Cpa3 carboxypeptidase A3, mast cell [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cpa3provided by MGI
Official Full Name: carboxypeptidase A3, mast cellprovided by MGI
Primary source: MGI:MGI:88479
See related: Ensembl:ENSMUSG00000001865 AllianceGenome:MGI:88479
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MC-CPA
Summary: This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases and preproprotein that is proteolytically processed to generate a mature protein product. This product is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Homozygous knockout mice for this gene exhibit impaired mast cell development. [provided by RefSeq, Aug 2015]
Expression: Biased expression in limb E14.5 (RPKM 23.7), mammary gland adult (RPKM 5.5) and 4 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 A2; 3 6.25 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (20269780..20296373, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (20215616..20242209, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Disruption of the mast cell carboxypeptidase A3 gene does not attenuate airway inflammation and hyperresponsiveness in two mouse models of asthma.
Title: Disruption of the mast cell carboxypeptidase A3 gene does not attenuate airway inflammation and hyperresponsiveness in two mouse models of asthma.
The goal of the current study was to compare bone repair in normal wild type and Cpa3Cre/+ mice, which lack mast cells in the absence of any other hematopoietic or non- hematopoietic deficiencies. Mast cells appear in healing bone of wild type mice but not Cpa3Cre/+ mice, beginning 14 days after surgery. Re-vascularization of repair tissue and deposition of mineralized bone was delayed and dis-organised in Cpa3Cre/+ mice
Title: Defective bone repair in mast cell-deficient Cpa3Cre/+ mice.
Mast cells in experimental arthritis contained high levels of mMC-CPA mRNA, and also lacked the exopeptidase.
Title: Experimental Arthritis Is Dependent on Mouse Mast Cell Protease-5.
Data suggest that carboxypeptidase A3 (Cpa3)-Cre; myeloid cell leukemia sequence 1 (Mcl-1)(fl/fl) mice may be useful in analyzing the roles of mast cells and basophils in health and disease.
Title: Reduced mast cell and basophil numbers and function in Cpa3-Cre; Mcl-1fl/fl mice.
Mc-cpa plays a crucial role for the generation of phenotypically mature mast cells.
Title: Loss of histochemical identity in mast cells lacking carboxypeptidase A.
cathepsin E is located in mast-cell secretory granules in complex with heparin proteoglycans, and has a role in the processing of procarboxypeptidase A into active protease
Title: A role for cathepsin E in the processing of mast-cell carboxypeptidase A.
mast cell chymase and CPA have key roles in both the generation and degradation of Ang II
Title: Cooperation between mast cell carboxypeptidase A and the chymase mouse mast cell protease 4 in the formation and degradation of angiotensin II.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Endonuclease-mediated (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

J05118 (e-PCR)
- UniSTS:159098

J05118 (e-PCR)
- UniSTS:124857

Cpa3 (e-PCR), detects polymorphism
- UniSTS:527292

Cpa3 (e-PCR), detects polymorphism
- UniSTS:527041

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables carboxypeptidase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metallocarboxypeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metallocarboxypeptidase activity	ISO Inferred from Sequence Orthology more info
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of angiotensin levels in blood	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: mast cell carboxypeptidase A

NP_031779.1

EC 3.4.17.1

XP_006535445.1

EC 3.4.17.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_007753.2 → NP_031779.1 mast cell carboxypeptidase A preproprotein

See identical proteins and their annotated locations for NP_031779.1

Status: REVIEWED

Source sequence(s)

BY113342, BY584193, J05118

Consensus CDS

CCDS17262.1

UniProtKB/Swiss-Prot

P15089

UniProtKB/TrEMBL

Q542E3

Related

ENSMUSP00000001921.2, ENSMUST00000001921.3

Conserved Domains (2) summary

pfam02244
Location:28 → 102

Propep_M14; Carboxypeptidase activation peptide

cl11393
Location:114 → 413

Peptidase_M14_like; M14 family of metallocarboxypeptidases and related proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

20269780..20296373 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006535382.4 → XP_006535445.1 mast cell carboxypeptidase A isoform X1

Conserved Domains (2) summary

pfam02244
Location:32 → 103

Propep_M14; Carboxypeptidase activation peptide

cl11393
Location:114 → 330

Peptidase_M14_like; M14 family of metallocarboxypeptidases and related proteins