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    Capn8 calpain 8 [ Mus musculus (house mouse) ]

    Gene ID: 170725, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Capn8provided by MGI
    Official Full Name
    calpain 8provided by MGI
    Primary source
    MGI:MGI:2181366
    See related
    Ensembl:ENSMUSG00000038599 AllianceGenome:MGI:2181366
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    nCL-2; nCL-2'
    Summary
    Enables calcium-dependent cysteine-type endopeptidase activity and identical protein binding activity. Acts upstream of or within calcium-dependent self proteolysis. Predicted to be located in Golgi apparatus. Predicted to be active in cytoplasm. Is expressed in gut; liver; and male reproductive gland or organ. Orthologous to human CAPN8 (calpain 8). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Biased expression in stomach adult (RPKM 4.6), colon adult (RPKM 1.6) and 6 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    1 H5; 1 85.37 cM
    Exon count:
    22
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (182392314..182462021)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (182564749..182634456)

    Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 46081 Neighboring gene GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus pseudogene Neighboring gene predicted gene, 46248 Neighboring gene coiled-coil domain containing 185 Neighboring gene sushi domain containing 4 Neighboring gene STARR-seq mESC enhancer starr_03290 Neighboring gene STARR-seq mESC enhancer starr_03291

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. A Gastrointestinal Calpain Complex, G-calpain, Is a Heterodimer of CAPN8 and CAPN9 Calpain Isoforms, Which Play Catalytic and Regulatory Roles, Respectively. Hata S, et al. J Biol Chem, 2016 Dec 30. PMID 27881674, Free PMC Article
    2. Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense. Hata S, et al. PLoS Genet, 2010 Jul 29. PMID 20686710, Free PMC Article
    3. Both the conserved and the unique gene structure of stomach-specific calpains reveal processes of calpain gene evolution. Hata S, et al. J Mol Evol, 2001 Sep. PMID 11523006
    4. Mouse population-guided resequencing reveals that variants in CD44 contribute to acetaminophen-induced liver injury in humans. Harrill AH, et al. Genome Res, 2009 Sep. PMID 19416960, Free PMC Article
    5. Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP. Hata S, et al. J Biol Chem, 2006 Apr 21. PMID 16476741

    See all (18) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. gastrointestinal-tract-specific calpains 8 and 9 formed a complex we termed "G-calpain," in which both were essential for gastric mucosal defense
      Title: Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense.
    2. nCL-2/calpain 8 is active without calpain regulatory subunit and oligomerizes through C2-like domains
      Title: Stomach-specific calpain, nCL-2/calpain 8, is active without calpain regulatory subunit and oligomerizes through C2-like domains.
    3. nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP
      Title: Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (6)  1 citation
    • Endonuclease-mediated (1) 

    General gene information

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    Homology

    Clone Names

    • MGC159207, MGC159211

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables calcium-dependent cysteine-type endopeptidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables calcium-dependent cysteine-type endopeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables calcium-dependent cysteine-type endopeptidase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables cysteine-type peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within calcium-dependent self proteolysis IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in proteolysis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in Golgi apparatus IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    calpain-8
    Names
    new calpain 2
    stomach-specific M-type calpain
    stomach-specific calpain (nCL-2)
    NP_001139278.1
    NP_570960.2
    XP_006496749.1
    XP_017174541.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001145806.1 → NP_001139278.1  calpain-8 isoform 2

      See identical proteins and their annotated locations for NP_001139278.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1.
      Source sequence(s)
      AB050203, AK132449
      Consensus CDS
      CCDS48477.1
      UniProtKB/Swiss-Prot
      Q91VA3
      Related
      ENSMUSP00000129549.2, ENSMUST00000168514.7
      Conserved Domains (2) summary
      pfam00648
      Location:46 → 342
      Peptidase_C2; Calpain family cysteine protease
      cl00165
      Location:356 → 381
      Calpain_III; Calpain, subdomain III. Calpains are calcium-activated cytoplasmic cysteine proteinases, participate in cytoskeletal remodeling processes, cell differentiation, apoptosis and signal transduction. Catalytic domain and the two calmodulin-like domains are ...

    2. NM_130890.2 → NP_570960.2  calpain-8 isoform 1

      See identical proteins and their annotated locations for NP_570960.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longer isoform (1).
      Source sequence(s)
      AB050202, AC123793, AK132449, BC125504
      Consensus CDS
      CCDS35814.1
      UniProtKB/Swiss-Prot
      Q059W1, Q91UZ9, Q91VA3, Q920R7
      UniProtKB/TrEMBL
      A0A0R4J0K6
      Related
      ENSMUSP00000047164.8, ENSMUST00000048941.14
      Conserved Domains (4) summary
      PTZ00183
      Location:611 → 692
      PTZ00183; centrin; Provisional
      pfam00648
      Location:46 → 342
      Peptidase_C2; Calpain family cysteine protease
      pfam01067
      Location:356 → 509
      Calpain_III; Calpain large subunit, domain III
      cl08302
      Location:580 → 635
      EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000067.7 Reference GRCm39 C57BL/6J

      Range
      182392314..182462021
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_017319052.1 → XP_017174541.1  calpain-8 isoform X1

      UniProtKB/TrEMBL
      A0A0A6YVU9
      Conserved Domains (4) summary
      cd00214
      Location:374 → 534
      Calpain_III; Calpain, subdomain III. Calpains are calcium-activated cytoplasmic cysteine proteinases, participate in cytoskeletal remodeling processes, cell differentiation, apoptosis and signal transduction. Catalytic domain and the two calmodulin-like domains are ...
      cd15897
      Location:557 → 584
      EFh_PEF; EF-hand motif [structural motif]
      cl25352
      Location:557 → 659
      EFh_PEF; The penta-EF hand (PEF) family
      pfam00648
      Location:66 → 362
      Peptidase_C2; Calpain family cysteine protease

    2. XM_006496686.3 → XP_006496749.1  calpain-8 isoform X2

      UniProtKB/Swiss-Prot
      Q059W1, Q91UZ9, Q91VA3, Q920R7
      Conserved Domains (4) summary
      cd00214
      Location:374 → 534
      Calpain_III; Calpain, subdomain III. Calpains are calcium-activated cytoplasmic cysteine proteinases, participate in cytoskeletal remodeling processes, cell differentiation, apoptosis and signal transduction. Catalytic domain and the two calmodulin-like domains are ...
      cd15897
      Location:557 → 584
      EFh_PEF; EF-hand motif [structural motif]
      cl25352
      Location:557 → 672
      EFh_PEF; The penta-EF hand (PEF) family
      pfam00648
      Location:66 → 362
      Peptidase_C2; Calpain family cysteine protease

    RNA

    1. XR_373596.3 RNA Sequence

    2. XR_004940678.1 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q91VA3.1 GenPept UniProtKB/Swiss-Prot:Q91VA3

    Gene LinkOut

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