Ada adenosine deaminase [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Adaprovided by MGI
Official Full Name: adenosine deaminaseprovided by MGI
Primary source: MGI:MGI:87916
See related: Ensembl:ENSMUSG00000017697 AllianceGenome:MGI:87916
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables 2'-deoxyadenosine deaminase activity; adenosine deaminase activity; and zinc ion binding activity. Involved in allantoin metabolic process and purine nucleoside monophosphate metabolic process. Acts upstream of or within several processes, including animal organ development; nucleobase-containing small molecule metabolic process; and positive regulation of lymphocyte activation. Located in cytoplasm. Is active in cytosol. Is expressed in several structures, including alimentary system; brain; extraembryonic component; genitourinary system; and sensory organ. Used to study adenosine deaminase deficiency. Human ortholog(s) of this gene implicated in adenosine deaminase deficiency; asthma; and severe combined immunodeficiency. Orthologous to human ADA (adenosine deaminase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in small intestine adult (RPKM 276.4), placenta adult (RPKM 134.5) and 4 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 84.44 cM; 2 H3

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (163565703..163592154, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (163726571..163750239, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inhibition of eADA blocked endothelial activation suggesting a crucial role of this enzyme in the control of vascular inflammation. This supports the concept of eADA targeted vascular protection therapy
Title: Inhibition of LPS-stimulated ecto-adenosine deaminase attenuates endothelial cell activation.
Pulmonary alveolar proteinosis in adenosine deaminase-deficient mice
Title: Pulmonary alveolar proteinosis in adenosine deaminase-deficient mice.
Dietary regulation of adenosine deaminase activity in stomach, small intestine and spleen of mice
Title: Dietary regulation of adenosine deaminase activity in stomach, small intestine and spleen of mice.
Data indicate that untreated murine adenosine deaminase ADA(-/-) Tregs show alterations in the plasma membrane CD39/CD73. ectonucleotidase machinery and limited suppressive activity via extracellular adenosine.
Title: Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID.
ADA is upregulated in NOD dendritic cells, which results in their exuberant and spontaneous activation, which triggers autoimmune T cell activation.
Title: High levels of adenosine deaminase on dendritic cells promote autoreactive T cell activation and diabetes in nonobese diabetic mice.
The catalytic mechanism of Mus musculus adenosine deaminase (ADA) has been studied by quantum mechanics and two-layered ONIOM calculations
Title: A theoretical study on the catalytic mechanism of Mus musculus adenosine deaminase.
Adenosine deaminase is necessary but not sufficient to confer puromycin aminonucleoside sensitivity to podocytes.
Title: Over-expression of adenosine deaminase in mouse podocytes does not reverse puromycin aminonucleoside resistance.
ADA-deficient SCID is associated with a specific microenvironment and bone phenotype characterized by RANKL/OPG imbalance and osteoblast insufficiency.
Title: ADA-deficient SCID is associated with a specific microenvironment and bone phenotype characterized by RANKL/OPG imbalance and osteoblast insufficiency.
ADA/A2B adenosine receptor double-knockout mice exhibited enhanced pulmonary inflammation and airway destruction
Title: Enhanced airway inflammation and remodeling in adenosine deaminase-deficient mice lacking the A2B adenosine receptor.
Progesterone may have an antiseizure effect with the additional finding that decreased levels of adenosine deaminase that would have resulted in increased adenosine levels that exerts anticonvulsant effect via GABA-A receptors.
Title: Effects of progesterone on total brain tissue adenosine deaminase activity in experimental epilepsy.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Not Applicable (1)
Endonuclease-mediated (3)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Ada (e-PCR), detects polymorphism
- UniSTS:506710

M10319 (e-PCR)
- UniSTS:159066

Ada (e-PCR), detects polymorphism
- UniSTS:141024

Ada (e-PCR), detects polymorphism
- UniSTS:141025

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 2'-deoxyadenosine deaminase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables 2'-deoxyadenosine deaminase activity	ISO Inferred from Sequence Orthology more info
enables adenosine deaminase activity	IBA Inferred from Biological aspect of Ancestor more info
enables adenosine deaminase activity	IDA Inferred from Direct Assay more info	PubMed
enables adenosine deaminase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables adenosine deaminase activity	ISO Inferred from Sequence Orthology more info
enables deaminase activity	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables purine nucleoside binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in AMP catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in AMP salvage	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of B cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in GMP salvage	IDA Inferred from Direct Assay more info	PubMed
involved_in IMP salvage	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within Peyer's patch development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in T cell activation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of T cell activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in T cell activation	ISO Inferred from Sequence Orthology more info
acts_upstream_of T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of T cell differentiation in thymus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of T cell receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in adenosine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within adenosine catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in adenosine catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within adenosine catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within adenosine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in adenosine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in adenosine metabolic process	ISO Inferred from Sequence Orthology more info	PubMed
involved_in allantoin metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in allantoin metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of alpha-beta T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in amide catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in amide catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of calcium-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in dAMP catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within dATP catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within dATP catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within deoxyadenosine catabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within deoxyadenosine catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in deoxyadenosine catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within embryonic digestive tract development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within germinal center B cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of germinal center formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in histamine secretion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within hypoxanthine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hypoxanthine salvage	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within in utero embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in inhibition of non-skeletal tissue mineralization	ISO Inferred from Sequence Orthology more info	PubMed
involved_in inosine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within inosine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in inosine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within inosine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within inosine biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of leukocyte migration	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of leukocyte migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within liver development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lung alveolus development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lung development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of mature B cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of mucus secretion	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of mucus secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of adenosine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of adenosine receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of circadian sleep/wake cycle, non-REM sleep	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of inflammatory response	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of leukocyte migration	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of leukocyte migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of mature B cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of mucus secretion	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of mucus secretion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of penile erection	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of thymocyte apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within nucleotide metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of penile erection	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within placenta development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of B cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of T cell activation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of T cell differentiation in thymus	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of T cell receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of alpha-beta T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of calcium-mediated signaling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of germinal center formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of heart rate	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in purine nucleotide salvage	ISO Inferred from Sequence Orthology more info
involved_in purine ribonucleoside monophosphate biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of T cell differentiation in thymus	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell-cell adhesion mediated by integrin	ISO Inferred from Sequence Orthology more info
involved_in response to hypoxia	ISO Inferred from Sequence Orthology more info
involved_in response to inorganic substance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to inorganic substance	ISO Inferred from Sequence Orthology more info	PubMed
involved_in response to purine-containing compound	ISO Inferred from Sequence Orthology more info	PubMed
involved_in response to vitamin E	ISO Inferred from Sequence Orthology more info
acts_upstream_of smooth muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of thymocyte apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within trophectodermal cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within xanthine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in anchoring junction	IEA Inferred from Electronic Annotation more info
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendrite cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in external side of plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in lysosome	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: adenosine deaminase

Names: adenosine aminohydrolase

NP_001258981.1

EC 3.5.4.4

NP_001403804.1

EC 3.5.4.4

NP_001403805.1

EC 3.5.4.4

NP_031424.1

EC 3.5.4.4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001272052.2 → NP_001258981.1 adenosine deaminase isoform 1

See identical proteins and their annotated locations for NP_001258981.1

Status: VALIDATED

Source sequence(s)

AL591490

Consensus CDS

CCDS17015.1

UniProtKB/Swiss-Prot

P03958

UniProtKB/TrEMBL

Q4FJZ7, Q4FK28

Conserved Domains (1) summary

cd01320
Location:8 → 336

ADA; Adenosine deaminase (ADA) is a monomeric zinc dependent enzyme which catalyzes the irreversible hydrolytic deamination of both adenosine, as well as desoxyadenosine, to ammonia and inosine or desoxyinosine, respectively. ADA plays an important role in ...
NM_001416875.1 → NP_001403804.1 adenosine deaminase isoform 2

Status: VALIDATED

Source sequence(s)

AL591490
NM_001416876.1 → NP_001403805.1 adenosine deaminase isoform 4

Status: VALIDATED

Source sequence(s)

AL591490
NM_007398.5 → NP_031424.1 adenosine deaminase isoform 1

See identical proteins and their annotated locations for NP_031424.1

Status: VALIDATED

Source sequence(s)

AL591490

Consensus CDS

CCDS17015.1

UniProtKB/Swiss-Prot

P03958

UniProtKB/TrEMBL

Q4FJZ7, Q4FK28

Related

ENSMUSP00000017841.4, ENSMUST00000017841.4

Conserved Domains (1) summary

cd01320
Location:8 → 336

ADA; Adenosine deaminase (ADA) is a monomeric zinc dependent enzyme which catalyzes the irreversible hydrolytic deamination of both adenosine, as well as desoxyadenosine, to ammonia and inosine or desoxyinosine, respectively. ADA plays an important role in ...