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    Chka choline kinase alpha [ Mus musculus (house mouse) ]

    Gene ID: 12660, updated on 21-Apr-2024

    Summary

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    Official Symbol
    Chkaprovided by MGI
    Official Full Name
    choline kinase alphaprovided by MGI
    Primary source
    MGI:MGI:107760
    See related
    Ensembl:ENSMUSG00000024843 AllianceGenome:MGI:107760
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    CK; EK; Chk; ChoK; EtnK-alpha; CK/EK-alpha; Chetk-alpha
    Summary
    Enables choline kinase activity; cholinesterase activity; and identical protein binding activity. Acts upstream of or within phosphatidylcholine biosynthetic process. Predicted to be active in cytoplasm. Is expressed in intestine; metanephros; neural retina; and telencephalon. Orthologous to human CHKA (choline kinase alpha). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in duodenum adult (RPKM 12.5), small intestine adult (RPKM 12.2) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    19 A; 19 3.57 cM
    Exon count:
    16
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (3901585..3944368)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (3851585..3894369)

    Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor pseudogene Neighboring gene STARR-seq mESC enhancer starr_45331 Neighboring gene STARR-positive B cell enhancer mm9_chr19:3851584-3851885 Neighboring gene microRNA 12192 Neighboring gene STARR-seq mESC enhancer starr_45333 Neighboring gene STARR-seq mESC enhancer starr_45334 Neighboring gene T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 Neighboring gene STARR-positive B cell enhancer ABC_E9987 Neighboring gene NADH:ubiquinone oxidoreductase core subunit S8

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Early embryonic lethality caused by disruption of the gene for choline kinase alpha, the first enzyme in phosphatidylcholine biosynthesis. Wu G, et al. J Biol Chem, 2008 Jan 18. PMID 18029352, Free PMC Article
    2. Phosphatidylcholine biosynthesis during neuronal differentiation and its role in cell fate determination. Marcucci H, et al. J Biol Chem, 2010 Aug 13. PMID 20525991, Free PMC Article
    3. Differential expression of choline kinase isoforms in skeletal muscle explains the phenotypic variability in the rostrocaudal muscular dystrophy mouse. Wu G, et al. Biochim Biophys Acta, 2010 Apr. PMID 20026284
    4. Induction of choline kinase alpha by carbon tetrachloride (CCl4) occurs via increased binding of c-jun to an AP-1 element. Aoyama C, et al. Biochim Biophys Acta, 2007 Sep. PMID 17728180
    5. Generation and characterization of monoclonal antibodies against choline kinase alpha and their potential use as diagnostic tools in cancer. Gallego-Ortega D, et al. Int J Oncol, 2006 Aug. PMID 16820874

    See all (36) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. hepatic phosphatidylcholine is regulated by the circadian clock through a Bmal1-Rev-erbalpha-Chkalpha axis
      Title: The hepatic circadian clock regulates the choline kinase α gene through the BMAL1-REV-ERBα axis.
    2. Enforced expression of either CK(alpha) or CCTalpha increased the rate of synthesis and the amount of PtdCho, and these cells initiated neuronal differentiation.
      Title: Phosphatidylcholine biosynthesis during neuronal differentiation and its role in cell fate determination.
    3. Lack of muscle degeneration in forelimbs of Chkb(-/-) mice is due to abundant choline kinase alpha and the stable homeostasis of phosphatidylcholine.
      Title: Differential expression of choline kinase isoforms in skeletal muscle explains the phenotypic variability in the rostrocaudal muscular dystrophy mouse.
    4. Chka is an essential gene for early embryonic development, but adult mice do not require full expression of the gene for normal levels of phosphatidylcholine.
      Title: Early embryonic lethality caused by disruption of the gene for choline kinase alpha, the first enzyme in phosphatidylcholine biosynthesis.
    5. Data suggest that the choline kinase alpha promoter has a distal element (-875/-867) that binds c-jun and the binding of c-jun is enhanced by treatment with CCl4.
      Title: Induction of choline kinase alpha by carbon tetrachloride (CCl4) occurs via increased binding of c-jun to an AP-1 element.
    6. ChoK may have a role in carcinogenesis
      Title: Generation and characterization of monoclonal antibodies against choline kinase alpha and their potential use as diagnostic tools in cancer.
    7. Full-length cDNAs or their truncated or alanine-mutated versions for mouse CK-alpha1 and CK-beta were expressed in COS-7 cells.
      Title: Deletion and alanine mutation analyses for the formation of active homo- or hetero-dimer complexes of mouse choline kinase-alpha and -beta.
    8. Experimental evidence for the presence of heteromeric ChoK/EtnK in any source. The activity of ChoK/EtnK in the cell is controlled not only by the level of each isoform but also by their combination to form the active oligomer complex.
      Title: Expression and characterization of the active molecular forms of choline/ethanolamine kinase-alpha and -beta in mouse tissues, including carbon tetrachloride-induced liver.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Gene trapped (1)  1 citation
    • Targeted (1) 
    • Endonuclease-mediated (4) 

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ATP binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables choline binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables choline kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables choline kinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables choline kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables cholinesterase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ethanolamine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ethanolamine kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables kinase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein tyrosine kinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in CDP-choline pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within CDP-choline pathway IDA
    Inferred from Direct Assay
    more info
    		  
    involved_in cellular response to glucose starvation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in choline metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in ethanolamine metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_positive_effect lipid droplet disassembly ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within lipid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within phosphatidylcholine biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in phosphatidylcholine biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in phosphatidylethanolamine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in phosphatidylethanolamine biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within phospholipid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within phosphorylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in lipid droplet ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    choline kinase alpha
    Names
    choline/ethanolamine kinase alpha
    ethanolamine kinase
    NP_001258425.1
    NP_001397062.1
    NP_038518.2
    XP_006531711.1
    XP_011246901.1
    XP_017173537.1
    XP_017173538.1
    XP_030106584.1
    XP_030106585.1
    XP_030106586.1
    XP_030106587.1
    XP_030106588.1
    XP_030106589.1
    XP_030106590.1
    XP_030106591.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001271496.2 → NP_001258425.1  choline kinase alpha isoform 3

      See identical proteins and their annotated locations for NP_001258425.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) contains an alternate in-frame exon, compared to variant 1. The encoded isoform (3) is longer than isoform 1.
      Source sequence(s)
      AC133523
      Consensus CDS
      CCDS70913.1
      UniProtKB/Swiss-Prot
      G5E853, O54804, Q8BPL3, Q8BPW6, Q9CXP3, Q9QX56
      UniProtKB/TrEMBL
      Q3TLJ9
      Related
      ENSMUSP00000025760.7, ENSMUST00000025760.13
      Conserved Domains (2) summary
      PLN02236
      Location:95 → 451
      PLN02236; choline kinase
      cd05156
      Location:107 → 448
      ChoK_euk; Euykaryotic Choline Kinase

    2. NM_001410133.1 → NP_001397062.1  choline kinase alpha isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC133523

    3. NM_013490.5 → NP_038518.2  choline kinase alpha isoform 1

      See identical proteins and their annotated locations for NP_038518.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes isoform 1.
      Source sequence(s)
      AC133523
      Consensus CDS
      CCDS29400.1
      UniProtKB/TrEMBL
      Q3TLJ9
      Related
      ENSMUSP00000071933.7, ENSMUST00000072055.13
      Conserved Domains (2) summary
      PLN02236
      Location:95 → 433
      PLN02236; choline kinase
      cd05156
      Location:107 → 430
      ChoK_euk; Euykaryotic Choline Kinase

    RNA

    1. NR_073190.2 RNA Sequence

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) contains two alternate internal exons, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AC133523
      Related
      ENSMUST00000128694.8

    2. NR_176954.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC133523

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000085.7 Reference GRCm39 C57BL/6J

      Range
      3901585..3944368
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030250724.2 → XP_030106584.1  choline kinase alpha isoform X2

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    2. XM_011248599.4 → XP_011246901.1  choline kinase alpha isoform X2

      See identical proteins and their annotated locations for XP_011246901.1

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    3. XM_030250726.2 → XP_030106586.1  choline kinase alpha isoform X2

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    4. XM_030250725.2 → XP_030106585.1  choline kinase alpha isoform X2

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    5. XM_030250728.2 → XP_030106588.1  choline kinase alpha isoform X2

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    6. XM_030250727.2 → XP_030106587.1  choline kinase alpha isoform X2

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    7. XM_030250730.2 → XP_030106590.1  choline kinase alpha isoform X2

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    8. XM_006531648.4 → XP_006531711.1  choline kinase alpha isoform X2

      See identical proteins and their annotated locations for XP_006531711.1

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    9. XM_030250729.2 → XP_030106589.1  choline kinase alpha isoform X2

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    10. XM_017318048.3 → XP_017173537.1  choline kinase alpha isoform X2

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    11. XM_030250731.2 → XP_030106591.1  choline kinase alpha isoform X2

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    12. XM_017318049.3 → XP_017173538.1  choline kinase alpha isoform X2

      UniProtKB/TrEMBL
      Q99KD4
      Conserved Domains (1) summary
      cd05156
      Location:1 → 276
      ChoK_euk; Euykaryotic Choline Kinase

    RNA

    1. XR_388248.4 RNA Sequence

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_001025566.1: Suppressed sequence

      Description
      NM_001025566.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O54804.3 GenPept UniProtKB/Swiss-Prot:O54804

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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