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    Usp33 ubiquitin specific peptidase 33 [ Mus musculus (house mouse) ]

    Gene ID: 170822, updated on 22-Apr-2024

    Summary

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    Official Symbol
    Usp33provided by MGI
    Official Full Name
    ubiquitin specific peptidase 33provided by MGI
    Primary source
    MGI:MGI:2159711
    See related
    Ensembl:ENSMUSG00000025437 AllianceGenome:MGI:2159711
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Vdu1; 9830169D19Rik
    Summary
    Predicted to enable several functions, including cysteine-type peptidase activity; small GTPase binding activity; and zinc ion binding activity. Involved in axon guidance and cell migration. Located in cell body. Is expressed in central nervous system and retina. Orthologous to human USP33 (ubiquitin specific peptidase 33). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in CNS E18 (RPKM 26.3), CNS E14 (RPKM 24.1) and 25 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    3 H3; 3 77.15 cM
    Exon count:
    25
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 3 NC_000069.7 (152051797..152099254)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 3 NC_000069.6 (152346184..152393617)

    Chromosome 3 - NC_000069.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 31999 Neighboring gene VISTA enhancer mm72 Neighboring gene mitoguardin 1 Neighboring gene zinc finger, ZZ domain containing 3 Neighboring gene predicted gene, 57837 Neighboring gene RIKEN cDNA 3110067G11 gene Neighboring gene STARR-positive B cell enhancer ABC_E4606 Neighboring gene adenylate kinase 5

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Midline crossing and Slit responsiveness of commissural axons require USP33. Yuasa-Kawada J, et al. Nat Neurosci, 2009 Sep. PMID 19684588, Free PMC Article
    2. The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination. Cubillos-Rojas M, et al. Oncotarget, 2016 Aug 30. PMID 27528230, Free PMC Article
    3. Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2. Chan NC, et al. J Biol Chem, 2014 Jul 11. PMID 24855649, Free PMC Article
    4. Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein. Li Z, et al. J Biol Chem, 2002 Feb 15. PMID 11739384
    5. Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration. Yuasa-Kawada J, et al. Proc Natl Acad Sci U S A, 2009 Aug 25. PMID 19706539, Free PMC Article

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway.
      Title: miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway.
    2. Knockdown or chemical inhibition of p97 causes robust accumulation of USP33 due to inhibition of its degradation
      Title: Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2.
    3. Our results reveal a previously unknown role for USP33 in vertebrate commissural axon guidance and in Slit signaling
      Title: Midline crossing and Slit responsiveness of commissural axons require USP33.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (1) 
    • Endonuclease-mediated (4) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables G protein-coupled receptor binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables cysteine-type deubiquitinase activity ISO
    Inferred from Sequence Orthology
    more info
    		 PubMed 
    enables cysteine-type endopeptidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables cysteine-type peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables small GTPase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables zinc ion binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in axon guidance IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cell migration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to starvation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in centrosome duplication ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within endocytosis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein K48-linked deubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein K63-linked deubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein deubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein stabilization ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of G protein-coupled receptor signaling pathway ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of autophagy ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi apparatus ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in centrosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in focal adhesion ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in neuronal cell body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in perinuclear region of cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    ubiquitin carboxyl-terminal hydrolase 33
    Names
    VHL-interacting deubiquitinating enzyme 1
    Vhlh-interacting deubiquitinating enzyme 1
    deubiquitinating enzyme 33
    pVHL-interacting deubiquitinating enzyme 1
    ubiquitin specific protease 33
    ubiquitin thioesterase 33
    ubiquitin thiolesterase 33
    ubiquitin-specific-processing protease 33
    NP_001070144.1
    NP_001239415.1
    NP_001342595.1
    NP_573510.2
    XP_030108303.1
    XP_030108304.1
    XP_036018806.1
    XP_036018807.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001076676.2 → NP_001070144.1  ubiquitin carboxyl-terminal hydrolase 33 isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses two alternate in-frame splice junctions compared to variant 1, resulting in a shorter protein (isoform 2).
      Source sequence(s)
      AA210301, AK082943, AK087467
      Consensus CDS
      CCDS80064.1
      UniProtKB/TrEMBL
      A0A0H2UKB8, Q80TK2
      Related
      ENSMUSP00000026507.8, ENSMUST00000026507.13
      Conserved Domains (6) summary
      smart00695
      Location:799 → 879
      DUSP; Domain in ubiquitin-specific proteases
      cd02674
      Location:441 → 672
      Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      cl02553
      Location:155 → 287
      Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...
      pfam00443
      Location:153 → 671
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam02148
      Location:30 → 90
      zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
      cl12116
      Location:693 → 762
      DUSP; DUSP domain

    2. NM_001252486.1 → NP_001239415.1  ubiquitin carboxyl-terminal hydrolase 33 isoform 3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1, resulting in a shorter protein (isoform 3).
      Source sequence(s)
      AA210301, AK087467, AK143844, BC089315
      Consensus CDS
      CCDS57261.1
      UniProtKB/TrEMBL
      Q80TK2
      Related
      ENSMUSP00000113265.4, ENSMUST00000117492.9
      Conserved Domains (6) summary
      smart00695
      Location:799 → 883
      DUSP; Domain in ubiquitin-specific proteases
      cd02674
      Location:441 → 672
      Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      cl02553
      Location:155 → 287
      Peptidase_C19; Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyse bonds involving the carboxyl group of the C-terminal Gly ...
      pfam00443
      Location:153 → 671
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam02148
      Location:30 → 90
      zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
      cl12116
      Location:693 → 762
      DUSP; DUSP domain

    3. NM_001355666.1 → NP_001342595.1  ubiquitin carboxyl-terminal hydrolase 33 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AA210301, AC122529
      UniProtKB/TrEMBL
      Q80TK2
      Conserved Domains (3) summary
      pfam00443
      Location:154 → 679
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam02148
      Location:30 → 90
      zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
      cl12116
      Location:701 → 764
      DUSP; DUSP domain

    4. NM_133247.3 → NP_573510.2  ubiquitin carboxyl-terminal hydrolase 33 isoform 1

      See identical proteins and their annotated locations for NP_573510.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
      Source sequence(s)
      AA210301, AK087467, AK143844, BC089315
      Consensus CDS
      CCDS17918.1
      UniProtKB/Swiss-Prot
      Q3UP32, Q5FWK0, Q8K0I3, Q8R5K2, Q99K22
      UniProtKB/TrEMBL
      Q80TK2
      Related
      ENSMUSP00000142708.2, ENSMUST00000197748.5
      Conserved Domains (4) summary
      smart00695
      Location:807 → 891
      DUSP; Domain in ubiquitin-specific proteases
      pfam00443
      Location:154 → 679
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam02148
      Location:30 → 90
      zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
      cl12116
      Location:701 → 770
      DUSP; DUSP domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000069.7 Reference GRCm39 C57BL/6J

      Range
      152051797..152099254
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036162913.1 → XP_036018806.1  ubiquitin carboxyl-terminal hydrolase 33 isoform X2

      UniProtKB/TrEMBL
      Q80TK2
      Conserved Domains (3) summary
      pfam00443
      Location:154 → 679
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam02148
      Location:30 → 90
      zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
      cl12116
      Location:701 → 770
      DUSP; DUSP domain

    2. XM_036162914.1 → XP_036018807.1  ubiquitin carboxyl-terminal hydrolase 33 isoform X3

      UniProtKB/TrEMBL
      Q80TK2
      Conserved Domains (3) summary
      pfam00443
      Location:154 → 671
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam02148
      Location:30 → 90
      zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
      cl12116
      Location:693 → 762
      DUSP; DUSP domain

    3. XM_030252444.2 → XP_030108304.1  ubiquitin carboxyl-terminal hydrolase 33 isoform X4

      UniProtKB/TrEMBL
      Q80TK2
      Conserved Domains (3) summary
      pfam00443
      Location:154 → 671
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam02148
      Location:30 → 90
      zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
      cl12116
      Location:693 → 756
      DUSP; DUSP domain

    4. XM_030252443.2 → XP_030108303.1  ubiquitin carboxyl-terminal hydrolase 33 isoform X1

      UniProtKB/Swiss-Prot
      Q3UP32, Q5FWK0, Q8K0I3, Q8R5K2, Q99K22
      UniProtKB/TrEMBL
      Q80TK2
      Conserved Domains (4) summary
      smart00695
      Location:807 → 891
      DUSP; Domain in ubiquitin-specific proteases
      pfam00443
      Location:154 → 679
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam02148
      Location:30 → 90
      zf-UBP; Zn-finger in ubiquitin-hydrolases and other protein
      cl12116
      Location:701 → 770
      DUSP; DUSP domain
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q8R5K2.2 GenPept UniProtKB/Swiss-Prot:Q8R5K2

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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