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    Gapdhs glyceraldehyde-3-phosphate dehydrogenase, spermatogenic [ Mus musculus (house mouse) ]

    Gene ID: 14447, updated on 5-Mar-2024

    Summary

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    Official Symbol
    Gapdhsprovided by MGI
    Official Full Name
    glyceraldehyde-3-phosphate dehydrogenase, spermatogenicprovided by MGI
    Primary source
    MGI:MGI:95653
    See related
    Ensembl:ENSMUSG00000061099 AllianceGenome:MGI:95653
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Gapds; Gapd-s
    Summary
    Enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity. Involved in flagellated sperm motility. Acts upstream of or within glycolytic process. Located in sperm fibrous sheath and sperm principal piece. Human ortholog(s) of this gene implicated in Alzheimer's disease. Orthologous to human GAPDHS (glyceraldehyde-3-phosphate dehydrogenase, spermatogenic). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Restricted expression toward testis adult (RPKM 682.7) See more
    Orthologs
    human all
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    Genomic context

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    Location:
    7 B1; 7 19.23 cM
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 7 NC_000073.7 (30429204..30443106, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 7 NC_000073.6 (30729779..30744791, complement)

    Chromosome 7 - NC_000073.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E9108 Neighboring gene ATPase, H+/K+ exchanging, gastric, alpha polypeptide Neighboring gene STARR-positive B cell enhancer ABC_E11349 Neighboring gene transmembrane protein 147 Neighboring gene suprabasin Neighboring gene dermokine Neighboring gene keratinocyte differentiation associated protein

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Genomic organization of a mouse glyceraldehyde 3-phosphate dehydrogenase gene (Gapd-s) expressed in post-meiotic spermatogenic cells. Welch JE, et al. Dev Genet, 1995. PMID 7736666
    2. Sperm function, protein phosphorylation, and metabolism differ in mice lacking successive sperm-specific glycolytic enzymes. Huang Z, et al. Biol Reprod, 2017 Oct 1. PMID 29025010
    3. Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility. Miki K, et al. Proc Natl Acad Sci U S A, 2004 Nov 23. PMID 15546993, Free PMC Article
    4. Mouse testis brain RNA-binding protein/translin selectively binds to the messenger RNA of the fibrous sheath protein glyceraldehyde 3-phosphate dehydrogenase-S and suppresses its translation in vitro. Yang J, et al. Biol Reprod, 2003 Mar. PMID 12604635
    5. Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution during mouse spermatogenesis. Bunch DO, et al. Biol Reprod, 1998 Mar. PMID 9510974

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. These results identify multiple signaling and metabolic defects that are likely contributors to male infertility and the absence of progressive sperm motility seen in mice lacking GAPDHS.
      Title: Sperm function, protein phosphorylation, and metabolism differ in mice lacking successive sperm-specific glycolytic enzymes.
    2. Gapds(-/-) males were infertile and had profound defects in sperm motility, exhibiting sluggish movement without forward progression.
      Title: Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility.
    3. TB-RBP is involved in the posttranscriptional regulation of Gapds gene expression during spermiogenesis.
      Title: Mouse testis brain RNA-binding protein/translin selectively binds to the messenger RNA of the fibrous sheath protein glyceraldehyde 3-phosphate dehydrogenase-S and suppresses its translation in vitro.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables NAD binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables NADP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in flagellated sperm motility IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within flagellated sperm motility IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in glucose metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in glycolytic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within glycolytic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of glycolytic process TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cilium IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in motile cilium IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in motile cilium ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in sperm fibrous sheath IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in sperm principal piece IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    glyceraldehyde-3-phosphate dehydrogenase, testis-specific
    Names
    GAPDH-2
    spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
    spermatogenic glyceraldehyde-3-phosphate dehydrogenase
    NP_001277560.1
    NP_032111.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001290631.1 → NP_001277560.1  glyceraldehyde-3-phosphate dehydrogenase, testis-specific isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longer isoform (1).
      Source sequence(s)
      AC167978
      Consensus CDS
      CCDS71933.1
      UniProtKB/Swiss-Prot
      Q60650, Q64467
      UniProtKB/TrEMBL
      S4R2G5
      Related
      ENSMUSP00000138634.2, ENSMUST00000182634.8
      Conserved Domains (3) summary
      COG0057
      Location:110 → 440
      GapA; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase [Carbohydrate transport and metabolism]
      pfam00044
      Location:110 → 256
      Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
      pfam02800
      Location:261 → 418
      Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain

    2. NM_008085.2 → NP_032111.1  glyceraldehyde-3-phosphate dehydrogenase, testis-specific isoform 2

      See identical proteins and their annotated locations for NP_032111.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) contains an alternate first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.
      Source sequence(s)
      AC167978
      Consensus CDS
      CCDS21109.1
      UniProtKB/Swiss-Prot
      Q60650, Q64467
      UniProtKB/TrEMBL
      A0A0R4J0X7
      Related
      ENSMUSP00000074317.5, ENSMUST00000074758.6
      Conserved Domains (3) summary
      COG0057
      Location:108 → 438
      GapA; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase [Carbohydrate transport and metabolism]
      pfam00044
      Location:108 → 254
      Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
      pfam02800
      Location:259 → 416
      Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000073.7 Reference GRCm39 C57BL/6J

      Range
      30429204..30443106 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q64467.1 GenPept UniProtKB/Swiss-Prot:Q64467

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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