Car3 carbonic anhydrase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Car3provided by MGI
Official Full Name: carbonic anhydrase 3provided by MGI
Primary source: MGI:MGI:88270
See related: Ensembl:ENSMUSG00000027559 AllianceGenome:MGI:88270
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ca3; Car-3
Summary: Enables nickel cation binding activity. Acts upstream of or within response to bacterium. Located in cytosol. Is expressed in several structures, including alimentary system; ear; genitourinary system; limb mesenchyme; and musculoskeletal system. Orthologous to human CA3 (carbonic anhydrase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in subcutaneous fat pad adult (RPKM 4525.3), mammary gland adult (RPKM 3129.6) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 A1; 3 3.22 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (14928598..14937441)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (14863538..14872381)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CAIII transgenic hearts showed significantly less decrease in cardiac function than that of wild-type control animals.
Title: Transgenic expression of carbonic anhydrase III in cardiac muscle demonstrates a mechanism to tolerate acidosis.
these data clearly suggests that CAIII serves as an important antioxidant critical in protecting NP cells against oxidative stress-induced injury.
Title: Expression of Carbonic Anhydrase III, a Nucleus Pulposus Phenotypic Marker, is Hypoxia-responsive and Confers Protection from Oxidative Stress-induced Cell Death.
This study illustrates that skeletal muscle cell CAR3 is critical for CHRN homeostasis in the neuromuscular junction, and its deficiency leads to accelerated degradation of CHRN (cholinergic receptor nicotinic) and development of myasthenia gravis.
Title: Suppression of CHRN endocytosis by carbonic anhydrase CAR3 in the pathogenesis of myasthenia gravis.
CAIII is highly expressed in osteocytes, is regulated by parathyroid hormone both in vitro and in vivo, and protects osteocytes from oxidative stress.
Title: Carbonic anhydrase III protects osteocytes from oxidative stress.
Our results indicate that Car3 is not required for de novo lipogenesis, and Car3 knockout mice fed high-fat diet do not have significant differences in responses to various diets to wild type mice.
Title: Carbonic anhydrase III (Car3) is not required for fatty acid synthesis and does not protect against high-fat diet induced obesity in mice.
The results suggest that elevated isoaspartate and CPS-1, and reduced CA-III levels could serve as biomarkers of hepatocellular injury.
Title: Isoaspartate, carbamoyl phosphate synthase-1, and carbonic anhydrase-III as biomarkers of liver injury.
During inner ear development, transcripts of four cytosolic isozymes (Car1, Car2, Car3, and Car13), two membrane-bound isozymes (Car12 and Car14), and two CA-related proteins (Car8 and Car11) were expressed at higher levels than other isozymes.
Title: A systematic survey of carbonic anhydrase mRNA expression during mammalian inner ear development.
We conclude that down-regulation of CAIII in preadipocytes enhances adipogenesis and that CAIII is a regulator of adipogenic differentiation which acts at the level of PPAR-gamma2 gene expression.
Title: Carbonic anhydrase III regulates peroxisome proliferator-activated receptor-γ2.
Car2, Car3, Car7, and Car15 mRNAs were barely within the detection limits in the mouse harderian gland.
Title: Carbonic anhydrases in the mouse harderian gland.
lack of proximal tubule cLCN5 in mice and men is associated with CAIII induction, increased cell proliferation, and oxidative stress
Title: A novel renal carbonic anhydrase type III plays a role in proximal tubule dysfunction.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Car3 (e-PCR), detects polymorphism
- UniSTS:255279

Car3 (e-PCR), detects polymorphism
- UniSTS:506772

NoName (e-PCR)
- UniSTS:234647

BB219044 (e-PCR)
- UniSTS:185524

UniSTS:498421 (e-PCR)
- UniSTS:498421

Car3 (e-PCR), detects polymorphism
- UniSTS:498421

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables carbonate dehydratase activity	IBA Inferred from Biological aspect of Ancestor more info
enables carbonate dehydratase activity	ISO Inferred from Sequence Orthology more info
enables lyase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nickel cation binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in one-carbon metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within response to bacterium	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to oxidative stress	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: carbonic anhydrase 3

Names: CA-III; carbonate dehydratase III; carbonic anhydrase III

NP_031632.2

EC 4.2.1.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_007606.3 → NP_031632.2 carbonic anhydrase 3

See identical proteins and their annotated locations for NP_031632.2

Status: PROVISIONAL

Source sequence(s)

AC123747

Consensus CDS

CCDS17250.1

UniProtKB/Swiss-Prot

P16015, Q9ERN8

Related

ENSMUSP00000029076.5, ENSMUST00000029076.6

Conserved Domains (1) summary

cd03119
Location:1 → 259

alpha_CA_I_II_III_XIII; Carbonic anhydrase alpha, isozymes I, II, and III and XIII. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on ...