U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination
    • Showing Current items.

    Rnf216 ring finger protein 216 [ Mus musculus (house mouse) ]

    Gene ID: 108086, updated on 5-Mar-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    Rnf216provided by MGI
    Official Full Name
    ring finger protein 216provided by MGI
    Primary source
    MGI:MGI:1344349
    See related
    Ensembl:ENSMUSG00000045078 AllianceGenome:MGI:1344349
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    UIP83; TRIAD3; Ubce7ip1; 2810055G22Rik; F830018F18Rik
    Summary
    Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in several processes, including protein K48-linked ubiquitination; protein catabolic process; and regulation of defense response to virus by host. Predicted to act upstream of or within apoptotic process. Predicted to be located in cytosol; nucleoplasm; and synapse. Human ortholog(s) of this gene implicated in Gordon Holmes syndrome. Orthologous to human RNF216 (ring finger protein 216). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in testis adult (RPKM 22.7), adrenal adult (RPKM 18.3) and 28 other tissues See more
    Orthologs
    human all
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Location:
    5 G2; 5 81.86 cM
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 5 NC_000071.7 (142976648..143098793, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 5 NC_000071.6 (142990893..143113041, complement)

    Chromosome 5 - NC_000071.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E6395 Neighboring gene STARR-positive B cell enhancer ABC_E674 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:143670916-143671255 Neighboring gene predicted gene, 38733 Neighboring gene CapStarr-seq enhancer MGSCv37_chr5:143682513-143682666 Neighboring gene actin, beta Neighboring gene STARR-seq mESC enhancer starr_14775 Neighboring gene STARR-seq mESC enhancer starr_14777 Neighboring gene fascin actin-bundling protein 1 Neighboring gene STARR-seq mESC enhancer starr_14779 Neighboring gene VISTA enhancer mm1619 Neighboring gene predicted gene 43380 Neighboring gene STARR-seq mESC enhancer starr_14782 Neighboring gene STARR-seq mESC enhancer starr_14783 Neighboring gene STARR-positive B cell enhancer ABC_E3631 Neighboring gene STARR-seq mESC enhancer starr_14784 Neighboring gene nonconserved acetylation island sequence M61 enhancer Neighboring gene olfactory receptor family 10 subfamily AH member 1, pseudogene 1 Neighboring gene predicted gene, 38734 Neighboring gene STARR-seq mESC enhancer starr_14788 Neighboring gene predicted gene 7291

    Genomic regions, transcripts, and products

    Go to the top of the page Help

    Go to nucleotide: Graphics FASTA GenBank

    Expression

    Go to the top of the page Help
    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. The E3 ubiquitin ligase RNF216/TRIAD3 is a key coordinator of the hypothalamic-pituitary-gonadal axis. George AJ, et al. iScience, 2022 Jun 17. PMID 35620441, Free PMC Article
    2. Triad3A displays a critical role in suppression of cerebral ischemic/reperfusion (I/R) injury by regulating necroptosis. Yuan Z, et al. Biomed Pharmacother, 2020 Aug. PMID 32460187
    3. Triad3A attenuates pathological cardiac hypertrophy involving the augmentation of ubiquitination-mediated degradation of TLR4 and TLR9. Lu X, et al. Basic Res Cardiol, 2020 Feb 1. PMID 32008145
    4. RNF216 is essential for spermatogenesis and male fertility†. Melnick AF, et al. Biol Reprod, 2019 May 1. PMID 30649198, Free PMC Article
    5. Triad3a induces the degradation of early necrosome to limit RipK1-dependent cytokine production and necroptosis. Alturki NA, et al. Cell Death Dis, 2018 May 22. PMID 29789521, Free PMC Article

    See all (30) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. RNF216 affects the stability of STAU2 in the hypothalamus.
      Title: RNF216 affects the stability of STAU2 in the hypothalamus.
    2. RNF216 regulates meiosis and PKA stability in the testes.
      Title: RNF216 regulates meiosis and PKA stability in the testes.
    3. Triad3A displays a critical role in suppression of cerebral ischemic/reperfusion (I/R) injury by regulating necroptosis.
      Title: Triad3A displays a critical role in suppression of cerebral ischemic/reperfusion (I/R) injury by regulating necroptosis.
    4. Triad3A attenuates pathological cardiac hypertrophy involving the augmentation of ubiquitination-mediated degradation of TLR4 and TLR9.
      Title: Triad3A attenuates pathological cardiac hypertrophy involving the augmentation of ubiquitination-mediated degradation of TLR4 and TLR9.
    5. An essential function of RNF216 in spermatogenesis and male fertility and suggest a critical role for RNF216 in human gonadal development.
      Title: RNF216 is essential for spermatogenesis and male fertility†.
    6. Triad3a promotes the negative feedback loop independently of typical RipK1 ubiquitin editing enzymes
      Title: Triad3a induces the degradation of early necrosome to limit RipK1-dependent cytokine production and necroptosis.
    7. Low RNF216 expression is associated with obesity-induced inflammation.
      Title: Green Tea Polyphenol Epigallocatechin-3-gallate Suppresses Toll-like Receptor 4 Expression via Up-regulation of E3 Ubiquitin-protein Ligase RNF216.
    8. missense mutations in TRIAD3 abolished the interaction of TRIAD3A with Arc, disrupting Arc ubiquitination, and consequently Arc degradation.
      Title: TRIAD3/RNF216 mutations associated with Gordon Holmes syndrome lead to synaptic and cognitive impairments via Arc misregulation.
    9. identifies a novel negative regulator of autophagy and suggests that RNF216 may be a target for treatment of inflammatory diseases
      Title: Regulation of autophagy by E3 ubiquitin ligase RNF216 through BECN1 ubiquitination.
    Submit: New GeneRIF Correction

    Variation

    Go to the top of the page Help

    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Targeted (3) 
    • Endonuclease-mediated (3)  1 citation

    Pathways from PubChem

    Go to the top of the page

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin protein ligase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within apoptotic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proteasome-mediated ubiquitin-dependent protein catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein K48-linked ubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein catabolic process, modulating synaptic transmission ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of defense response to virus by host ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of interferon-beta production ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of postsynaptic neurotransmitter receptor internalization ISO
    Inferred from Sequence Orthology
    more info
    		  
    Component Evidence Code Pubs
    is_active_in Schaffer collateral - CA1 synapse ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasmic vesicle IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in glutamatergic synapse ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in postsynaptic endocytic zone ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in presynapse ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in synapse ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    E3 ubiquitin-protein ligase RNF216
    Names
    E3 ubiquitin ligase
    RING-type E3 ubiquitin transferase RNF216
    triad domain-containing protein 3
    ubcM4-interacting protein 83
    ubiquitin-conjugating enzyme 7-interacting protein 1
    NP_001346780.1
    NP_542128.2
    NP_996993.1
    XP_006504707.1
    XP_006504708.1
    XP_006504709.1
    XP_017176080.1
    XP_017176081.1
    XP_017176082.1
    XP_030109902.1
    XP_036020615.1
    XP_036020616.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001359851.1 → NP_001346780.1  E3 ubiquitin-protein ligase RNF216 isoform C

      Status: VALIDATED

      Description
      Transcript Variant: This variant (C) differs in the 5' UTR and coding sequence compared to variant B. The resulting isoform (C) is shorter at the N-terminus compared to isoform B.
      Source sequence(s)
      AC113281
      Conserved Domains (1) summary
      cd16630
      Location:310 → 366
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

    2. NM_080561.4 → NP_542128.2  E3 ubiquitin-protein ligase RNF216 isoform A

      See identical proteins and their annotated locations for NP_542128.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (A) uses an alternate in-frame splice junction compared to variant B. The resulting isoform (A) has the same N- and C-termini but is shorter compared to isoform B.
      Source sequence(s)
      BB854046, BC065066
      Consensus CDS
      CCDS80455.1
      UniProtKB/Swiss-Prot
      P58283, Q3U493, Q3UE56, Q3UGM3, Q68FN0, Q6P1H8, Q6PWY5, Q8BN27, Q8C1U3
      Related
      ENSMUSP00000052563.10, ENSMUST00000053498.13
      Conserved Domains (2) summary
      cd16630
      Location:501 → 557
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
      pfam01485
      Location:643 → 693
      IBR; IBR domain, a half RING-finger domain

    3. NM_207110.1 → NP_996993.1  E3 ubiquitin-protein ligase RNF216 isoform B

      See identical proteins and their annotated locations for NP_996993.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (B) represents the longest transcript and encodes the longest isoform (B).
      Source sequence(s)
      AC113281, AK147861, BB854046, BC065066
      Consensus CDS
      CCDS57400.1
      UniProtKB/Swiss-Prot
      P58283
      Related
      ENSMUSP00000143705.2, ENSMUST00000200607.5
      Conserved Domains (2) summary
      cd16630
      Location:558 → 614
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
      pfam01485
      Location:700 → 750
      IBR; IBR domain, a half RING-finger domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000071.7 Reference GRCm39 C57BL/6J

      Range
      142976648..143098793 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030254042.1 → XP_030109902.1  E3 ubiquitin-protein ligase RNF216 isoform X4

      Conserved Domains (2) summary
      cd16630
      Location:416 → 472
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
      pfam01485
      Location:558 → 608
      IBR; IBR domain, a half RING-finger domain

    2. XM_006504646.4 → XP_006504709.1  E3 ubiquitin-protein ligase RNF216 isoform X3

      Conserved Domains (2) summary
      cd16630
      Location:473 → 529
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
      pfam01485
      Location:615 → 665
      IBR; IBR domain, a half RING-finger domain

    3. XM_006504644.5 → XP_006504707.1  E3 ubiquitin-protein ligase RNF216 isoform X1

      See identical proteins and their annotated locations for XP_006504707.1

      UniProtKB/Swiss-Prot
      P58283
      Conserved Domains (2) summary
      cd16630
      Location:558 → 614
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
      pfam01485
      Location:700 → 750
      IBR; IBR domain, a half RING-finger domain

    4. XM_006504645.5 → XP_006504708.1  E3 ubiquitin-protein ligase RNF216 isoform X2

      See identical proteins and their annotated locations for XP_006504708.1

      UniProtKB/Swiss-Prot
      P58283, Q3U493, Q3UE56, Q3UGM3, Q68FN0, Q6P1H8, Q6PWY5, Q8BN27, Q8C1U3
      Conserved Domains (2) summary
      cd16630
      Location:501 → 557
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
      pfam01485
      Location:643 → 693
      IBR; IBR domain, a half RING-finger domain

    5. XM_017320593.3 → XP_017176082.1  E3 ubiquitin-protein ligase RNF216 isoform X5

      Conserved Domains (1) summary
      cd16630
      Location:310 → 366
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

    6. XM_017320592.3 → XP_017176081.1  E3 ubiquitin-protein ligase RNF216 isoform X5

      Conserved Domains (1) summary
      cd16630
      Location:310 → 366
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

    7. XM_036164722.1 → XP_036020615.1  E3 ubiquitin-protein ligase RNF216 isoform X1

      Conserved Domains (2) summary
      cd16630
      Location:558 → 614
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
      pfam01485
      Location:700 → 750
      IBR; IBR domain, a half RING-finger domain

    8. XM_017320591.3 → XP_017176080.1  E3 ubiquitin-protein ligase RNF216 isoform X1

      UniProtKB/Swiss-Prot
      P58283
      Conserved Domains (2) summary
      cd16630
      Location:558 → 614
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
      pfam01485
      Location:700 → 750
      IBR; IBR domain, a half RING-finger domain

    9. XM_036164723.1 → XP_036020616.1  E3 ubiquitin-protein ligase RNF216 isoform X2

      UniProtKB/Swiss-Prot
      P58283, Q3U493, Q3UE56, Q3UGM3, Q68FN0, Q6P1H8, Q6PWY5, Q8BN27, Q8C1U3
      Conserved Domains (2) summary
      cd16630
      Location:501 → 557
      RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
      pfam01485
      Location:643 → 693
      IBR; IBR domain, a half RING-finger domain
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P58283.3 GenPept UniProtKB/Swiss-Prot:P58283

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Chemical Information
    Molecular Biology Databases
    Research Materials
    Tools
    Miscellaneous