Crk v-crk avian sarcoma virus CT10 oncogene homolog [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Crkprovided by MGI
Official Full Name: v-crk avian sarcoma virus CT10 oncogene homologprovided by MGI
Primary source: MGI:MGI:88508
See related: Ensembl:ENSMUSG00000017776 AllianceGenome:MGI:88508
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: p38; Crk3; Crko; Crk-I; c-Crk; Crk-II; CrkIII; Crk-III
Summary: This gene is part of a family of adapter proteins that mediate formation of signal transduction complexes in response to extracellular stimuli, such as growth and differentiation factors. Protein-protein interactions occur through the SH2 domain, which binds phosphorylated tyrosine residues, and the SH3 domain, which binds proline-rich peptide motifs. These interactions promote recruitment and activation of effector proteins to regulate cell migration, adhesion, and proliferation. In mouse this protein is essential for embryonic development. Alternatively spliced transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Mar 2013]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 18.5), bladder adult (RPKM 18.1) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B5; 11 45.92 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (75570020..75599254)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (75679194..75708428)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Crk and Crkl Are Required in the Endocardial Lineage for Heart Valve Development.
Title: Crk and Crkl Are Required in the Endocardial Lineage for Heart Valve Development.
Cell shape-based chemical screening reveals an epigenetic network mediated by focal adhesions.
Title: Cell shape-based chemical screening reveals an epigenetic network mediated by focal adhesions.
Essential role of the Crk family-dosage in DiGeorge-like anomaly and metabolic homeostasis.
Title: Essential role of the Crk family-dosage in DiGeorge-like anomaly and metabolic homeostasis.
Requirement for Crk and CrkL during postnatal lens development.
Title: Requirement for Crk and CrkL during postnatal lens development.
Signaling adaptor protein Crk is involved in malignant feature of pancreatic cancer associated with phosphorylation of c-Met.
Title: Signaling adaptor protein Crk is involved in malignant feature of pancreatic cancer associated with phosphorylation of c-Met.
these results indicate that CrkII negatively regulates osteoblast differentiation and function through JNK phosphorylation. Given that CrkII acts as a negative and positive regulator of osteoblast and osteoclast differentiation, respectively, the regulation of CrkII expression in bone cells may help to develop new strategies to enhance bone formation and inhibit bone resorption.
Title: Adaptor protein CrkII negatively regulates osteoblast differentiation and function through JNK phosphorylation.
Here, we show that the Crk family of adaptor proteins, Crk and Crkl, are required for mouse lens morphogenesis but not differentiation upon Fibroblast Growth Factor stimulation.
Title: Crk proteins transduce FGF signaling to promote lens fiber cell elongation.
The role of Crk family proteins in NK cell function and host defense.
Title: Crk Adaptor Proteins Regulate NK Cell Expansion and Differentiation during Mouse Cytomegalovirus Infection.
Findings delineate a novel role for Crk Tyr239 phosphorylation in the regulation of Src kinases, as well as a potential molecular explanation for a long-standing question as to how Crk regulates the activation of Src kinases.
Title: Crk Tyrosine Phosphorylation Regulates PDGF-BB-inducible Src Activation and Breast Tumorigenicity and Metastasis.
These results indicate that CCRK functions in eye development by both positively and negatively regulating the Hh pathway, and they reveal distinct requirements for Hh signaling in patterning and morphogenesis of the eyes.
Title: Cell cycle-related kinase regulates mammalian eye development through positive and negative regulation of the Hedgehog pathway.

Submit: New GeneRIF Correction See all GeneRIFs (36)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Gene trapped (1) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Crk (e-PCR), detects polymorphism
- UniSTS:265807

Crk (e-PCR), detects polymorphism
- UniSTS:265808

CRK (e-PCR)
- UniSTS:266693

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SH2 domain binding	ISO Inferred from Sequence Orthology more info
enables SH3 domain binding	ISO Inferred from Sequence Orthology more info
enables cytoskeletal protein binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables ephrin receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables ephrin receptor binding	ISO Inferred from Sequence Orthology more info
enables insulin-like growth factor receptor binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables phosphotyrosine residue binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein phosphorylated amino acid binding	IDA Inferred from Direct Assay more info	PubMed
enables protein phosphorylated amino acid binding	ISO Inferred from Sequence Orthology more info
enables protein tyrosine kinase binding	ISO Inferred from Sequence Orthology more info
enables protein-macromolecule adaptor activity	ISO Inferred from Sequence Orthology more info
enables receptor tyrosine kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables scaffold protein binding	ISO Inferred from Sequence Orthology more info
enables signaling adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables signaling adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables signaling adaptor activity	ISO Inferred from Sequence Orthology more info
enables signaling receptor complex adaptor activity	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within activation of GTPase activity	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cell chemotaxis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to endothelin	ISO Inferred from Sequence Orthology more info
involved_in cellular response to insulin-like growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to nerve growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to nitric oxide	ISO Inferred from Sequence Orthology more info
involved_in cellular response to transforming growth factor beta stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cerebellar neuron development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cerebral cortex development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within dendrite development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in enzyme-linked receptor protein signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in enzyme-linked receptor protein signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in enzyme-linked receptor protein signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in enzyme-linked receptor protein signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ephrin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within helper T cell diapedesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within hippocampus development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cell motility	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of natural killer cell mediated cytotoxicity	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of wound healing	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuron migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of JNK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of Rac protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of skeletal muscle acetylcholine-gated channel clustering	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of skeletal muscle acetylcholine-gated channel clustering	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of skeletal muscle acetylcholine-gated channel clustering	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of smooth muscle cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of substrate adhesion-dependent cell spreading	ISO Inferred from Sequence Orthology more info
involved_in postsynaptic specialization assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in postsynaptic specialization assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to membrane	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within reelin-mediated signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of GTPase activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of Rac protein signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of T cell migration	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of actin cytoskeleton organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cell adhesion mediated by integrin	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell shape	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of dendrite development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of intracellular signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of leukocyte migration	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of protein binding	ISO Inferred from Sequence Orthology more info
involved_in regulation of signal transduction	ISO Inferred from Sequence Orthology more info
involved_in response to cholecystokinin	ISO Inferred from Sequence Orthology more info
involved_in response to hepatocyte growth factor	ISO Inferred from Sequence Orthology more info
involved_in response to hydrogen peroxide	ISO Inferred from Sequence Orthology more info
involved_in response to peptide	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in neuromuscular junction	IMP Inferred from Mutant Phenotype more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: adapter molecule crk

Names: CT-10 related kinase 3; avian sarcoma virus CT10 (v-crk) oncogene homolog; proto-oncogene c-crk; v-crk sarcoma virus CT10 oncogene-like protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001277219.1 → NP_001264148.1 adapter molecule crk isoform 1

See identical proteins and their annotated locations for NP_001264148.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the shorter isoform 1 (also known as CrkI). Isoform 1 (CrkI) contains the SH2 domain and the N-terminal SH3 domain, both of which are proposed to be crucial for development.

Source sequence(s)

AK139089, AL591440

Consensus CDS

CCDS70241.1

UniProtKB/TrEMBL

Q8JZR2

Related

ENSMUSP00000104063.2, ENSMUST00000108425.8

Conserved Domains (2) summary

cd09926
Location:5 → 122

SH2_CRK_like; Src homology 2 domain found in cancer-related signaling adaptor protein CRK

cd11758
Location:135 → 189

SH3_CRK_N; N-terminal Src Homology 3 domain of Ct10 Regulator of Kinase adaptor proteins
NM_001277221.1 → NP_001264150.1 adapter molecule crk isoform 3

See identical proteins and their annotated locations for NP_001264150.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks an alternate, in-frame exon in the coding region compared to variant 1. The encoded isoform (3) is shorter than isoform 1 (CrkI). Isoform 3 does not have a complete SH2 domain and lacks an SH3 domain, both of which are found in isoform 1.

Source sequence(s)

AL591440

Consensus CDS

CCDS70242.1

UniProtKB/TrEMBL

Q3TQV3

Related

ENSMUSP00000104064.2, ENSMUST00000108426.8

Conserved Domains (1) summary

cl15255
Location:5 → 63

SH2; Src homology 2 (SH2) domain
NM_133656.5 → NP_598417.2 adapter molecule crk isoform 2

See identical proteins and their annotated locations for NP_598417.2

Status: REVIEWED

Description

Transcript Variant: This variant (2) includes an alternate segment, compared to variant 1, resulting in a longer protein (isoform 2, also known as CrkII) that has a distinct C-terminus and an additional SH3 domain, compared to isoform 1 (CrkI). Isoform 2 (CrkII) is proposed to have a negative regulatory role.

Source sequence(s)

AK028488, AL591440

Consensus CDS

CCDS25055.1

UniProtKB/Swiss-Prot

Q64010

UniProtKB/TrEMBL

Q5ND51, Q91VM1

Related

ENSMUSP00000017920.8, ENSMUST00000017920.14

Conserved Domains (3) summary

cd09926
Location:5 → 122

SH2_CRK_like; Src homology 2 domain found in cancer-related signaling adaptor protein CRK

cd11758
Location:135 → 189

SH3_CRK_N; N-terminal Src Homology 3 domain of Ct10 Regulator of Kinase adaptor proteins

cd11759
Location:237 → 293

SH3_CRK_C; C-terminal Src Homology 3 domain of Ct10 Regulator of Kinase adaptor proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

75570020..75599254

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006532125.3 → XP_006532188.1 adapter molecule crk isoform X2

See identical proteins and their annotated locations for XP_006532188.1

UniProtKB/TrEMBL

Q3TTI0, Q5ND50

Related

ENSMUSP00000090803.4, ENSMUST00000093115.4

Conserved Domains (2) summary

cd09926
Location:5 → 122

SH2_CRK_like; Src homology 2 domain found in cancer-related signaling adaptor protein CRK

cd11758
Location:135 → 189

SH3_CRK_N; N-terminal Src Homology 3 domain of Ct10 Regulator of Kinase adaptor proteins
XM_006532124.4 → XP_006532187.1 adapter molecule crk isoform X1

See identical proteins and their annotated locations for XP_006532187.1

UniProtKB/TrEMBL

Q920I1

Related

ENSMUSP00000116527.2, ENSMUST00000147718.2

Conserved Domains (3) summary

cd09926
Location:5 → 122

SH2_CRK_like; Src homology 2 domain found in cancer-related signaling adaptor protein CRK

cd11758
Location:135 → 189

SH3_CRK_N; N-terminal Src Homology 3 domain of Ct10 Regulator of Kinase adaptor proteins

cl17036
Location:237 → 259

SH3; Src Homology 3 domain superfamily