNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA duplex unwinding IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication initiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA strand elongation involved in DNA replication IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA unwinding involved in DNA replication ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within cell cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in double-strand break repair via break-induced replication IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in mitotic DNA replication initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in premeiotic DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of CMG complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    part_of MCM complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of MCM complex ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in centrosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in chromosome IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in perinuclear region of cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    DNA replication licensing factor MCM3
    Names
    DNA polymerase alpha holoenzyme-associated protein P1
    mini chromosome maintenance deficient
    minichromosome maintenance deficient 3
    NP_032589.1
    XP_030107786.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_008563.3 → NP_032589.1  DNA replication licensing factor MCM3

      See identical proteins and their annotated locations for NP_032589.1

      Status: VALIDATED

      Source sequence(s)
      AC156980, AC159614
      Consensus CDS
      CCDS14843.1
      UniProtKB/Swiss-Prot
      P25206, Q61492
      UniProtKB/TrEMBL
      Q3UI57, Q3ULD6, Q3UZH2
      Related
      ENSMUSP00000059192.10, ENSMUST00000053266.11
      Conserved Domains (3) summary
      smart00350
      Location:109 → 654
      MCM; minichromosome maintenance proteins
      pfam14551
      Location:17 → 129
      MCM_N; MCM N-terminal domain
      cl21455
      Location:340 → 479
      P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000067.7 Reference GRCm39 C57BL/6J

      Range
      20873188..20890536 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030251926.2 → XP_030107786.1  DNA replication licensing factor MCM3 isoform X1

      UniProtKB/TrEMBL
      Q3UZH2
      Conserved Domains (1) summary
      smart00350
      Location:63 → 608
      MCM; minichromosome maintenance proteins
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P25206.2 GenPept UniProtKB/Swiss-Prot:P25206

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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