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    Ch25h cholesterol 25-hydroxylase [ Mus musculus (house mouse) ]

    Gene ID: 12642, updated on 11-Apr-2024

    Summary

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    Official Symbol
    Ch25hprovided by MGI
    Official Full Name
    cholesterol 25-hydroxylaseprovided by MGI
    Primary source
    MGI:MGI:1333869
    See related
    Ensembl:ENSMUSG00000050370 AllianceGenome:MGI:1333869
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    m25OH
    Summary
    Enables cholesterol 25-hydroxylase activity. Involved in B cell chemotaxis. Acts upstream of or within cholesterol metabolic process. Predicted to be located in endoplasmic reticulum. Predicted to be integral component of membrane. Predicted to be active in endoplasmic reticulum membrane. Is expressed in several structures, including genitourinary system; gut; hemolymphoid system gland; respiratory system; and skin. Orthologous to human CH25H (cholesterol 25-hydroxylase). [provided by Alliance of Genome Resources, Apr 2022]
    Orthologs
    human all
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    Genomic context

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    See Ch25h in Genome Data Viewer
    Location:
    19 C1; 19 29.69 cM
    Exon count:
    1
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (34451183..34452548, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (34473783..34475148, complement)

    Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 31355 Neighboring gene STARR-seq mESC enhancer starr_45994 Neighboring gene Fas cell surface death receptor Neighboring gene STARR-seq mESC enhancer starr_45995 Neighboring gene STARR-positive B cell enhancer mm9_chr19:34575305-34575606 Neighboring gene lysosomal acid lipase A Neighboring gene STARR-seq mESC enhancer starr_45997 Neighboring gene STARR-positive B cell enhancer ABC_E5697 Neighboring gene predicted gene, 31417

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to Streptococcus pneumoniae Infection. Cho SJ, et al. Cells, 2023 Feb 10. PMID 36831236, Free PMC Article
    2. A Proinflammatory Stimulus Disrupts Hippocampal Plasticity and Learning via Microglial Activation and 25-Hydroxycholesterol. Izumi Y, et al. J Neurosci, 2021 Dec 8. PMID 34725187, Free PMC Article
    3. Cholesterol 25-hydroxylase is a metabolic switch to constrain T cell-mediated inflammation in the skin. Takahashi H, et al. Sci Immunol, 2021 Oct 15. PMID 34623903, Free PMC Article
    4. Tumor Necrosis Factor-alpha utilizes MAPK/NFκB pathways to induce cholesterol-25 hydroxylase for amplifying pro-inflammatory response via 25-hydroxycholesterol-integrin-FAK pathway. Pokharel SM, et al. PLoS One, 2021. PMID 34551004, Free PMC Article
    5. Regulation of intercellular biomolecule transfer-driven tumor angiogenesis and responses to anticancer therapies. Lu Z, et al. J Clin Invest, 2021 May 17. PMID 33998600, Free PMC Article

    See all (63) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Cholesterol 25-hydroxylase mediates neuroinflammation and neurodegeneration in a mouse model of tauopathy.
      Title: Cholesterol 25-hydroxylase mediates neuroinflammation and neurodegeneration in a mouse model of tauopathy.
    2. Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to Streptococcus pneumoniae Infection.
      Title: Role of Cholesterol 25-Hydroxylase (Ch25h) in Mediating Innate Immune Responses to Streptococcus pneumoniae Infection.
    3. Cholesterol 25-hydroxylase is a metabolic switch to constrain T cell-mediated inflammation in the skin.
      Title: Cholesterol 25-hydroxylase is a metabolic switch to constrain T cell-mediated inflammation in the skin.
    4. Cholesterol 25-hydroxylase (CH25H) as a promoter of adipose tissue inflammation in obesity and diabetes.
      Title: Cholesterol 25-hydroxylase (CH25H) as a promoter of adipose tissue inflammation in obesity and diabetes.
    5. Cholesterol 25-hydroxylase promotes efferocytosis and resolution of lung inflammation.
      Title: Cholesterol 25-hydroxylase promotes efferocytosis and resolution of lung inflammation.
    6. Overexpression of both human and murine CH25H inhibited rabies virus (RABV) infection in HEK-293T cells.
      Title: Cholesterol 25-hydroxylase suppresses rabies virus infection by inhibiting viral entry.
    7. results indicate that osteoarthritis is a disease associated with metabolic disorders and suggest that targeting the CH25H-CYP7B1-RORalpha axis of cholesterol metabolism may provide a therapeutic avenue for treating osteoarthritis.
      Title: The CH25H-CYP7B1-RORα axis of cholesterol metabolism regulates osteoarthritis.
    8. Adenovirus-mediated Ch25h overexpression in mice improved glucose tolerance and insulin sensitivity and lowered HOMA-IR. Our data suggest that low hepatic Ch25h levels could be considered a risk marker for unhealthy obesity.
      Title: Hepatic Cholesterol-25-Hydroxylase Overexpression Improves Systemic Insulin Sensitivity in Mice.
    9. Ch25h-deficient mice exhibit increased sensitivity to septic shock, exacerbated experimental autoimmune encephalomyelitis, and a stronger ability to repress bacterial growth.
      Title: Inflammation. 25-Hydroxycholesterol suppresses interleukin-1-driven inflammation downstream of type I interferon.
    10. Disruption of Roralpha1 and cholesterol 25-hydroxylase expression attenuates phagocytosis in male Roralphasg/sg mice.
      Title: Disruption of Rorα1 and cholesterol 25-hydroxylase expression attenuates phagocytosis in male Rorαsg/sg mice.
    Submit: New GeneRIF Correction See all GeneRIFs (14)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables C-4 methylsterol oxidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables cholesterol 25-hydroxylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cholesterol 25-hydroxylase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables cholesterol 25-hydroxylase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables iron ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables monooxygenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables steroid hydroxylase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables steroid hydroxylase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in B cell chemotaxis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cholesterol metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within cholesterol metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in cholesterol metabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in lipid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within lipid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of cholesterol metabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of fusion of virus membrane with host plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in response to type I interferon ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within steroid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within steroid metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in sterol biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in endoplasmic reticulum membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    cholesterol 25-hydroxylase
    Names
    cholesterol 25-monooxygenase
    NP_034020.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_009890.2 → NP_034020.1  cholesterol 25-hydroxylase

      See identical proteins and their annotated locations for NP_034020.1

      Status: VALIDATED

      Source sequence(s)
      AC102249
      Consensus CDS
      CCDS29759.1
      UniProtKB/Swiss-Prot
      Q3TUM6, Q8CHQ2, Q9Z0F5
      Related
      ENSMUSP00000049683.5, ENSMUST00000050562.6
      Conserved Domains (2) summary
      COG3000
      Location:128 → 271
      ERG3; Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism]
      pfam04116
      Location:128 → 263
      FA_hydroxylase; Fatty acid hydroxylase superfamily

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000085.7 Reference GRCm39 C57BL/6J

      Range
      34451183..34452548 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9Z0F5.1 GenPept UniProtKB/Swiss-Prot:Q9Z0F5

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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