Hoxb8 homeobox B8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Hoxb8provided by MGI
Official Full Name: homeobox B8provided by MGI
Primary source: MGI:MGI:96189
See related: Ensembl:ENSMUSG00000056648 AllianceGenome:MGI:96189
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Hox-2.4
Summary: Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and sequence-specific DNA binding activity. Involved in negative regulation of myeloid cell differentiation and negative regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including dorsal spinal cord development; embryonic skeletal system morphogenesis; and grooming behavior. Predicted to be located in nucleoplasm. Predicted to be active in nucleus. Is expressed in several structures, including embryo mesenchyme; extraembryonic component; genitourinary system; nervous system; and neural ectoderm. Used to study trichotillomania. Orthologous to human HOXB8 (homeobox B8). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in adrenal adult (RPKM 49.5), genital fat pad adult (RPKM 24.0) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 D; 11 59.82 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (96170227..96176151)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (96279401..96285325)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A Microglia Sublineage Protects from Sex-Linked Anxiety Symptoms and Obsessive Compulsion.
Title: A Microglia Sublineage Protects from Sex-Linked Anxiety Symptoms and Obsessive Compulsion.
Signaling and functional competency of neutrophils derived from bone-marrow cells expressing the ER-HOXB8 oncoprotein.
Title: Signaling and functional competency of neutrophils derived from bone-marrow cells expressing the ER-HOXB8 oncoprotein.
HoxB8 neutrophils represent a faithful experimental model system for the analysis of Fc and integrin receptor-dependent neutrophil functions.
Title: HoxB8 neutrophils replicate Fcγ receptor and integrin-induced neutrophil signaling and functions.
CRISPR/Cas9 Immunoengineering of Hoxb8-Immortalized Progenitor Cells for Revealing CCR7-Mediated Dendritic Cell Signaling and Migration Mechanisms in vivo.
Title: CRISPR/Cas9 Immunoengineering of Hoxb8-Immortalized Progenitor Cells for Revealing CCR7-Mediated Dendritic Cell Signaling and Migration Mechanisms in vivo.
Production and Functional Characterization of Murine Osteoclasts Differentiated from ER-Hoxb8-Immortalized Myeloid Progenitor Cells
Title: Production and Functional Characterization of Murine Osteoclasts Differentiated from ER-Hoxb8-Immortalized Myeloid Progenitor Cells.
results demonstrate an important role for the intersection of Lmx1b and Hoxb8::cre expression in the development of nociceptive dorsal horn circuits critical for mechanical and thermal pain processing.
Title: Hoxb8 intersection defines a role for Lmx1b in excitatory dorsal horn neuron development, spinofugal connectivity, and nociception.
Results show a previously unrecognised link between Hoxb8 expression and microRNAs that provides a new insight into the oncogenic functions of Hoxb8.
Title: Hoxb8 regulates expression of microRNAs to control cell death and differentiation.
Mouse Hoxb8 mutants show unexpected behavior manifested by compulsive grooming and hair removal further, transplantation of wild-type bone marrow into Hoxb8 mutant mice rescues their pathological phenotype.
Title: Hematopoietic origin of pathological grooming in Hoxb8 mutant mice.
A lower number of neurons in the upper spinal laminae and neuronal disorganization in the dorsal horn underlie the sensory defects including the excessive grooming of the Hoxb8 mutant.
Title: Loss of Hoxb8 alters spinal dorsal laminae and sensory responses in mice.
miR-196 acts upstream of Hoxb8 and Sonic hedgehog (Shh) in vivo in the context of limb development, thereby identifying a previously observed but uncharacterized inhibitory activity that operates specifically in the hindlimb
Title: The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb development.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (9) 1 citation
Endonuclease-mediated (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Hoxb (e-PCR), detects polymorphism
- UniSTS:143378

Hoxb8 (e-PCR), detects polymorphism
- UniSTS:536655

D11Mit54 (e-PCR), detects polymorphism
- UniSTS:126601

NoName (e-PCR)
- UniSTS:480723

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within adult locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within anterior/posterior pattern specification	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within anterior/posterior pattern specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within dorsal spinal cord development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic skeletal system morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within embryonic skeletal system morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within grooming behavior	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of myeloid cell differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within sensory perception of pain	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within skeletal system morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within skeletal system morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: homeobox protein Hox-B8

Names: homeo box B8; homeobox protein Hox-2.4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010461.2 → NP_034591.1 homeobox protein Hox-B8

See identical proteins and their annotated locations for NP_034591.1

Status: VALIDATED

Source sequence(s)

AK083980, AK131612, AL645478

Consensus CDS

CCDS25293.1

UniProtKB/Swiss-Prot

P09632

UniProtKB/TrEMBL

A2A9Z8

Related

ENSMUSP00000052496.3, ENSMUST00000052650.4

Conserved Domains (1) summary

pfam00046
Location:150 → 203

Homeobox; Homeobox domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

96170227..96176151

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017314295.2 → XP_017169784.1 homeobox protein Hox-B8 isoform X1

UniProtKB/Swiss-Prot

P09632

UniProtKB/TrEMBL

A2A9Z8

Conserved Domains (1) summary

pfam00046
Location:150 → 203

Homeobox; Homeobox domain
XM_011248758.3 → XP_011247060.1 homeobox protein Hox-B8 isoform X2

See identical proteins and their annotated locations for XP_011247060.1

Conserved Domains (1) summary

pfam00046
Location:149 → 202

Homeobox; Homeobox domain
XM_017314296.2 → XP_017169785.1 homeobox protein Hox-B8 isoform X1

UniProtKB/Swiss-Prot

P09632

UniProtKB/TrEMBL

A2A9Z8

Conserved Domains (1) summary

pfam00046
Location:150 → 203

Homeobox; Homeobox domain
XM_017314297.2 → XP_017169786.1 homeobox protein Hox-B8 isoform X1

UniProtKB/Swiss-Prot

P09632

UniProtKB/TrEMBL

A2A9Z8

Conserved Domains (1) summary

pfam00046
Location:150 → 203

Homeobox; Homeobox domain