Ilk integrin linked kinase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ilkprovided by MGI
Official Full Name: integrin linked kinaseprovided by MGI
Primary source: MGI:MGI:1195267
See related: Ensembl:ENSMUSG00000030890 AllianceGenome:MGI:1195267
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: ILK-1; ILK-2; ESTM24; p59ILK
Summary: Predicted to enable several functions, including SH3 domain binding activity; integrin binding activity; and protein kinase binding activity. Involved in outflow tract morphogenesis; positive regulation of BMP signaling pathway; and positive regulation of osteoblast differentiation. Acts upstream of or within several processes, including fibroblast migration; nervous system development; and protein kinase B signaling. Located in focal adhesion and lamellipodium. Is expressed in several structures, including adipose tissue; alimentary system; brain; genitourinary system; and retina. Used to study dilated cardiomyopathy. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to human ILK (integrin linked kinase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in bladder adult (RPKM 143.4), subcutaneous fat pad adult (RPKM 90.0) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 E3; 7 55.97 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (105385797..105392132)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (105736590..105742925)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ILK inhibition reduces osteophyte formation through suppression of osteogenesis in BMSCs via Akt/GSK-3beta/beta-catenin pathway.
Title: ILK inhibition reduces osteophyte formation through suppression of osteogenesis in BMSCs via Akt/GSK-3β/β-catenin pathway.
Integrin-linked kinase expression in myeloid cells promotes colon tumorigenesis.
Title: Integrin-linked kinase expression in myeloid cells promotes colon tumorigenesis.
Mechanosensors control skeletal muscle mass, molecular clocks, and metabolism.
Title: Mechanosensors control skeletal muscle mass, molecular clocks, and metabolism.
Substratum stiffness signals through integrin-linked kinase and beta1-integrin to regulate midbody proteins and abscission during EMT.
Title: Substratum stiffness signals through integrin-linked kinase and β1-integrin to regulate midbody proteins and abscission during EMT.
Disruption of the integrin-linked kinase (ILK) pseudokinase domain affects kidney development in mice.
Title: Disruption of the integrin-linked kinase (ILK) pseudokinase domain affects kidney development in mice.
Integrin-linked kinase tunes cell-cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFbeta1.
Title: Integrin-linked kinase tunes cell-cell and cell-matrix adhesions to regulate the switch between apoptosis and EMT downstream of TGFβ1.
Integrin-linked kinase regulates melanosome trafficking and melanin transfer in melanocytes.
Title: Integrin-linked kinase regulates melanosome trafficking and melanin transfer in melanocytes.
Essential Role for Integrin-Linked Kinase in Melanoblast Colonization of the Skin.
Title: Essential Role for Integrin-Linked Kinase in Melanoblast Colonization of the Skin.
L1CAM-ILK-YAP Mechanotransduction Drives Proliferative Activity of Epithelial Cells in Middle Ear Cholesteatoma.
Title: L1CAM-ILK-YAP Mechanotransduction Drives Proliferative Activity of Epithelial Cells in Middle Ear Cholesteatoma.
ILK was highly expressed in unilateral ureteral obstruction (UUO) model in CD1 mice. Renal fibrosis was aggravated after injection of ILK overexpression plasmid in UUO mice. Western blot results showed that expressions of p-smad3 and smad3 were upregulated during renal fibrosis. ILK participates in the development of renal fibrosis by altering phenotypes of renal tubular epithelial cells via a TGF-beta1/smad pathway.
Title: ILK participates in renal interstitial fibrosis by altering the phenotype of renal tubular epithelial cells via TGF-β1/smad pathway.

Submit: New GeneRIF Correction See all GeneRIFs (134)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (15) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Ilk (e-PCR), detects polymorphism
- UniSTS:527395

AA511515 (e-PCR), detects polymorphism
- UniSTS:143010

NoName (e-PCR)
- UniSTS:235732

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables SH3 domain binding	ISO Inferred from Sequence Orthology more info
enables integrin binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within Schwann cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within branching involved in ureteric bud morphogenesis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cell morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell projection organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell-matrix adhesion	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within establishment or maintenance of epithelial cell apical/basal polarity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within fibroblast migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in integrin-mediated signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within integrin-mediated signaling pathway	ISO Inferred from Sequence Orthology more info	PubMed
involved_in integrin-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in myelin assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within myelination in peripheral nervous system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cardiac muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of neural precursor cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of neural precursor cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smooth muscle cell migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within nerve development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of neural precursor cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of neural precursor cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron projection morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in outflow tract morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within peptidyl-serine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phosphatidylinositol 3-kinase/protein kinase B signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of BMP signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of axon extension	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of axonogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell-matrix adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cellular senescence	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dendrite morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of myoblast differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of osteoblast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of substrate adhesion-dependent cell spreading	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in substrate adhesion-dependent cell spreading	IBA Inferred from Biological aspect of Ancestor more info
involved_in substrate adhesion-dependent cell spreading	ISO Inferred from Sequence Orthology more info
involved_in supramolecular fiber organization	ISO Inferred from Sequence Orthology more info
involved_in tumor necrosis factor-mediated signaling pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in anchoring junction	IEA Inferred from Electronic Annotation more info
located_in axon	ISO Inferred from Sequence Orthology more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
located_in costamere	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendritic shaft	ISO Inferred from Sequence Orthology more info
is_active_in focal adhesion	IBA Inferred from Biological aspect of Ancestor more info
located_in focal adhesion	IDA Inferred from Direct Assay more info	PubMed
located_in focal adhesion	ISO Inferred from Sequence Orthology more info	PubMed
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
colocalizes_with stress fiber	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with stress fiber	ISO Inferred from Sequence Orthology more info
located_in terminal bouton	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: integrin-linked protein kinase

Names: 59 kDa serine/threonine-protein kinase; beta-integrin-linked kinase; integrin binding protein kinase

NP_001155196.1

EC 2.7.11.1

NP_034692.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001161724.1 → NP_001155196.1 integrin-linked protein kinase

See identical proteins and their annotated locations for NP_001155196.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

Source sequence(s)

AC121823, AK002344, AK011766, CA752166, CK625497

Consensus CDS

CCDS21659.1

UniProtKB/Swiss-Prot

O55222, Q78KK2

Related

ENSMUSP00000033182.4, ENSMUST00000033182.10

Conserved Domains (4) summary

cd14057
Location:198 → 449

PK_ILK; Pseudokinase domain of Integrin Linked Kinase

sd00045
Location:33 → 64

ANK; ANK repeat [structural motif]

pfam13637
Location:4 → 54

Ank_4; Ankyrin repeats (many copies)

pfam12796
Location:38 → 124

Ank_2; Ankyrin repeats (3 copies)
NM_010562.2 → NP_034692.2 integrin-linked protein kinase

See identical proteins and their annotated locations for NP_034692.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.

Source sequence(s)

AC121823

Consensus CDS

CCDS21659.1

UniProtKB/Swiss-Prot

O55222, Q78KK2

Related

ENSMUSP00000130341.2, ENSMUST00000163389.9

Conserved Domains (4) summary

cd14057
Location:198 → 449

PK_ILK; Pseudokinase domain of Integrin Linked Kinase

sd00045
Location:33 → 64

ANK; ANK repeat [structural motif]

pfam13637
Location:4 → 54

Ank_4; Ankyrin repeats (many copies)

pfam12796
Location:38 → 124

Ank_2; Ankyrin repeats (3 copies)