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    Sh3pxd2a SH3 and PX domains 2A [ Mus musculus (house mouse) ]

    Gene ID: 14218, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Sh3pxd2aprovided by MGI
    Official Full Name
    SH3 and PX domains 2Aprovided by MGI
    Primary source
    MGI:MGI:1298393
    See related
    Ensembl:ENSMUSG00000053617 AllianceGenome:MGI:1298393
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Fish; TSK5; Tks5; Gm5098; Sh3md1; EG329070; C230050L11; 2310014D11Rik
    Summary
    Enables phosphatidylinositol phosphate binding activity; phosphatidylinositol-3,4-bisphosphate binding activity; and protease binding activity. Involved in reactive oxygen species metabolic process. Acts upstream of or within in utero embryonic development. Located in cytoplasm and podosome. Is expressed in several structures, including brain; clavicle; liver; skeletal muscle; and spleen. Orthologous to human SH3PXD2A (SH3 and PX domains 2A). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in subcutaneous fat pad adult (RPKM 33.7), mammary gland adult (RPKM 26.4) and 24 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    19 C3; 19 39.45 cM
    Exon count:
    16
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (47248613..47453053, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (47260174..47464612, complement)

    Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene H1.11 linker histone, pseudogene Neighboring gene STARR-seq mESC enhancer starr_46341 Neighboring gene neuralized E3 ubiquitin protein ligase 1A Neighboring gene predicted gene, 36693 Neighboring gene STARR-seq mESC enhancer starr_46347 Neighboring gene microRNA 6995 Neighboring gene VISTA enhancer mm1098 Neighboring gene predicted gene, 29834 Neighboring gene STARR-positive B cell enhancer ABC_E8755 Neighboring gene STARR-seq mESC enhancer starr_46356 Neighboring gene CapStarr-seq enhancer MGSCv37_chr19:47487651-47487834 Neighboring gene STARR-positive B cell enhancer ABC_E7669 Neighboring gene STARR-positive B cell enhancer ABC_E4289 Neighboring gene CapStarr-seq enhancer MGSCv37_chr19:47523086-47523301 Neighboring gene STARR-seq mESC enhancer starr_46360 Neighboring gene predicted gene, 36885 Neighboring gene predicted gene, 52384 Neighboring gene STARR-seq mESC enhancer starr_46363 Neighboring gene predicted gene, 41858 Neighboring gene predicted gene, 19557 Neighboring gene STN1, CST complex subunit

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Tks5 activation in mesothelial cells creates invasion front of peritoneal carcinomatosis. Satoyoshi R, et al. Oncogene, 2015 Jun 11. PMID 25088196
    2. Cell biology: watching the first steps of podosome formation. Symons M. Curr Biol, 2008 Oct 14. PMID 18957242
    3. Tks5 Regulates Synaptic Podosome Formation and Stabilization of the Postsynaptic Machinery at the Neuromuscular Junction. Pęziński M, et al. Int J Mol Sci, 2021 Nov 7. PMID 34769479, Free PMC Article
    4. Orthodontic compressive force modulates Ets-1/Tks5 pathway to promote the formation of circumferential invadopodia and the fusion of osteoclast precursors. Wang Y, et al. J Cell Physiol, 2019 Aug. PMID 30523634
    5. Expression of the adaptor protein Tks5 in human cancer: prognostic potential. Stylli SS, et al. Oncol Rep, 2014 Sep. PMID 24993883

    See all (40) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. SRC and TKS5 mediated podosome formation in fibroblasts promotes extracellular matrix invasion and pulmonary fibrosis.
      Title: SRC and TKS5 mediated podosome formation in fibroblasts promotes extracellular matrix invasion and pulmonary fibrosis.
    2. Tks5 Regulates Synaptic Podosome Formation and Stabilization of the Postsynaptic Machinery at the Neuromuscular Junction.
      Title: Tks5 Regulates Synaptic Podosome Formation and Stabilization of the Postsynaptic Machinery at the Neuromuscular Junction.
    3. Compressive force upregulated the expression of Ets-1 and Tks5.
      Title: Orthodontic compressive force modulates Ets-1/Tks5 pathway to promote the formation of circumferential invadopodia and the fusion of osteoclast precursors.
    4. Suggest the importance of Tks adaptor proteins in melanoma growth and metastasis in vivo is likely via functional invadopodia formation.
      Title: The role of Tks adaptor proteins in invadopodia formation, growth and metastasis of melanoma.
    5. This is the first study that identifies a new Rab40b-Tks5- and miR-204-dependent invadopodia transport pathway that regulates MMP2 and MMP9 secretion, and extracellular matrix remodeling during cancer progression.
      Title: Rab40b and Tks5 interact at invadopodia during metastasis.
    6. Results show that TKS5 is highly expressed in many cancer types with strong correlation with increased metastatic events and a poorer prognosis suggesting a clinical importance.
      Title: Expression of the adaptor protein Tks5 in human cancer: prognostic potential.
    7. Signaling leading to Tks5 activation in peritoneal mesothelial cells may be a suitable therapeutic target for prevention of peritoneal carcinomatosis.
      Title: Tks5 activation in mesothelial cells creates invasion front of peritoneal carcinomatosis.
    8. Tks5 undergoes an isoform switch during metastatic progression in a genetically engineered mouse model of lung adenocarcinoma
      Title: Differential Tks5 isoform expression contributes to metastatic invasion of lung adenocarcinoma.
    9. Tks5, a master regulator of invadopodia in cancer cells, is crucial for osteoclast fusion downstream of phosphoinositide 3-kinase and Src.
      Title: Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell-cell fusion.
    10. Src-Tks5 pathway is required for neural crest cell migration during embryonic development
      Title: A Src-Tks5 pathway is required for neural crest cell migration during embryonic development.
    Submit: New GeneRIF Correction See all GeneRIFs (14)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • MGC144076, MGC190848, MGC190948

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables phosphatidylinositol binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables phosphatidylinositol-3,4-bisphosphate binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables phosphatidylinositol-3-phosphate binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables phosphatidylinositol-4,5-bisphosphate binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables phosphatidylinositol-4-phosphate binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables phosphatidylinositol-5-phosphate binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protease binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables superoxide-generating NADPH oxidase activator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within in utero embryonic development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in osteoclast fusion ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in reactive oxygen species metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in superoxide anion generation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in superoxide metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in anchoring junction IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cell projection IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in podosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in podosome ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    SH3 and PX domain-containing protein 2A
    Names
    SH3 multiple domains 1
    SH3 multiple domains protein 1
    five SH3 domain-containing protein
    five SH3 domains
    tyrosine kinase substrate with five SH3 domains

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001164717.2 → NP_001158189.1  SH3 and PX domain-containing protein 2A isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.
      Source sequence(s)
      AC132268, AC132288
      Consensus CDS
      CCDS50464.1
      UniProtKB/TrEMBL
      A0A494BBI9
      Related
      ENSMUSP00000107430.3, ENSMUST00000111800.4
      Conserved Domains (6) summary
      cd06888
      Location:6 → 124
      PX_FISH; The phosphoinositide binding Phox Homology domain of Five SH protein
      cd12019
      Location:810 → 860
      SH3_Tks5_4; Fourth Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12020
      Location:1039 → 1095
      SH3_Tks5_5; Fifth (C-terminal) Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12074
      Location:170 → 222
      SH3_Tks5_1; First Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12077
      Location:241 → 294
      SH3_Tks5_2; Second Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12079
      Location:422 → 475
      SH3_Tks5_3; Third Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

    2. NM_008018.5 → NP_032044.2  SH3 and PX domain-containing protein 2A isoform 1

      See identical proteins and their annotated locations for NP_032044.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AC132268, AC132288
      Consensus CDS
      CCDS29891.1
      UniProtKB/Swiss-Prot
      E9QKJ2, O89032, Q148Q8
      UniProtKB/TrEMBL
      A0A494BBI9
      Related
      ENSMUSP00000080325.3, ENSMUST00000081619.10
      Conserved Domains (6) summary
      cd06888
      Location:6 → 124
      PX_FISH; The phosphoinositide binding Phox Homology domain of Five SH protein
      cd12019
      Location:838 → 888
      SH3_Tks5_4; Fourth Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12020
      Location:1067 → 1123
      SH3_Tks5_5; Fifth (C-terminal) Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12074
      Location:170 → 222
      SH3_Tks5_1; First Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12077
      Location:269 → 322
      SH3_Tks5_2; Second Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12079
      Location:450 → 503
      SH3_Tks5_3; Third Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000085.7 Reference GRCm39 C57BL/6J

      Range
      47248613..47453053 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036161432.1 → XP_036017325.1  SH3 and PX domain-containing protein 2A isoform X2

      UniProtKB/TrEMBL
      A0A494BBI9
      Conserved Domains (6) summary
      cd06888
      Location:6 → 124
      PX_FISH; The phosphoinositide binding Phox Homology domain of Five SH protein
      cd12019
      Location:795 → 845
      SH3_Tks5_4; Fourth Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12020
      Location:1024 → 1080
      SH3_Tks5_5; Fifth (C-terminal) Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12074
      Location:155 → 207
      SH3_Tks5_1; First Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12077
      Location:226 → 279
      SH3_Tks5_2; Second Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12079
      Location:407 → 460
      SH3_Tks5_3; Third Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

    2. XM_006526669.5 → XP_006526732.1  SH3 and PX domain-containing protein 2A isoform X1

      UniProtKB/TrEMBL
      A0A494BBI9
      Conserved Domains (6) summary
      cd06888
      Location:6 → 124
      PX_FISH; The phosphoinositide binding Phox Homology domain of Five SH protein
      cd12019
      Location:823 → 873
      SH3_Tks5_4; Fourth Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12020
      Location:1052 → 1108
      SH3_Tks5_5; Fifth (C-terminal) Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12074
      Location:155 → 207
      SH3_Tks5_1; First Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12077
      Location:254 → 307
      SH3_Tks5_2; Second Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12079
      Location:435 → 488
      SH3_Tks5_3; Third Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains

    3. XM_006526670.5 → XP_006526733.1  SH3 and PX domain-containing protein 2A isoform X3

      Conserved Domains (3) summary
      cd12019
      Location:368 → 418
      SH3_Tks5_4; Fourth Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cd12020
      Location:597 → 653
      SH3_Tks5_5; Fifth (C-terminal) Src homology 3 domain of Tyrosine kinase substrate with five SH3 domains
      cl17036
      Location:4 → 33
      SH3; Src Homology 3 domain superfamily

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_177833.2: Suppressed sequence

      Description
      NM_177833.2: This RefSeq was permanently suppressed because currently there is support for the transcript but not for the protein.
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O89032.2 GenPept UniProtKB/Swiss-Prot:O89032

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