Ddost dolichyl-di-phosphooligosaccharide-protein glycotransferase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ddostprovided by MGI
Official Full Name: dolichyl-di-phosphooligosaccharide-protein glycotransferaseprovided by MGI
Primary source: MGI:MGI:1194508
See related: Ensembl:ENSMUSG00000028757 AllianceGenome:MGI:1194508
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Predicted to enable enzyme activator activity. Predicted to be involved in several processes, including T cell activation; protein N-linked glycosylation via asparagine; and regulation of protein stability. Predicted to be located in endoplasmic reticulum. Predicted to be part of oligosaccharyltransferase complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation Ir. Orthologous to human DDOST (dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in colon adult (RPKM 167.1), ovary adult (RPKM 166.7) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 D3; 4 70.13 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (138032049..138039930)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (138304738..138312619)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR-532-3p/DDOST axis.
Title: LncRNA TSIX aggravates spinal cord injury by regulating the PI3K/AKT pathway via the miR-532-3p/DDOST axis.
Globally elevating the AGE clearance receptor, OST48, does not protect against the development of diabetic kidney disease, despite improving insulin secretion.
Title: Globally elevating the AGE clearance receptor, OST48, does not protect against the development of diabetic kidney disease, despite improving insulin secretion.
Study demonstrated that dolichyl-diphosphooligosaccharide-protein glycosyltransferase Ost48 (encoded by Ddost gene) is a facilitator of increased advanced glycation end-products (AGE) deposition in the liver and OST48-AGE pathway leads to the onset of liver injury in combination with central adiposity and glucose intolerance, despite ample physical activity.
Title: Increased liver AGEs induce hepatic injury mediated through an OST48 pathway.
the cell-cycle-dependent post-translation modification of TREX1 regulates its interaction with OST.
Title: Mitotic Phosphorylation of TREX1 C Terminus Disrupts TREX1 Regulation of the Oligosaccharyltransferase Complex.
Data show that oligosaccharyltransferase (OST) activity is dysregulated in three prime exonuclease 1 knockout (Trex1-/-) cells.
Title: Cytosolic Nuclease TREX1 Regulates Oligosaccharyltransferase Activity Independent of Nuclease Activity to Suppress Immune Activation.
AGER1 provides protection against AGE-induced reactive oxygen species generation via NADPH oxidase and protein kinase C delta.
Title: AGER1 regulates endothelial cell NADPH oxidase-dependent oxidant stress via PKC-delta: implications for vascular disease.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:488240

RH130187 (e-PCR)
- UniSTS:213474

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables enzyme activator activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in T cell activation	ISO Inferred from Sequence Orthology more info
involved_in protein N-linked glycosylation	ISO Inferred from Sequence Orthology more info
involved_in protein N-linked glycosylation via asparagine	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein N-linked glycosylation via asparagine	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein stability	ISO Inferred from Sequence Orthology more info
involved_in response to cytokine	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum membrane	NAS Non-traceable Author Statement more info	PubMed
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
part_of oligosaccharyltransferase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of oligosaccharyltransferase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Names: DDOST 48 kDa subunit; oligosaccharyl transferase 48 kDa subunit

NP_031864.2

EC 2.4.99.18

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_007838.3 → NP_031864.2 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor

See identical proteins and their annotated locations for NP_031864.2

Status: VALIDATED

Source sequence(s)

BC068132, BU755176

Consensus CDS

CCDS18824.1

UniProtKB/Swiss-Prot

O54734, Q8C4P7

UniProtKB/TrEMBL

Q3UC51

Related

ENSMUSP00000030538.5, ENSMUST00000030538.5

Conserved Domains (1) summary

pfam03345
Location:33 → 440

DDOST_48kD; Oligosaccharyltransferase 48 kDa subunit beta