Adam8 a disintegrin and metallopeptidase domain 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Adam8provided by MGI
Official Full Name: a disintegrin and metallopeptidase domain 8provided by MGI
Primary source: MGI:MGI:107825
See related: Ensembl:ENSMUSG00000025473 AllianceGenome:MGI:107825
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: MS2; CD156; ADAM 8; CD156a; E430039A18Rik
Summary: This gene encodes a member of the Adam family of proteins that contain the disintegrin and metalloprotease domains. The encoded protein is localized to the cell surface, where it is involved in the remodeling of extracellular matrix and cell migration. Mice lacking the encoded protein display persistent inflammation upon treatment with allergens. Alternative splicing of this gene results in multiple variants. [provided by RefSeq, Mar 2015]
Expression: Broad expression in ovary adult (RPKM 21.0), thymus adult (RPKM 6.6) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 F4; 7 84.96 cM

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (139558845..139573016, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (139978932..139995207, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Knockdown of ADAM8 inhibits the proliferation, migration, invasion, and tumorigenesis of renal clear cell carcinoma cells to enhance the immunotherapy efficacy.
Title: Knockdown of ADAM8 inhibits the proliferation, migration, invasion, and tumorigenesis of renal clear cell carcinoma cells to enhance the immunotherapy efficacy.
Expression of the Metalloproteinase ADAM8 Is Upregulated in Liver Inflammation Models and Enhances Cytokine Release In Vitro.
Title: Expression of the Metalloproteinase ADAM8 Is Upregulated in Liver Inflammation Models and Enhances Cytokine Release In Vitro.
ADAM8 signaling drives neutrophil migration and ARDS severity.
Title: ADAM8 signaling drives neutrophil migration and ARDS severity.
Suppression of ADAM8 attenuates angiotensin II-induced cardiac fibrosis and endothelial-mesenchymal transition via inhibiting TGF-beta1/Smad2/Smad3 pathways.
Title: Suppression of ADAM8 attenuates angiotensin II-induced cardiac fibrosis and endothelial-mesenchymal transition via inhibiting TGF-β1/Smad2/Smad3 pathways.
Elevated inflammatory gene expression in intervertebral disc tissues in mice with ADAM8 inactivated.
Title: Elevated inflammatory gene expression in intervertebral disc tissues in mice with ADAM8 inactivated.
Expression levels of the metalloproteinase ADAM8 critically regulate proliferation, migration and malignant signalling events in hepatoma cells.
Title: Expression levels of the metalloproteinase ADAM8 critically regulate proliferation, migration and malignant signalling events in hepatoma cells.
ADAMs binds to integrins to regulate cell adhesion and migration.
Title: A Disintegrin and Metalloproteinase Domain-8: A Novel Protective Proteinase in Chronic Obstructive Pulmonary Disease.
In mice, Adam8 was highly expressed in circulating neutrophils and in macrophages. Moreover, ADAM8 deficient mouse macrophages displayed reduced secretion of inflammatory mediators. Remarkably, however, neither hematopoietic nor whole-body ADAM8 deficiency in mice affected atherosclerotic lesion size.
Title: Whole body and hematopoietic ADAM8 deficiency does not influence advanced atherosclerotic lesion development, despite its association with human plaque progression.
Gene expression of adam8 was elevated at all time points after injury.
Title: Intervertebral Disc Degeneration in a Percutaneous Mouse Tail Injury Model.
ADAM8 on leukocytes holds a proinflammatory function in acute lung inflammation by promoting alveolar leukocyte recruitment.
Title: The metalloproteinase ADAM8 promotes leukocyte recruitment in vitro and in acute lung inflammation.

Submit: New GeneRIF Correction See all GeneRIFs (33)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Endonuclease-mediated (5)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables cell adhesion molecule binding	IBA Inferred from Biological aspect of Ancestor more info
enables cell adhesion molecule binding	IPI Inferred from Physical Interaction more info	PubMed
enables cell adhesion molecule binding	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables integrin binding	TAS Traceable Author Statement more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloendopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metallopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables metallopeptidase activity	ISO Inferred from Sequence Orthology more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in angiogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in blood vessel endothelial cell migration	NAS Non-traceable Author Statement more info	PubMed
involved_in canonical NF-kappaB signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell-cell adhesion	IDA Inferred from Direct Assay more info	PubMed
involved_in cell-matrix adhesion	TAS Traceable Author Statement more info	PubMed
involved_in cellular response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in inflammatory response	IBA Inferred from Biological aspect of Ancestor more info
involved_in inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in leukocyte migration involved in inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lymphocyte chemotaxis	IDA Inferred from Direct Assay more info	PubMed
involved_in membrane fusion	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of neuron apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of thymocyte aggregation	NAS Non-traceable Author Statement more info	PubMed
involved_in osteoclast fusion	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of MAPK cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of T cell differentiation in thymus	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of T cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of acute inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of bone resorption	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of bone resorption	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell adhesion	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cellular extravasation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of cellular extravasation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of eosinophil migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of epithelial to mesenchymal transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of fibronectin-dependent thymocyte migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of inflammatory response	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of innate immune response	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of membrane protein ectodomain proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of membrane protein ectodomain proteolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of membrane protein ectodomain proteolysis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neutrophil extravasation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of thymocyte apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of tumor necrosis factor (ligand) superfamily member 11 production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cell-cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cell-cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in regulation of osteoclast differentiation	NAS Non-traceable Author Statement more info	PubMed
involved_in tissue morphogenesis	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of alpha9-beta1 integrin-ADAM8 complex	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in dense core granule membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in phagolysosome	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in podosome	ISO Inferred from Sequence Orthology more info
located_in podosome	TAS Traceable Author Statement more info	PubMed
located_in specific granule	ISO Inferred from Sequence Orthology more info
located_in tertiary granule	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: disintegrin and metalloproteinase domain-containing protein 8

Names: a disintegrin and metalloprotease domain 8; cell surface antigen MS2; macrophage cysteine-rich glycoprotein

NP_001277995.1

EC 3.4.24.-

NP_031429.1

EC 3.4.24.-

XP_006536214.1

EC 3.4.24.-

XP_017177421.1

EC 3.4.24.-

XP_017177422.1

EC 3.4.24.-

XP_017177423.1

EC 3.4.24.-

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001291066.3 → NP_001277995.1 disintegrin and metalloproteinase domain-containing protein 8 isoform 2 precursor

See identical proteins and their annotated locations for NP_001277995.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice acceptor site compared to variant 1. It encodes a protein (isoform 2) that is slightly shorter than isoform 1.

Source sequence(s)

AC107822

Consensus CDS

CCDS72064.1

UniProtKB/TrEMBL

Q3TVN5, Q3U1R3, Q3U6N3, Q3U7G2, Q8R3D3

Related

ENSMUSP00000026546.10, ENSMUST00000026546.16

Conserved Domains (5) summary

smart00050
Location:411 → 485

DISIN; Homologues of snake disintegrins

smart00608
Location:487 → 606

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:195 → 392

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam01421
Location:195 → 394

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:49 → 148

Pep_M12B_propep; Reprolysin family propeptide
NM_007403.4 → NP_031429.1 disintegrin and metalloproteinase domain-containing protein 8 isoform 1 precursor

See identical proteins and their annotated locations for NP_031429.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer protein isoform (1).

Source sequence(s)

AC107822

Consensus CDS

CCDS21959.1

UniProtKB/Swiss-Prot

Q05910

UniProtKB/TrEMBL

Q3TVN5, Q3U1J7, Q3U1R3, Q3U6N3, Q8R3D3

Related

ENSMUSP00000101684.3, ENSMUST00000106069.9

Conserved Domains (5) summary

smart00050
Location:412 → 486

DISIN; Homologues of snake disintegrins

smart00608
Location:488 → 607

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:196 → 393

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam01421
Location:196 → 395

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:50 → 149

Pep_M12B_propep; Reprolysin family propeptide

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

139558845..139573016 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017321933.3 → XP_017177422.1 disintegrin and metalloproteinase domain-containing protein 8 isoform X2

UniProtKB/TrEMBL

Q3TVN5, Q3U1R3, Q3U6N3, Q8R3D3

Conserved Domains (6) summary

smart00050
Location:411 → 485

DISIN; Homologues of snake disintegrins

smart00608
Location:487 → 606

ACR; ADAM Cysteine-Rich Domain

PHA03247
Location:637 → 856

PHA03247; large tegument protein UL36; Provisional

pfam01421
Location:195 → 394

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:27 → 151

Pep_M12B_propep; Reprolysin family propeptide

pfam05104
Location:770 → 855

Rib_recp_KP_reg; Ribosome receptor lysine/proline rich region
XM_006536151.4 → XP_006536214.1 disintegrin and metalloproteinase domain-containing protein 8 isoform X3

UniProtKB/TrEMBL

Q3TVN5, Q3U1R3, Q3U6N3, Q8R3D3

Conserved Domains (5) summary

smart00050
Location:412 → 486

DISIN; Homologues of snake disintegrins

smart00608
Location:488 → 607

ACR; ADAM Cysteine-Rich Domain

pfam01421
Location:196 → 395

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:28 → 152

Pep_M12B_propep; Reprolysin family propeptide

pfam05104
Location:766 → 851

Rib_recp_KP_reg; Ribosome receptor lysine/proline rich region
XM_017321932.3 → XP_017177421.1 disintegrin and metalloproteinase domain-containing protein 8 isoform X1

UniProtKB/TrEMBL

Q3TVN5, Q3U1R3, Q3U6N3, Q8R3D3

Conserved Domains (6) summary

smart00050
Location:412 → 486

DISIN; Homologues of snake disintegrins

smart00608
Location:488 → 607

ACR; ADAM Cysteine-Rich Domain

PHA03247
Location:638 → 857

PHA03247; large tegument protein UL36; Provisional

pfam01421
Location:196 → 395

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:28 → 152

Pep_M12B_propep; Reprolysin family propeptide

pfam05104
Location:771 → 856

Rib_recp_KP_reg; Ribosome receptor lysine/proline rich region
XM_017321934.3 → XP_017177423.1 disintegrin and metalloproteinase domain-containing protein 8 isoform X4

UniProtKB/TrEMBL

E9Q359, Q8C269

Related

ENSMUSP00000117858.2, ENSMUST00000148670.8

Conserved Domains (4) summary

smart00050
Location:412 → 486

DISIN; Homologues of snake disintegrins

smart00608
Location:488 → 607

ACR; ADAM Cysteine-Rich Domain

pfam01421
Location:196 → 395

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:28 → 152

Pep_M12B_propep; Reprolysin family propeptide