Alox8 arachidonate 8-lipoxygenase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Alox8provided by MGI
Official Full Name: arachidonate 8-lipoxygenaseprovided by MGI
Primary source: MGI:MGI:1098228
See related: Ensembl:ENSMUSG00000020891 AllianceGenome:MGI:1098228
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 8-LOX; 8S-LOX; Alox15b; 15-LOX-2; 15-LOX-B
Summary: This gene belongs to the lipoxygenase (LOX) gene family whose members encode enzymes that catalyze the addition of molecular oxygen to polyunsaturated fatty acids (PUFAs) to yield fatty acid hydroperoxides. The encoded enzyme preferentially metabolizes arachidonic acid to yield 8-hydroxyeicosatetraenoic acid (8-HETE), while metabolizing linoleic acid less efficiently. The gene may also function as a tumor suppressor. This gene is located in a cluster of related genes that spans approximately 75 kilobases on chromosome 11. [provided by RefSeq, Jan 2013]
Expression: Biased expression in cerebellum adult (RPKM 1.1), cortex adult (RPKM 0.5) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 B3; 11 42.38 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (69074711..69088669, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (69183885..69197843, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Humanization of the Reaction Specificity of Mouse Alox15b Inversely Modified the Susceptibility of Corresponding Knock-In Mice in Two Different Animal Inflammation Models.
Title: Humanization of the Reaction Specificity of Mouse Alox15b Inversely Modified the Susceptibility of Corresponding Knock-In Mice in Two Different Animal Inflammation Models.
Our findings, for the first time, strongly indicate that 1a may serve as a potent and promising cardioprotective agent in treatment of ischemia-reperfusion (I/R) injury, at least partially through targeting 12/15-LOX-2.
Title: Cardioprotection of (±)-sodium 5-bromo-2-(α-hydroxypentyl) benzoate (BZP) on mouse myocardium I/R injury through inhibiting 12/15-LOX-2 activity.
15-LOX-2 is distributed at the plasma membrane when cells are stimulated by the addition Ca(2+) ionophore and that cellular localization is dependent upon the presence of a putative membrane insertion loop.
Title: Membrane-dependent Activities of Human 15-LOX-2 and Its Murine Counterpart: IMPLICATIONS FOR MURINE MODELS OF ATHEROSCLEROSIS.
Knockdown of mouse Alox15b in vivo decreased plaque lipid content and markers of inflammation.
Title: Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis.
15-LOX2 expression in mouse prostate leads to hyperplasia and also induces cell senescence.
Title: Transgenic expression of 15-lipoxygenase 2 (15-LOX2) in mouse prostate leads to hyperplasia and cell senescence.
Expression of 8S-LOX and 15S-LOX-2 suppresses CRD-BP/IMP-1 expression, resulting in inhibition of human prostate carcinoma PC-3 cell proliferation.
Title: Tumor-suppressive lipoxygenases inhibit the expression of c-myc mRNA coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein 1 in human prostate carcinoma PC-3 cells.
The reaction specificities of wild-type vertebrate lipoxygenase isoforms are largely resistant towards pH alterations.
Title: Structural basis for pH-dependent alterations of reaction specificity of vertebrate lipoxygenase isoforms.
Purified recombinant 8S-lipoxygenase can function as a potential 15-LOX by converting arachidonic acid exclusively to 8S-HPETE and then to (8S,15S)-dihydroperoxy-5Z,9E,11Z,13E-eicosatetraenoic acid (8S,15S-diHPETE).
Title: Double dioxygenation by mouse 8S-lipoxygenase: specific formation of a potent peroxisome proliferator-activated receptor alpha agonist.
8S-LOX plays a role as a prodifferentiating, antitumorigenic, and tumor suppressing gene in mouse skin carcinogenesis.
Title: An antitumorigenic role for murine 8S-lipoxygenase in skin carcinogenesis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1)
Endonuclease-mediated (5)

General gene information

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Markers

Alox8 (e-PCR), detects polymorphism
- UniSTS:546650

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables arachidonate 15-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate 15-lipoxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables arachidonate 15-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables arachidonate 8(R)-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
enables arachidonate 8(S)-lipoxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables dioxygenase activity	IEA Inferred from Electronic Annotation more info
enables iron ion binding	ISO Inferred from Sequence Orthology more info
enables linoleate 13S-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables linoleate 9S-lipoxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in arachidonic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arachidonic acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in arachidonic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in cannabinoid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in endocannabinoid signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in hepoxilin biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in hepoxilin biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in linoleic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in linoleic acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in lipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in lipid oxidation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipoxin A4 biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in lipoxygenase pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipoxygenase pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in lipoxygenase pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell cycle	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of growth	ISO Inferred from Sequence Orthology more info
involved_in phospholipid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phospholipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of chemokine production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of keratinocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of macrophage derived foam cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peroxisome proliferator activated receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in adherens junction	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: polyunsaturated fatty acid lipoxygenase ALOX8

Names: 15-lipoxygenase 2; 8S-lipoxygenase; arachidonate 15-lipoxygenase B; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type; arachidonate 8S-lipoxygenase; linoleate 9S-lipoxygenase ALOX8

NP_033791.1

EC 1.13.11.58

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009661.4 → NP_033791.1 polyunsaturated fatty acid lipoxygenase ALOX8

See identical proteins and their annotated locations for NP_033791.1

Status: REVIEWED

Source sequence(s)

AK028724, AL645527

Consensus CDS

CCDS24886.1

UniProtKB/Swiss-Prot

B1ASX5, O35936

UniProtKB/TrEMBL

B1ASX6

Related

ENSMUSP00000021262.4, ENSMUST00000021262.10

Conserved Domains (2) summary

cd01753
Location:5 → 122

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:230 → 668

Lipoxygenase; Lipoxygenase