Isl2 insulin related protein 2 (islet 2) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Isl2provided by MGI
Official Full Name: insulin related protein 2 (islet 2)provided by MGI
Primary source: MGI:MGI:109156
See related: Ensembl:ENSMUSG00000032318 AllianceGenome:MGI:109156
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 3110001N10Rik
Summary: Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within negative regulation of neuron differentiation and neuron differentiation. Located in nucleus. Is expressed in several structures, including central nervous system; genitourinary system; limb ectoderm; retina; and spinal ganglion. Orthologous to human ISL2 (ISL LIM homeobox 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in CNS E11.5 (RPKM 2.3), colon adult (RPKM 1.4) and 11 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 B; 9 30.13 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (55445961..55454951)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (55538677..55547667)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Transcriptional control of motor pool formation and motor circuit connectivity by the LIM-HD protein Isl2.
Title: Transcriptional control of motor pool formation and motor circuit connectivity by the LIM-HD protein Isl2.
We demonstrate that mutation of a solvent exposed side chain in the spacer region of an LHX4-ISL2 complex has no significant effect on the structure of the complex, but decreases binding affinity, apparently by increasing flexibility of the linker.
Title: Mutation in a flexible linker modulates binding affinity for modular complexes.
lack both Isl1 and Isl2, large numbers of motor neurons exited the neural tube, even prior to the appearance of boundary cap cells at the ventral exit points
Title: Slit and Semaphorin signaling governed by Islet transcription factors positions motor neuron somata within the neural tube.
X-ray crystal- and small-angle x-ray scattering-derived solution structures of an Lhx4.Isl2 complex exhibit many similarities with that of Lhx3.Isl1; structural differences supported by mutagenic studies reveal differences in the mechanisms of binding.
Title: Structural basis for partial redundancy in a class of transcription factors, the LIM homeodomain proteins, in neural cell type specification.
Data describe how the dosage of LIM homeodomain proteins Isl1 and Isl2 and LIM-only protein Lmo4 influences the assembly and function of complexes involved in the generation of spinal motor neurons (MNs) and V2a interneurons (INs).
Title: Islet-to-LMO stoichiometries control the function of transcription complexes that specify motor neuron and V2a interneuron identity.
Successful purification and crystallization of an engineered complex comprising the LIM domains of motor neuron protein Lhx4 and the Lim binding domain of Isl2 (islet 2 protein) are reported.
Title: Crystallization and diffraction of an Lhx4-Isl2 complex.
The second wave of expression at E12.5 includes Lhx3, 5, 9, Isl2, and Lmx1b in the differentiating sensory epithelia with cellular specificities.
Title: Diverse expression patterns of LIM-homeodomain transcription factors (LIM-HDs) in mammalian inner ear development.
Isl2 specifies RGC laterality by repressing an ipsilateral pathfinding program unique to VTC RGCs and involving Zic2 and EphB1. This genetic hierarchy controls binocular vision
Title: Magnitude of binocular vision controlled by islet-2 repression of a genetic program that specifies laterality of retinal axon pathfinding.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Isl2 (e-PCR), detects polymorphism
- UniSTS:527396

NoName (e-PCR)
- UniSTS:490819

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in axonogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of neuron differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of neuron differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuron fate commitment	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within neuron fate commitment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron fate specification	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within retinal ganglion cell axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spinal cord motor neuron cell fate specification	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within visceral motor neuron differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within visceral motor neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: insulin gene enhancer protein ISL-2

Names: islet 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_027397.3 → NP_081673.2 insulin gene enhancer protein ISL-2

See identical proteins and their annotated locations for NP_081673.2

Status: VALIDATED

Source sequence(s)

AC116699, AK013964, BC120880, BE953935

Consensus CDS

CCDS23205.1

UniProtKB/Swiss-Prot

G5E888, Q9CXV0

Related

ENSMUSP00000034869.5, ENSMUST00000034869.11

Conserved Domains (3) summary

cd09366
Location:27 → 81

LIM1_Isl; The first LIM domain of Isl, a member of LHX protein family

cd09471
Location:89 → 143

LIM2_Isl2; The second LIM domain of Isl2

smart00389
Location:191 → 247

HOX; Homeodomain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

55445961..55454951

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006510756.2 → XP_006510819.1 insulin gene enhancer protein ISL-2 isoform X1

Conserved Domains (3) summary

cd09366
Location:38 → 92

LIM1_Isl; The first LIM domain of Isl, a member of LHX protein family

cd09471
Location:100 → 154

LIM2_Isl2; The second LIM domain of Isl2

smart00389
Location:202 → 258

HOX; Homeodomain