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    Aup1 ancient ubiquitous protein 1 [ Mus musculus (house mouse) ]

    Gene ID: 11993, updated on 11-Apr-2024

    Summary

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    Official Symbol
    Aup1provided by MGI
    Official Full Name
    ancient ubiquitous protein 1provided by MGI
    Primary source
    MGI:MGI:107789
    See related
    Ensembl:ENSMUSG00000068328 AllianceGenome:MGI:107789
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Summary
    The protein encoded by this gene contains several conserved domains including a hydrophobic domain, an acetyltransferase domain, a ubiquitin binding domain, and a domain required for recruitment of ubiquitin-conjugating enzyme E2 G2 (Ube2g2). In humans, this protein localizes to the endoplasmic reticulum and to lipid droplets. This protein is thought to be involved both in the degradation of misfolded proteins from the endoplasmic reticulum and in the storage of neutral lipids. Reduced expression of the human ortholog of this gene strongly reduces lipid droplet clustering in the cell, and causes stabilization of misfolded proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]
    Expression
    Ubiquitous expression in adrenal adult (RPKM 174.0), duodenum adult (RPKM 139.9) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    Location:
    6 C3; 6 35.94 cM
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (83031483..83034663)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (83054495..83057682)

    Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_16455 Neighboring gene docking protein 1 Neighboring gene lysyl oxidase-like 3 Neighboring gene STARR-positive B cell enhancer ABC_E2791 Neighboring gene microRNA 7040 Neighboring gene STARR-positive B cell enhancer ABC_E9275 Neighboring gene HtrA serine peptidase 2 Neighboring gene DEAQ RNA-dependent ATPase Neighboring gene T cell leukemia, homeobox 2

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Monoubiquitination of ancient ubiquitous protein 1 promotes lipid droplet clustering. Lohmann D, et al. PLoS One, 2013. PMID 24039768, Free PMC Article
    2. Aup1, a novel gene on mouse chromosome 6 and human chromosome 2p13. Jang W, et al. Genomics, 1996 Sep 1. PMID 8812468
    3. DQX1, an RNA-dependent ATPase homolog with a novel DEAQ box: expression pattern and genomic sequence comparison of the human and mouse genes. Ji W, et al. Mamm Genome, 2001 Jun. PMID 11353393
    4. Ancient ubiquitous protein-1 mediates sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl CoA reductase in lipid droplet-associated endoplasmic reticulum membranes. Jo Y, et al. Mol Biol Cell, 2013 Feb. PMID 23223569, Free PMC Article
    5. High-resolution genetic, physical, and transcript map of the mnd2 region of mouse chromosome 6. Weber JS, et al. Genomics, 1998 Nov 15. PMID 9806835

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables ubiquitin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin conjugating enzyme binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables ubiquitin protein ligase binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in ERAD pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ERAD pathway ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in lipid droplet formation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in lipid droplet organization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in lipophagy ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein localization to lipid droplet ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in response to virus ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in retrograde protein transport, ER to cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    located_in autophagosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in endoplasmic reticulum ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in endoplasmic reticulum membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in lipid droplet ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    lipid droplet-regulating VLDL assembly factor AUP1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001301649.1 → NP_001288578.1  lipid droplet-regulating VLDL assembly factor AUP1 isoform 2

      See identical proteins and their annotated locations for NP_001288578.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks an alternate exon in the coding region and uses a downstream start codon compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1.
      Source sequence(s)
      AK158035, BM900175, BY234324
      UniProtKB/TrEMBL
      I6L970
      Conserved Domains (2) summary
      cd14420
      Location:167 → 211
      CUE_AUP1; CUE domain found in ancient ubiquitous protein 1 (AUP1) and similar proteins
      cl17185
      Location:33 → 135
      LPLAT; Lysophospholipid acyltransferases (LPLATs) of glycerophospholipid biosynthesis

    2. NM_007517.5 → NP_031543.3  lipid droplet-regulating VLDL assembly factor AUP1 isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes the longer isoform (1).
      Source sequence(s)
      AC104324
      Consensus CDS
      CCDS39526.1
      UniProtKB/Swiss-Prot
      P70295
      UniProtKB/TrEMBL
      Q3TAZ9, Q3TBX5
      Related
      ENSMUSP00000090281.8, ENSMUST00000092618.10
      Conserved Domains (2) summary
      cd14420
      Location:296 → 340
      CUE_AUP1; CUE domain found in ancient ubiquitous protein 1 (AUP1) and similar proteins
      cl17185
      Location:66 → 264
      LPLAT; Lysophospholipid acyltransferases (LPLATs) of glycerophospholipid biosynthesis

    RNA

    1. NR_125891.1 RNA Sequence

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) uses an alternate splice site at an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
      Source sequence(s)
      AK158035, AK167335, BM900175, BY234324
      Related
      ENSMUST00000203915.4

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000072.7 Reference GRCm39 C57BL/6J

      Range
      83031483..83034663
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    RNA

    1. XR_377416.4 RNA Sequence

    2. XR_377415.4 RNA Sequence

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_001025446.1: Suppressed sequence

      Description
      NM_001025446.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P70295.1 GenPept UniProtKB/Swiss-Prot:P70295

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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